LOCUS       Exported                5260 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Exported.
ACCESSION   V004418
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Minton NP.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   J. Microbiol. Methods 78 (1), 79-85 (2009)
  PUBMED    19445976
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Pennington OJ, Heap JT, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The 
            University of Nottingham, University Park, Nottingham NG7 2RD, 
            United Kingdom
REFERENCE   3  (bases 1 to 5260)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5260)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2009"; volume: "78"; issue: "1"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2009) Centre for Biomolecular Sciences, The University of 
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2801..2806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /note="terminator derived from CD0164 gene of Clostridium 
                     difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      363..404
                     /note="terminator derived from fdx gene of Clostridium 
                     pasteurianum"
                     /regulatory_class="terminator"
     CDS             925..1980
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label=repA
                     /protein_id="ACR43886.1"
                     /translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNIE
                     RTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRKEE
                     NKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQKEKY
                     ITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRERWLE
                     LWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKALKGKQV
                     LVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTEDEKEEL
                     NQDLIDEIEID"
     gene            925..1980
                     /gene="repA"
                     /label=repA
     CDS             complement(2071..2574)
                     /codon_start=1
                     /product="putative plasmid replication protein"
                     /label=putative plasmid replication protein
                     /note="orf2"
                     /protein_id="ACR43887.1"
                     /translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYVG
                     PEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFATVT
                     IKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFKKEL
                     KTKN"
     CDS             2904..3524
                     /gene="catP"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from Clostridium 
                     perfringens. Accession#: P26826"
     rep_origin      3704..4292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            4613..4722
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             4755..5123
                     /label=traJ
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
      121 ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg
      181 cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgata
      361 gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgttctgaa
      421 tccttagcta atggttcaac aggtaactat gacgaagata gcaccctgga taagtctgta
      481 atggattcta aggcatttaa tgaagacgtg tatataaaat gtgctaatga aaaagaaaat
      541 gcgttaaaag agcctaaaat gagttcaaat ggttttgaaa ttgattggta gtttaattta
      601 atatattttt tctattggct atctcgatac ctatagaatc ttctgttcac ttttgttttt
      661 gaaatataaa aaggggcttt ttagcccctt ttttttaaaa ctccggagga gtttcttcat
      721 tcttgatact atacgtaact attttcgatt tgacttcatt gtcaattaag ctagtaaaat
      781 caatggttaa aaaacaaaaa acttgcattt ttctacctag taatttataa ttttaagtgt
      841 cgagtttaaa agtataattt accaggaaag gagcaagttt tttaataagg aaaaattttt
      901 ccttttaaaa ttctatttcg ttatatgact aattataatc aaaaaaatga aaataaacaa
      961 gaggtaaaaa ctgctttaga gaaatgtact gataaaaaaa gaaaaaatcc tagatttacg
     1021 tcatacatag cacctttaac tactaagaaa aatattgaaa ggacttccac ttgtggagat
     1081 tatttgttta tgttgagtga tgcagactta gaacatttta aattacataa aggtaatttt
     1141 tgcggtaata gattttgtcc aatgtgtagt tggcgacttg cttgtaagga tagtttagaa
     1201 atatctattc ttatggagca tttaagaaaa gaagaaaata aagagtttat atttttaact
     1261 cttacaactc caaatgtaaa aagttatgat cttaattatt ctattaaaca atataataaa
     1321 tcttttaaaa aattaatgga gcgtaaggaa gttaaggata taactaaagg ttatataaga
     1381 aaattagaag taacttacca aaaggaaaaa tacataacaa aggatttatg gaaaataaaa
     1441 aaagattatt atcaaaaaaa aggacttgaa attggtgatt tagaacctaa ttttgatact
     1501 tataatcctc attttcatgt agttattgca gttaataaaa gttattttac agataaaaat
     1561 tattatataa atcgagaaag atggttggaa ttatggaagt ttgctactaa ggatgattct
     1621 ataactcaag ttgatgttag aaaagcaaaa attaatgatt ataaagaggt ttacgaactt
     1681 gcgaaatatt cagctaaaga cactgattat ttaatatcga ggccagtatt tgaaattttt
     1741 tataaagcat taaaaggcaa gcaggtatta gtttttagtg gattttttaa agatgcacac
     1801 aaattgtaca agcaaggaaa acttgatgtt tataaaaaga aagatgaaat taaatatgtc
     1861 tatatagttt attataattg gtgcaaaaaa caatatgaaa aaactagaat aagggaactt
     1921 acggaagatg aaaaagaaga attaaatcaa gatttaatag atgaaataga aatagattaa
     1981 agtgtaacta tactttatat atatatgatt aaaaaaataa aaaacaacag cctattaggt
     2041 tgttgttttt tattttcttt attaattttt ttaattttta gtttttagtt cttttttaaa
     2101 ataagtttca gcctcttttt caatattttt taaagaagga gtatttgcat gaattgcctt
     2161 ttttctaaca gacttaggaa atattttaac agtatcttct tgcgccggtg attttggaac
     2221 ttcataactt actaatttat aattattatt ttctttttta attgtaacag ttgcaaaaga
     2281 agctgaacct gttccttcaa ctagtttatc atcttcaata taatattctt gacctatata
     2341 gtataaatat atttttatta tatttttact tttttctgaa tctattattt tataatcata
     2401 aaaagtttta ccaccaaaag aaggttgtac tccttctggt ccaacatatt tttttactat
     2461 attatctaaa taatttttgg gaactggtgt tgtaatttga ttaatcgaac aaccagttat
     2521 acttaaagga attataacta taaaaatata taggattatc tttttaaatt tcattattgg
     2581 cctccttttt attaaattta tgttaccata aaaaggacat aacgggaata tgtagaatat
     2641 ttttaatgta gacaaaattt tacataaata taaagaaagg aagtgtttgt ttaaatttta
     2701 tagcaaacta tcaaaaatta gggggataaa aatttatgaa aaaaaggttt tcgatgttat
     2761 ttttatgttt aactttaata gtttgtggtt tatttacaaa atcgatttcg gccggccagt
     2821 gggcaagttg aaaaattcac aaaaatgtgg tataatatct ttgttcatta gagcgataaa
     2881 cttgaatttg agaggggact tagatggtat ttgaaaaaat tgataaaaat agttggaaca
     2941 gaaaagagta ttttgaccac tactttgcaa gtgtaccttg tacctacagc atgaccgtta
     3001 aagtggatat cacacaaata aaggaaaagg gaatgaaact atatcctgca atgctttatt
     3061 atattgcaat gattgtaaac cgccattcag agtttaggac ggcaatcaat caagatggtg
     3121 aattggggat atatgatgag atgataccaa gctatacaat atttcacaat gatactgaaa
     3181 cattttccag cctttggact gagtgtaagt ctgactttaa atcattttta gcagattatg
     3241 aaagtgatac gcaacggtat ggaaacaatc atagaatgga aggaaagcca aatgctccgg
     3301 aaaacatttt taatgtatct atgataccgt ggtcaacctt cgatggcttt aatctgaatt
     3361 tgcagaaagg atatgattat ttgattccta tttttactat ggggaaatat tataaagaag
     3421 ataacaaaat tatacttcct ttggcaattc aagttcatca cgcagtatgt gacggatttc
     3481 acatttgccg ttttgtaaac gaattgcagg aattgataaa tagttaactt caggtttgtc
     3541 tgtaactaaa aacaagtatt taagcaaaaa catcgtagaa atacggtgtt ttttgttacc
     3601 ctaagtttaa actccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcagg gccccctgct
     4501 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     4561 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     4621 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     4681 tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag
     4741 atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca
     4801 aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca
     4861 tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg
     4921 actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc
     4981 tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc
     5041 tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg
     5101 tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc
     5161 gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg
     5221 aagtacatca ccgacgagca aggcaagacc gatcgggccc
//
