LOCUS       Exported                4476 bp DNA     circular SYN 04-SEP-2024
DEFINITION  Exported.
ACCESSION   V004416
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Minton NP.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   J. Microbiol. Methods 78 (1), 79-85 (2009)
  PUBMED    19445976
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Pennington OJ, Heap JT, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The 
            University of Nottingham, University Park, Nottingham NG7 2RD, 
            United Kingdom
REFERENCE   3  (bases 1 to 4476)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4476)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4476)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2009"; volume: "78"; issue: "1"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2009) Centre for Biomolecular Sciences, The University of 
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /note="terminator derived from CD0164 gene of Clostridium 
                     difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      363..404
                     /note="terminator derived from fdx gene of Clostridium 
                     pasteurianum"
                     /regulatory_class="terminator"
     CDS             1131..1448
                     /codon_start=1
                     /gene="repH"
                     /product="putative plasmid replication protein"
                     /label=repH
                     /protein_id="ACR43897.1"
                     /translation="MKRRAYMKTCKNCKEFIKDTEICKIHSLMIHDKTVATYCSKYNES
                     RCLHKGKVQCINCSKMNRYGWCAIKMRCFTEEEQKKERTCIKYYARSFKKAHVKKSKKK
                     K"
     gene            1131..1448
                     /gene="repH"
                     /label=repH
     CDS             2120..2740
                     /gene="catP"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from Clostridium 
                     perfringens. Accession#: P26826"
     rep_origin      2920..3508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            3829..3938
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             3971..4339
                     /label=traJ
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
      121 ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg
      181 cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgata
      361 gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgccattat
      421 ttttttgaac aattgacaat tcatttctta ttttttatta agtgatagtc aaaaggcata
      481 acagtgctga atagaaagaa atttacagaa aagaaaatta tagaatttag tatgattaat
      541 tatactcatt tatgaatgtt taattgaata caaaaaaaaa tacttgttat gtattcaatt
      601 acgggttaaa atatagacaa gttgaaaaat ttaataaaaa aataagtcct cagctcttat
      661 atattaagct accaacttag tatataagcc aaaacttaaa tgtgctacca acacatcaag
      721 ccgttagaga actctatcta tagcaatatt tcaaatgtac cgacatacaa gagaaacatt
      781 aactatatat attcaattta tgagattatc ttaacagata taaatgtaaa ttgcaataag
      841 taagatttag aagtttatag cctttgtgta ttggaagcag tacgcaaagg cttttttatt
      901 tgataaaaat tagaagtata tttatttttt cataattaat ttatgaaaat gaaagggggt
      961 gagcaaagtg acagaggaaa gcagtatctt atcaaataac aaggtattag caatatcatt
     1021 attgacttta gcagtaaaca ttatgacttt tatagtgctt gtagctaagt agtacgaaag
     1081 ggggagcttt aaaaagctcc ttggaataca tagaattcat aaattaattt atgaaaagaa
     1141 gggcgtatat gaaaacttgt aaaaattgca aagagtttat taaagatact gaaatatgca
     1201 aaatacattc gttgatgatt catgataaaa cagtagcaac ctattgcagt aaatacaatg
     1261 agtcaagatg tttacataaa gggaaagtcc aatgtattaa ttgttcaaag atgaaccgat
     1321 atggatggtg tgccataaaa atgagatgtt ttacagagga agaacagaaa aaagaacgta
     1381 catgcattaa atattatgca aggagcttta aaaaagctca tgtaaagaag agtaaaaaga
     1441 aaaaataatt tatttattaa tttaatattg agagtgccga cacagtatgc actaaaaaat
     1501 atatctgtgg tgtagtgagc cgatacaaaa ggatagtcac tcgcattttc ataatacatc
     1561 ttatgttatg attatgtgtc ggtgggactt cacgacgaaa acccacaata aaaaaagagt
     1621 tcggggtagg gttaagcata gttgaggcaa ctaaacaatc aagctaggat atgcagtagc
     1681 agaccgtaag gtcgttgttt aggtgtgttg taatacatac gctattaaga tgtaaaaata
     1741 cggataccaa tgaagggaaa agtataattt ttggatgtag tttgtttgtt catctatggg
     1801 caaactacgt ccaaagccgt ttccaaatct gctaaaaagt atatcctttc taaaatcaaa
     1861 gtcaagtatg aaatcataaa taaagtttaa ttttgaagtt attatgatat tatgtttttc
     1921 tattaaaata aattaagtat atagaatagt ttaataatag tatatactta atgtgataag
     1981 tgtctgacag tgtcacagaa aggatgattg ttatggatta taagcggccg gccagtgggc
     2041 aagttgaaaa attcacaaaa atgtggtata atatctttgt tcattagagc gataaacttg
     2101 aatttgagag gggacttaga tggtatttga aaaaattgat aaaaatagtt ggaacagaaa
     2161 agagtatttt gaccactact ttgcaagtgt accttgtacc tacagcatga ccgttaaagt
     2221 ggatatcaca caaataaagg aaaagggaat gaaactatat cctgcaatgc tttattatat
     2281 tgcaatgatt gtaaaccgcc attcagagtt taggacggca atcaatcaag atggtgaatt
     2341 ggggatatat gatgagatga taccaagcta tacaatattt cacaatgata ctgaaacatt
     2401 ttccagcctt tggactgagt gtaagtctga ctttaaatca tttttagcag attatgaaag
     2461 tgatacgcaa cggtatggaa acaatcatag aatggaagga aagccaaatg ctccggaaaa
     2521 catttttaat gtatctatga taccgtggtc aaccttcgat ggctttaatc tgaatttgca
     2581 gaaaggatat gattatttga ttcctatttt tactatgggg aaatattata aagaagataa
     2641 caaaattata cttcctttgg caattcaagt tcatcacgca gtatgtgacg gatttcacat
     2701 ttgccgtttt gtaaacgaat tgcaggaatt gataaatagt taacttcagg tttgtctgta
     2761 actaaaaaca agtatttaag caaaaacatc gtagaaatac ggtgtttttt gttaccctaa
     2821 gtttaaactc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     3601 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     3661 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcagggccc cctgcttcgg
     3721 ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg
     3781 ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcaggatagg
     3841 tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt cgcacctggc
     3901 ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg taacagatga
     3961 gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac ctatcaaggt
     4021 gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg ccggcatgag
     4081 cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg tcgtggacta
     4141 tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg gcggcctgct
     4201 gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca cgatcctcgc
     4261 cctgctggcg aagatcgaag agaagcagga cgagcttggc aaggtcatga tgggcgtggt
     4321 ccgcccgagg gcagagccat gactttttta gccgctaaaa cggccggggg gtgcgcgtga
     4381 ttgccaagca cgtccccatg cgctccatca agaagagcga cttcgcggag ctggtgaagt
     4441 acatcaccga cgagcaaggc aagaccgatc gggccc
//
