LOCUS       V012834                 6000 bp    DNA     circular SYN 04-JAN-2024
DEFINITION  Exported.
ACCESSION   V012834
VERSION     V012834
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(254..278)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             297..305
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             318..1598
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     primer_bind     complement(1723..1739)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2655..2972
                     /codon_start=1
                     /gene="repH"
                     /product="putative plasmid replication protein"
                     /label="repH"
                     /protein_id="ACR43897.1"
                     /translation="MKRRAYMKTCKNCKEFIKDTEICKIHSLMIHDKTVATYCSKYNES
                     RCLHKGKVQCINCSKMNRYGWCAIKMRCFTEEEQKKERTCIKYYARSFKKAHVKKSKKK
                     K"
     gene            2655..2972
                     /gene="repH"
                     /label="repH"
     CDS             3644..4264
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      4444..5032
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            5353..5462
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             5495..5863
                     /label="traJ"
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcccc
      121 ggatcgagat agtatatgat gcatattctt taaatataga taaagttata gaagcaatag
      181 aagatttagg atttactgta atataaatta cacttttaaa aagtttaaaa acatgataca
      241 ataagttatg gttggaattg ttatccgctc acaattccaa cttatgatta aaattttaag
      301 gaggtgtatt tcataatgtg tcgaataacg ctttacaaac aattattaac gcccggttac
      361 caggcgaaga ggggctgtgg cagattcatc tgcaggacgg aaaaatcagc gccattgatg
      421 cgcaatccgg cgtgatgccc ataactgaaa acagcctgga tgccgaacaa ggtttagtta
      481 taccgccgtt tgtggagcca catattcacc tggacaccac gcaaaccgcc ggacaaccga
      541 actggaatca gtccggcacg ctgtttgaag gcattgaacg ctgggccgag cgcaaagcgt
      601 tattaaccca tgacgatgtg aaacaacgcg catggcaaac gctgaaatgg cagattgcca
      661 acggcattca gcatgtgcgt acccatgtcg atgtttcgga tgcaacgcta actgcgctga
      721 aagcaatgct ggaagtgaag caggaagtcg cgccgtggat tgatctgcaa atcgtcgcct
      781 tccctcagga agggattttg tcgtatccca acggtgaagc gttgctggaa gaggcgttac
      841 gcttaggggc agatgtagtg ggggcgattc cgcattttga atttacccgt gaatacggcg
      901 tggagtcgct gcataaaacc ttcgccctgg cgcaaaaata cgaccgtctc atcgacgttc
      961 actgtgatga gatcgatgac gagcagtcgc gctttgtcga aaccgttgct gccctggcgc
     1021 accatgaagg catgggcgcg cgagtcaccg ccagccacac cacggcaatg cactcctata
     1081 acggggcgta tacctcacgc ctgttccgct tgctgaaaat gtccggtatt aactttgtcg
     1141 ccaacccgct ggtcaatatt catctgcaag gacgtttcga tacgtatcca aaacgtcgcg
     1201 gcatcacgcg cgttaaagag atgctggagt ccggcattaa cgtctgcttt ggtcacgatg
     1261 atgtcttcga tccgtggtat ccgctgggaa cggcgaatat gctgcaagtg ctgcatatgg
     1321 ggctgcatgt ttgccagttg atgggctacg ggcagattaa cgatggcctg aatttaatca
     1381 cccaccacag cgcaaggacg ttgaatttgc aggattacgg cattgccgcc ggaaacagcg
     1441 ccaacctgat tatcctgccg gctgaaaatg ggtttgatgc gctgcgccgt caggttccgg
     1501 tacgttattc ggtacgtggc ggcaaggtga ttgccagcac acaaccggca caaaccaccg
     1561 tatatctgga gcagccagaa gccatcgatt acaaacgttg aacgactggg ttacagcgag
     1621 cttagtttaa agggcgaatt cgagctcggt acccggggat cctctagagt cgacgtcacg
     1681 cgtccatgga gatctcgagg cctgcagaca tgcaagcttg gcactggccg tcgttttaca
     1741 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1801 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1861 cagcctgaat ggcgaatggc gctagcataa aaataagaag cctgcatttg caggcttctt
     1921 atttttatgg cgcgccgcca ttattttttt gaacaattga caattcattt cttatttttt
     1981 attaagtgat agtcaaaagg cataacagtg ctgaatagaa agaaatttac agaaaagaaa
     2041 attatagaat ttagtatgat taattatact catttatgaa tgtttaattg aatacaaaaa
     2101 aaaatacttg ttatgtattc aattacgggt taaaatatag acaagttgaa aaatttaata
     2161 aaaaaataag tcctcagctc ttatatatta agctaccaac ttagtatata agccaaaact
     2221 taaatgtgct accaacacat caagccgtta gagaactcta tctatagcaa tatttcaaat
     2281 gtaccgacat acaagagaaa cattaactat atatattcaa tttatgagat tatcttaaca
     2341 gatataaatg taaattgcaa taagtaagat ttagaagttt atagcctttg tgtattggaa
     2401 gcagtacgca aaggcttttt tatttgataa aaattagaag tatatttatt ttttcataat
     2461 taatttatga aaatgaaagg gggtgagcaa agtgacagag gaaagcagta tcttatcaaa
     2521 taacaaggta ttagcaatat cattattgac tttagcagta aacattatga cttttatagt
     2581 gcttgtagct aagtagtacg aaagggggag ctttaaaaag ctccttggaa tacatagaat
     2641 tcataaatta atttatgaaa agaagggcgt atatgaaaac ttgtaaaaat tgcaaagagt
     2701 ttattaaaga tactgaaata tgcaaaatac attcgttgat gattcatgat aaaacagtag
     2761 caacctattg cagtaaatac aatgagtcaa gatgtttaca taaagggaaa gtccaatgta
     2821 ttaattgttc aaagatgaac cgatatggat ggtgtgccat aaaaatgaga tgttttacag
     2881 aggaagaaca gaaaaaagaa cgtacatgca ttaaatatta tgcaaggagc tttaaaaaag
     2941 ctcatgtaaa gaagagtaaa aagaaaaaat aatttattta ttaatttaat attgagagtg
     3001 ccgacacagt atgcactaaa aaatatatct gtggtgtagt gagccgatac aaaaggatag
     3061 tcactcgcat tttcataata catcttatgt tatgattatg tgtcggtggg acttcacgac
     3121 gaaaacccac aataaaaaaa gagttcgggg tagggttaag catagttgag gcaactaaac
     3181 aatcaagcta ggatatgcag tagcagaccg taaggtcgtt gtttaggtgt gttgtaatac
     3241 atacgctatt aagatgtaaa aatacggata ccaatgaagg gaaaagtata atttttggat
     3301 gtagtttgtt tgttcatcta tgggcaaact acgtccaaag ccgtttccaa atctgctaaa
     3361 aagtatatcc tttctaaaat caaagtcaag tatgaaatca taaataaagt ttaattttga
     3421 agttattatg atattatgtt tttctattaa aataaattaa gtatatagaa tagtttaata
     3481 atagtatata cttaatgtga taagtgtctg acagtgtcac agaaaggatg attgttatgg
     3541 attataagcg gccggccagt gggcaagttg aaaaattcac aaaaatgtgg tataatatct
     3601 ttgttcatta gagcgataaa cttgaatttg agagggaact tagatggtat ttgaaaaaat
     3661 tgataaaaat agttggaaca gaaaagagta ttttgaccac tactttgcaa gtgtaccttg
     3721 tacctacagc atgaccgtta aagtggatat cacacaaata aaggaaaagg gaatgaaact
     3781 atatcctgca atgctttatt atattgcaat gattgtaaac cgccattcag agtttaggac
     3841 ggcaatcaat caagatggtg aattggggat atatgatgag atgataccaa gctatacaat
     3901 atttcacaat gatactgaaa cattttccag cctttggact gagtgtaagt ctgactttaa
     3961 atcattttta gcagattatg aaagtgatac gcaacggtat ggaaacaatc atagaatgga
     4021 aggaaagcca aatgctccgg aaaacatttt taatgtatct atgataccgt ggtcaacctt
     4081 cgatggcttt aatctgaatt tgcagaaagg atatgattat ttgattccta tttttactat
     4141 ggggaaatat tataaagaag ataacaaaat tatacttcct ttggcaattc aagttcatca
     4201 cgcagtatgt gacggatttc acatttgccg ttttgtaaac gaattgcagg aattgataaa
     4261 tagttaactt caggtttgtc tgtaactaaa aacaagtatt taagcaaaaa catcgtagaa
     4321 atacggtgtt ttttgttacc ctaagtttaa actccttttt gataatctca tgaccaaaat
     4381 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5101 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5161 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     5221 aatacgcagg gccccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     5281 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     5341 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     5401 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     5461 ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag
     5521 gaagggcagc ccacctatca aggtgtactg ccttccagac gaacgaagag cgattgagga
     5581 aaaggcggcg gcggccggca tgagcctgtc ggcctacctg ctggccgtcg gccagggcta
     5641 caaaatcacg ggcgtcgtgg actatgagca cgtccgcgag ctggcccgca tcaatggcga
     5701 cctgggccgc ctgggcggcc tgctgaaact ctggctcacc gacgacccgc gcacggcgcg
     5761 gttcggtgat gccacgatcc tcgccctgct ggcgaagatc gaagagaagc aggacgagct
     5821 tggcaaggtc atgatgggcg tggtccgccc gagggcagag ccatgacttt tttagccgct
     5881 aaaacggccg gggggtgcgc gtgattgcca agcacgtccc catgcgctcc atcaagaaga
     5941 gcgacttcgc ggagctggtg aagtacatca ccgacgagca aggcaagacc gatcgggccc
//
