LOCUS       V004426                11845 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004426
VERSION     V004426
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP.
  TITLE     The ClosTron: a universal gene knock-out system for the genus
            Clostridium
  JOURNAL   J. Microbiol. Methods 70 (3), 452-464 (2007)
   PUBMED   17658189
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Centre for Biomolecular Sciences, The
            University of Nottingham, University Park, Nottingham NG7 2RD,
            United Kingdom
REFERENCE   3  (bases 1 to 11845)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11845)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2007"; volume: "70"; issue: "3"; pages:
            "452-464"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-MAR-2007) Centre for Biomolecular Sciences, The University of
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..338
                     /note="group II intron IBS and EBS; replaced to alter the
                     intron target specificity"
     CDS             complement(743..1477)
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     intron          1510..1902
                     /gene="ErmBtdRAM2"
                     /note="group I intron from td gene of phage T4 with
                     intron-encoded protein deletion; inserted in reverse
                     orientation to confer RAM functionality"
     RBS             2237..2259
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2451..4247
                     /gene="ltrA"
                     /label="Group II intron-encoded protein LtrA"
                     /note="Group II intron-encoded protein LtrA from
                     Lactococcus lactis subsp. cremoris (strain MG1363).
                     Accession#: P0A3U1"
     terminator      4436..4522
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4614..4641
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     complement(4888..4904)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5784..6101
                     /codon_start=1
                     /product="putative plasmid replication protein"
                     /label="putative plasmid replication protein"
                     /note="ORFH"
                     /protein_id="ABU90834.1"
                     /translation="MKRRAYMKTCKNCKEFIKDTEICKIHSLMIHDKTVATYCSKYNES
                     RCLHKGKVQCINCSKMNRYGWCAIKMRCFTEEEQKKERTCIKYYARSFKKAHVKKSKKK
                     K"
     CDS             6893..7513
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      7998..8586
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8880..9959)
                     /label="lacI"
                     /note="lac repressor"
     oriT            10671..10780
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             10813..11181
                     /label="traJ"
                     /note="oriT-recognizing protein"
     regulatory      11585..11686
                     /label="IPTG-inducible promoter fac"
                     /note="IPTG-inducible promoter fac"
                     /regulatory_class="promoter"
     protein_bind    complement(11692..11716)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             11735..11743
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agcttataat tatccttacg tgacggttaa gtgcgcccag atagggtgtt aagtcaagta
       61 gtttaaggta ctactctgta agataacaca gaaaacagcc aacctaaccg aaaagcgaaa
      121 gctgatacgg gaacagagca cggttggaaa gcgatgagtt acctaaagac aatcgggtac
      181 gactgagtcg caatgttaat cagatataag gtataagttg tgtttactga acgcaagttt
      241 ctaatttcgg tttcacgtcg atagaggaaa gtgtctgaaa cctctagtac aaagaaaggt
      301 aagttacgtt aaccgactta tctgttatca ccacatttgt acaatctgta ggagaaccta
      361 tgggaacgaa acgaaagcga tgccgagaat ctgaatttac caagacttaa cactaactgg
      421 ggatacccta aacaagaatg cctaatagaa aggaggaaaa aggctatagc actagagctt
      481 gaaaatcttg caagggtacg gagtactcgt agtagtctga gaagggtaac gccctttaca
      541 tggcaaaggg gtacagttat tgtgtactaa aattaaaaat tgattaggga ggaaaacctc
      601 aaaatgaaac caacaatggc aattttagaa agaatcagta aaaattcaca agaaaatata
      661 gacgaagttt ttacaagact ttatcgttat cttttacgtc cagatattta ttacgtggcg
      721 acgcgtgcga ctcatagaat tatttcctcc cgttaaataa tagataacta ttaaaaatag
      781 acaatacttg ctcataagta acggtactta aattgtttac tttggcgtgt ttcattgctt
      841 gatgaaactg atttttagta aacagttgac gatattctcg attgacccat tttgaaacaa
      901 agtacgtata tagcttccaa tatttatctg gaacatctgt ggtatggcgg gtaagtttta
      961 ttaagacact gtttactttt ggtttaggat gaaagcattc cgctggcagc ttaagcaatt
     1021 gctgaatcga gacttgagtg tgcaagagca accctagtgt tcggtgaata tccaaggtac
     1081 gcttgtagaa tccttcttca acaatcagat agatgtcaga cgcatggctt tcaaaaacca
     1141 cttttttaat aatttgtgtg cttaaatggt aaggaatact cccaacaatt ttatacctct
     1201 gtttgttagg gaattgaaac tgtagaatat cttggtgaat taaagtgaca cgagtattca
     1261 gttttaattt ttctgacgat aagttgaata gatgactgtc taattcaata gacgttacct
     1321 gtttacttat tttagccagt ttcgtcgtta aatgcccttt acctgttcca atttcgtaaa
     1381 cggtatcggt ttcttttaaa ttcaattgtt ttattatttg gttgagtact ttttcactcg
     1441 ttaaaaagtt ttgagaatat tttatatttt tgttcatacc agcaccagaa gcaccagcat
     1501 ctcttgggtt aattgaggcc tgagtataag gtgacttata cttgtaatct atctaaacgg
     1561 ggaacctctc tagtagacaa tcccgtgcta aattgtagga ctgcccttta ataaatactt
     1621 ctatatttaa agaggtattt atgaaaagcg gaatttatca gattaaaaat actttctcta
     1681 gagaaaattt cgtctggatt agttacttat cgtgtaaaat ctgataaatg gaattggttc
     1741 tacataaatg cctaacgact atccctttgg ggagtagggt caagtgactc gaaacgatag
     1801 acaacttgct ttaacaagtt ggagatatag tctgctctgc atggtgacat gcagctggat
     1861 ataattccgg ggtaagatta acgaccttat ctgaacataa tgccatatga atccctccta
     1921 atttatacgt tttctctaac aacttaatta tacccactat tattattttt atcaatataa
     1981 cgcgttggga aatggcaatg atagcgaaac aacgtaaaac tcttgttgta tgctttcatt
     2041 gtcatcgtca cgtgattcat aaacacaagt gaatgtcgac agtgaatttt tacgaacgaa
     2101 caataacaga gccgtatact ccgagagggg tacgtacggt tcccgaagag ggtggtgcaa
     2161 accagtcaca gtaatgtgaa caaggcggta cctccctact tcaccatatc attttctgca
     2221 gccccctaga aataattttg tttaacttta agaaggagat atacatatat ggctagatcg
     2281 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc
     2341 aatttctatg cactcgtagt agtctgagaa gggtaacgcc ctttacatgg caaaggggta
     2401 cagttattgt gtactaaaat taaaaattga ttagggagga aaacctcaaa atgaaaccaa
     2461 caatggcaat tttagaaaga atcagtaaaa attcacaaga aaatatagac gaagttttta
     2521 caagacttta tcgttatctt ttacgtccag atatttatta cgtggcgtat caaaatttat
     2581 attccaataa aggagcttcc acaaaaggaa tattagatga tacagcggat ggctttagtg
     2641 aagaaaaaat aaaaaagatt attcaatctt taaaagacgg aacttactat cctcaacctg
     2701 tacgaagaat gtatattgca aaaaagaatt ctaaaaagat gagaccttta ggaattccaa
     2761 ctttcacaga taaattgatc caagaagctg tgagaataat tcttgaatct atctatgaac
     2821 cggtattcga agatgtgtct cacggtttta gacctcaacg aagctgtcac acagctttga
     2881 aaacaatcaa aagagagttt ggcggcgcaa gatggtttgt ggagggagat ataaaaggct
     2941 gcttcgataa tatagaccac gttacactca ttggactcat caatcttaaa atcaaagata
     3001 tgaaaatgag ccaattgatt tataaatttc taaaagcagg ttatctggaa aactggcagt
     3061 atcacaaaac ttacagcgga acacctcaag gtggaattct atctcctctt ttggccaaca
     3121 tctatcttca tgaattggat aagtttgttt tacaactcaa aatgaagttt gaccgagaaa
     3181 gtccagaaag aataacacct gaatatcggg agctccacaa tgagataaaa agaatttctc
     3241 accgtctcaa gaagttggag ggtgaagaaa aagctaaagt tcttttagaa tatcaagaaa
     3301 aacgtaaaag attacccaca ctcccctgta cctcacagac aaataaagta ttgaaatacg
     3361 tccggtatgc ggacgacttc attatctctg ttaaaggaag caaagaggac tgtcaatgga
     3421 taaaagaaca attaaaactt tttattcata acaagctaaa aatggaattg agtgaagaaa
     3481 aaacactcat cacacatagc agtcaacccg ctcgttttct gggatatgat atacgagtaa
     3541 ggagatctgg aacgataaaa cgatctggta aagtcaaaaa gagaacactc aatgggagtg
     3601 tagaactcct tattcctctt caagacaaaa ttcgtcaatt tatttttgac aagaaaatag
     3661 ctatccaaaa gaaagatagc tcatggtttc cagttcacag gaaatatctt attcgttcaa
     3721 cagacttaga aatcatcaca atttataatt ctgaactccg cgggatttgt aattactacg
     3781 gtctagcaag taattttaac cagctcaatt attttgctta tcttatggaa tacagctgtc
     3841 taaaaacgat agcctccaaa cataagggaa cactttcaaa aaccatttcc atgtttaaag
     3901 atggaagtgg ttcgtggggg atcccgtatg agataaagca aggtaagcag cgccgttatt
     3961 ttgcaaattt tagtgaatgt aaatcccctt atcaatttac ggatgagata agtcaagctc
     4021 ctgtattgta tggctatgcc cggaatactc ttgaaaacag gttaaaagct aaatgttgtg
     4081 aattatgtgg gacgtctgat gaaaatactt cctatgaaat tcaccatgtc aataaggtca
     4141 aaaatcttaa aggcaaagaa aaatgggaaa tggcaatgat agcgaaacaa cgtaaaactc
     4201 ttgttgtatg ctttcattgt catcgtcacg tgattcataa acacaagtga atgtcgagca
     4261 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     4321 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcgccaag
     4381 ctcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     4441 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     4501 cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     4561 cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     4621 catcctgacg gatggccttt ttgcgtttct acaaactctt cctgtcgtca tatctacaag
     4681 ccatcccccc acagatacgg taaactagcc tcgtttttgc atcaggaaag cagaacgcca
     4741 tgagcggcct catttcttat tctgagttac aacagtccgc accgctgtcc ggtagctcct
     4801 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     4861 aactcgtagg acaggtgcca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     4921 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4981 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5041 atggcgctag cgaagagatg cagcagccat tatttttttg aacaattgac aattcatttc
     5101 ttatttttta ttaagtgata gtcaaaaggc ataacagtgc tgaatagaaa gaaatttaca
     5161 gaaaagaaaa ttatagaatt tagtatgatt aattatactc atttatgaat gtttaattga
     5221 atacaaaaaa aaatacttgt tatgtattca attacgggtt aaaatataga caagttgaaa
     5281 aatttaataa aaaaataagt cctcagctct tatatattaa gctaccaact tagtatataa
     5341 gccaaaactt aaatgtgcta ccaacacatc aagccgttag agaactctat ctatagcaat
     5401 atttcaaatg taccgacata caagagaaac attaactata tatattcaat ttatgagatt
     5461 atcttaacag atataaatgt aaattgcaat aagtaagatt tagaagttta tagcctttgt
     5521 gtattggaag cagtacgcaa aggctttttt atttgataaa aattagaagt atatttattt
     5581 tttcataatt aatttatgaa aatgaaaggg ggtgagcaaa gtgacagagg aaagcagtat
     5641 cttatcaaat aacaaggtat tagcaatatc attattgact ttagcagtaa acattatgac
     5701 ttttatagtg cttgtagcta agtagtacga aagggggagc tttaaaaagc tccttggaat
     5761 acatagaatt cataaattaa tttatgaaaa gaagggcgta tatgaaaact tgtaaaaatt
     5821 gcaaagagtt tattaaagat actgaaatat gcaaaataca ttcgttgatg attcatgata
     5881 aaacagtagc aacctattgc agtaaataca atgagtcaag atgtttacat aaagggaaag
     5941 tccaatgtat taattgttca aagatgaacc gatatggatg gtgtgccata aaaatgagat
     6001 gttttacaga ggaagaacag aaaaaagaac gtacatgcat taaatattat gcaaggagct
     6061 ttaaaaaagc tcatgtaaag aagagtaaaa agaaaaaata atttatttat taatttaata
     6121 ttgagagtgc cgacacagta tgcactaaaa aatatatctg tggtgtagtg agccgataca
     6181 aaaggatagt cactcgcatt ttcataatac atcttatgtt atgattatgt gtcggtggga
     6241 cttcacgacg aaaacccaca ataaaaaaag agttcggggt agggttaagc atagttgagg
     6301 caactaaaca atcaagctag gatatgcagt agcagaccgt aaggtcgttg tttaggtgtg
     6361 ttgtaataca tacgctatta agatgtaaaa atacggatac caatgaaggg aaaagtataa
     6421 tttttggatg tagtttgttt gttcatctat gggcaaacta cgtccaaagc cgtttccaaa
     6481 tctgctaaaa agtatatcct ttctaaaatc aaagtcaagt atgaaatcat aaataaagtt
     6541 taattttgaa gttattatga tattatgttt ttctattaaa ataaattaag tatatagaat
     6601 agtttaataa tagtatatac ttaatgtgat aagtgtctga cagctgaccg gtctaaagag
     6661 gtccctagcg cctacgggga atttgtatcg ataaggggta caaattccca ctaagcgctc
     6721 ggcggggatc gatcccgggt acgtacccgg cagtttttct ttttcggcaa gtgttcaaga
     6781 agttattaag tcgggagtgc agtcgaagtg ggcaagttga aaaattcaca aaaatgtggt
     6841 ataatatctt tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt
     6901 tgaaaaaatt gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag
     6961 tgtaccttgt acctacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg
     7021 aatgaaacta tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga
     7081 gtttaggacg gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag
     7141 ctatacaata tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc
     7201 tgactttaaa tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca
     7261 tagaatggaa ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg
     7321 gtcaaccttc gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat
     7381 ttttactatg gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca
     7441 agttcatcac gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga
     7501 attgataaat agttaacttc aggtttgtct gtaactaaaa acaagtattt aagcaaaaac
     7561 atcgtagaaa tacggtgttt tttgttaccc taaaatctac aattttatac ataaccacag
     7621 gttagtacaa agaccttgtg tttctttttg aaaggcttaa aacaaggatt tttccttgat
     7681 ttaagccccg aaaagcaaca caaccaaggt tttagtatca atctgtggtt tttatatttt
     7741 cagagaccgg tcaggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7801 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7861 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7921 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7981 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     8401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8581 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8641 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8701 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8761 gcggaagcag taagacgggt aagcctgttg atgataccgc tgccttactg ggtgcattag
     8821 ccagtctgaa tgacctgtca cgggataatt cctaactcac attaattgcg ttgcgctcac
     8881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8941 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     9001 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     9061 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat
     9121 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     9181 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     9241 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     9301 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     9361 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     9421 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     9481 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     9541 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     9601 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     9661 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     9721 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     9781 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     9841 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     9901 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcata
     9961 tgttgcacct ctactttaat aatttttaac ttttatatat gattaattta attgtttgtt
    10021 aaatttatat caatcaatgc tatgaatatt tctttatacc ttattgtaac aaaaaaatat
    10081 tggaaatgtt gaattttcag aatattattt ttattatatt attaatttta tatattcatt
    10141 tttataagat ttcacaacac gaacgtaata taatatatct tcctcatctt ctgaaaagat
    10201 tatactaatt ctattcatgt tacttataat cttattttgg taaatcgaat ttttcaatta
    10261 tatgttcggc aacctttatc ccatcaacag ccgctgatat tataccacct gcaaatcctg
    10321 ccccttctcc agttggataa agtccgcata catttatact ttcaagtgaa gcatttctat
    10381 tcaatctaac tggtgctgat gttcttgtct caattcccgt taaaattgca tcttctcttg
    10441 catacccttt tatcttttta tcaaaattta taattccttc tttaagagcc tctacaacat
    10501 aatcaggtaa acattctttt aattccctga attatctgca gaattcgccc ttcctgcttc
    10561 ggggtcatta tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt
    10621 tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata
    10681 ggtgaagtag gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg
    10741 gcggtgctca acgggaatcc tgctctgcga ggctggccgg ctaccgccgg cgtaacagat
    10801 gagggcaagc ggatggctga tgaaaccaag ccaaccagga agggcagccc acctatcaag
    10861 gtgtactgcc ttccagacga acgaagagcg attgaggaaa aggcggcggc ggccggcatg
    10921 agcctgtcgg cctacctgct ggccgtcggc cagggctaca aaatcacggg cgtcgtggac
    10981 tatgagcacg tccgcgagct ggcccgcatc aatggcgacc tgggccgcct gggcggcctg
    11041 ctgaaactct ggctcaccga cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc
    11101 gccctgctgg cgaagatcga agagaagcag gacgagcttg gcaaggtcat gatgggcgtg
    11161 gtccgcccga gggcagagcc atgacttttt tagccgctaa aacggccggg gggtgcgcgt
    11221 gattgccaag cacgtcccca tgcgctccat caagaagagc gacttcgcgg agctggtgaa
    11281 gtacatcacc gacgagcaag gcaagaccga tccccatccc gaagtggtca gactggaaaa
    11341 tcagagggca ggaactgcga acagcaaaaa gtcagatagc accacatagc agacccgcca
    11401 taaaacgccc tgagagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg
    11461 aatccataaa aggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    11521 atgcagagct tactccccat ccccccagtg aattccccgg atcgagatag tatatgatgc
    11581 atattcttta aatatagata aagttataga agcaatagaa gatttaggat ttactgtaat
    11641 ataaattaca cttttaaaaa gtttaaaaac atgatacaat aagttatggt tggaattgtt
    11701 atccgctcac aattccaact tatgattaaa attttaagga ggtgtatttc atatgaccat
    11761 gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgtcac gcgtccatgg
    11821 agatctcgag gcctgcaggc atgca
//
