LOCUS       V004427                 6778 bp    DNA     circular SYN 04-JAN-2024
DEFINITION  Exported.
ACCESSION   V004427
VERSION     V004427
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(254..278)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             297..305
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             318..1598
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     primer_bind     complement(1723..1739)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2449..3504
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label="repA"
                     /protein_id="ACR43886.1"
                     /translation="MTNYNQKNENKQEVKTALEKCTDKKRKNPRFTSYIAPLTTKKNIE
                     RTSTCGDYLFMLSDADLEHFKLHKGNFCGNRFCPMCSWRLACKDSLEISILMEHLRKEE
                     NKEFIFLTLTTPNVKSYDLNYSIKQYNKSFKKLMERKEVKDITKGYIRKLEVTYQKEKY
                     ITKDLWKIKKDYYQKKGLEIGDLEPNFDTYNPHFHVVIAVNKSYFTDKNYYINRERWLE
                     LWKFATKDDSITQVDVRKAKINDYKEVYELAKYSAKDTDYLISRPVFEIFYKALKGKQV
                     LVFSGFFKDAHKLYKQGKLDVYKKKDEIKYVYIVYYNWCKKQYEKTRIRELTEDEKEEL
                     NQDLIDEIEID"
     gene            2449..3504
                     /gene="repA"
                     /label="repA"
     CDS             complement(3595..4098)
                     /codon_start=1
                     /product="putative plasmid replication protein"
                     /label="putative plasmid replication protein"
                     /note="orf2"
                     /protein_id="ACR43887.1"
                     /translation="MKFKKIILYIFIVIIPLSITGCSINQITTPVPKNYLDNIVKKYVG
                     PEGVQPSFGGKTFYDYKIIDSEKSKNIIKIYLYYIGQEYYIEDDKLVEGTGSASFATVT
                     IKKENNNYKLVSYEVPKSPAQEDTVKIFPKSVRKKAIHANTPSLKNIEKEAETYFKKEL
                     KTKN"
     CDS             4422..5042
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      5222..5810
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            6131..6240
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             6273..6641
                     /label="traJ"
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcccc
      121 ggatcgagat agtatatgat gcatattctt taaatataga taaagttata gaagcaatag
      181 aagatttagg atttactgta atataaatta cacttttaaa aagtttaaaa acatgataca
      241 ataagttatg gttggaattg ttatccgctc acaattccaa cttatgatta aaattttaag
      301 gaggtgtatt tcataatgtg tcgaataacg ctttacaaac aattattaac gcccggttac
      361 caggcgaaga ggggctgtgg cagattcatc tgcaggacgg aaaaatcagc gccattgatg
      421 cgcaatccgg cgtgatgccc ataactgaaa acagcctgga tgccgaacaa ggtttagtta
      481 taccgccgtt tgtggagcca catattcacc tggacaccac gcaaaccgcc ggacaaccga
      541 actggaatca gtccggcacg ctgtttgaag gcattgaacg ctgggccgag cgcaaagcgt
      601 tattaaccca tgacgatgtg aaacaacgcg catggcaaac gctgaaatgg cagattgcca
      661 acggcattca gcatgtgcgt acccatgtcg atgtttcgga tgcaacgcta actgcgctga
      721 aagcaatgct ggaagtgaag caggaagtcg cgccgtggat tgatctgcaa atcgtcgcct
      781 tccctcagga agggattttg tcgtatccca acggtgaagc gttgctggaa gaggcgttac
      841 gcttaggggc agatgtagtg ggggcgattc cgcattttga atttacccgt gaatacggcg
      901 tggagtcgct gcataaaacc ttcgccctgg cgcaaaaata cgaccgtctc atcgacgttc
      961 actgtgatga gatcgatgac gagcagtcgc gctttgtcga aaccgttgct gccctggcgc
     1021 accatgaagg catgggcgcg cgagtcaccg ccagccacac cacggcaatg cactcctata
     1081 acggggcgta tacctcacgc ctgttccgct tgctgaaaat gtccggtatt aactttgtcg
     1141 ccaacccgct ggtcaatatt catctgcaag gacgtttcga tacgtatcca aaacgtcgcg
     1201 gcatcacgcg cgttaaagag atgctggagt ccggcattaa cgtctgcttt ggtcacgatg
     1261 atgtcttcga tccgtggtat ccgctgggaa cggcgaatat gctgcaagtg ctgcatatgg
     1321 ggctgcatgt ttgccagttg atgggctacg ggcagattaa cgatggcctg aatttaatca
     1381 cccaccacag cgcaaggacg ttgaatttgc aggattacgg cattgccgcc ggaaacagcg
     1441 ccaacctgat tatcctgccg gctgaaaatg ggtttgatgc gctgcgccgt caggttccgg
     1501 tacgttattc ggtacgtggc ggcaaggtga ttgccagcac acaaccggca caaaccaccg
     1561 tatatctgga gcagccagaa gccatcgatt acaaacgttg aacgactggg ttacagcgag
     1621 cttagtttaa agggcgaatt cgagctcggt acccggggat cctctagagt cgacgtcacg
     1681 cgtccatgga gatctcgagg cctgcagaca tgcaagcttg gcactggccg tcgttttaca
     1741 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1801 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1861 cagcctgaat ggcgaatggc gctagcataa aaataagaag cctgcatttg caggcttctt
     1921 atttttatgg cgcgccgttc tgaatcctta gctaatggtt caacaggtaa ctatgacgaa
     1981 gatagcaccc tggataagtc tgtaatggat tctaaggcat ttaatgaaga cgtgtatata
     2041 aaatgtgcta atgaaaaaga aaatgcgtta aaagagccta aaatgagttc aaatggtttt
     2101 gaaattgatt ggtagtttaa tttaatatat tttttctatt ggctatctcg atacctatag
     2161 aatcttctgt tcacttttgt ttttgaaata taaaaagggg ctttttagcc cctttttttt
     2221 aaaactccgg aggagtttct tcattcttga tactatacgt aactattttc gatttgactt
     2281 cattgtcaat taagctagta aaatcaatgg ttaaaaaaca aaaaacttgc atttttctac
     2341 ctagtaattt ataattttaa gtgtcgagtt taaaagtata atttaccagg aaaggagcaa
     2401 gttttttaat aaggaaaaat ttttcctttt aaaattctat ttcgttatat gactaattat
     2461 aatcaaaaaa atgaaaataa acaagaggta aaaactgctt tagagaaatg tactgataaa
     2521 aaaagaaaaa atcctagatt tacgtcatac atagcacctt taactactaa gaaaaatatt
     2581 gaaaggactt ccacttgtgg agattatttg tttatgttga gtgatgcaga cttagaacat
     2641 tttaaattac ataaaggtaa tttttgcggt aatagatttt gtccaatgtg tagttggcga
     2701 cttgcttgta aggatagttt agaaatatct attcttatgg agcatttaag aaaagaagaa
     2761 aataaagagt ttatattttt aactcttaca actccaaatg taaaaagtta tgatcttaat
     2821 tattctatta aacaatataa taaatctttt aaaaaattaa tggagcgtaa ggaagttaag
     2881 gatataacta aaggttatat aagaaaatta gaagtaactt accaaaagga aaaatacata
     2941 acaaaggatt tatggaaaat aaaaaaagat tattatcaaa aaaaaggact tgaaattggt
     3001 gatttagaac ctaattttga tacttataat cctcattttc atgtagttat tgcagttaat
     3061 aaaagttatt ttacagataa aaattattat ataaatcgag aaagatggtt ggaattatgg
     3121 aagtttgcta ctaaggatga ttctataact caagttgatg ttagaaaagc aaaaattaat
     3181 gattataaag aggtttacga acttgcgaaa tattcagcta aagacactga ttatttaata
     3241 tcgaggccag tatttgaaat tttttataaa gcattaaaag gcaagcaggt attagttttt
     3301 agtggatttt ttaaagatgc acacaaattg tacaagcaag gaaaacttga tgtttataaa
     3361 aagaaagatg aaattaaata tgtctatata gtttattata attggtgcaa aaaacaatat
     3421 gaaaaaacta gaataaggga acttacggaa gatgaaaaag aagaattaaa tcaagattta
     3481 atagatgaaa tagaaataga ttaaagtgta actatacttt atatatatat gattaaaaaa
     3541 ataaaaaaca acagcctatt aggttgttgt tttttatttt ctttattaat ttttttaatt
     3601 tttagttttt agttcttttt taaaataagt ttcagcctct ttttcaatat tttttaaaga
     3661 aggagtattt gcatgaattg ccttttttct aacagactta ggaaatattt taacagtatc
     3721 ttcttgcgcc ggtgattttg gaacttcata acttactaat ttataattat tattttcttt
     3781 tttaattgta acagttgcaa aagaagctga acctgttcct tcaactagtt tatcatcttc
     3841 aatataatat tcttgaccta tatagtataa atatattttt attatatttt tacttttttc
     3901 tgaatctatt attttataat cataaaaagt tttaccacca aaagaaggtt gtactccttc
     3961 tggtccaaca tattttttta ctatattatc taaataattt ttgggaactg gtgttgtaat
     4021 ttgattaatc gaacaaccag ttatacttaa aggaattata actataaaaa tatataggat
     4081 tatcttttta aatttcatta ttggcctcct ttttattaaa tttatgttac cataaaaagg
     4141 acataacggg aatatgtaga atatttttaa tgtagacaaa attttacata aatataaaga
     4201 aaggaagtgt ttgtttaaat tttatagcaa actatcaaaa attaggggga taaaaattta
     4261 tgaaaaaaag gttttcgatg ttatttttat gtttaacttt aatagtttgt ggtttattta
     4321 caaattcggc cggccagtgg gcaagttgaa aaattcacaa aaatgtggta taatatcttt
     4381 gttcattaga gcgataaact tgaatttgag agggaactta gatggtattt gaaaaaattg
     4441 ataaaaatag ttggaacaga aaagagtatt ttgaccacta ctttgcaagt gtaccttgta
     4501 cctacagcat gaccgttaaa gtggatatca cacaaataaa ggaaaaggga atgaaactat
     4561 atcctgcaat gctttattat attgcaatga ttgtaaaccg ccattcagag tttaggacgg
     4621 caatcaatca agatggtgaa ttggggatat atgatgagat gataccaagc tatacaatat
     4681 ttcacaatga tactgaaaca ttttccagcc tttggactga gtgtaagtct gactttaaat
     4741 catttttagc agattatgaa agtgatacgc aacggtatgg aaacaatcat agaatggaag
     4801 gaaagccaaa tgctccggaa aacattttta atgtatctat gataccgtgg tcaaccttcg
     4861 atggctttaa tctgaatttg cagaaaggat atgattattt gattcctatt tttactatgg
     4921 ggaaatatta taaagaagat aacaaaatta tacttccttt ggcaattcaa gttcatcacg
     4981 cagtatgtga cggatttcac atttgccgtt ttgtaaacga attgcaggaa ttgataaata
     5041 gttaacttca ggtttgtctg taactaaaaa caagtattta agcaaaaaca tcgtagaaat
     5101 acggtgtttt ttgttaccct aagtttaaac tcctttttga taatctcatg accaaaatcc
     5161 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5221 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5281 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5341 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5401 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5461 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5521 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5581 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5641 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5701 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5761 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5821 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5881 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5941 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6001 tacgcagggc cccctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     6061 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     6121 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     6181 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgg
     6241 ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag ccaaccagga
     6301 agggcagccc acctatcaag gtgtactgcc ttccagacga acgaagagcg attgaggaaa
     6361 aggcggcggc ggccggcatg agcctgtcgg cctacctgct ggccgtcggc cagggctaca
     6421 aaatcacggg cgtcgtggac tatgagcacg tccgcgagct ggcccgcatc aatggcgacc
     6481 tgggccgcct gggcggcctg ctgaaactct ggctcaccga cgacccgcgc acggcgcggt
     6541 tcggtgatgc cacgatcctc gccctgctgg cgaagatcga agagaagcag gacgagcttg
     6601 gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc atgacttttt tagccgctaa
     6661 aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat caagaagagc
     6721 gacttcgcgg agctggtgaa gtacatcacc gacgagcaag gcaagaccga tcgggccc
//
