LOCUS       V004431                 3705 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004431
VERSION     V004431
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton
            NP.
  TITLE     Integration of DNA into bacterial chromosomes from plasmids without
            a counter-selection marker
  JOURNAL   Nucleic Acids Res. 40 (8), E59 (2012)
   PUBMED   22259038
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) School of Molecular Medical Sciences, The
            University of Nottingham, Centre for Biomolecular Sciences,
            University Park, Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 3705)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JAN-2011) School of Molecular Medical Sciences, The University
            of Nottingham, Centre for Biomolecular Sciences, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            9..822
                     /gene="pyrF fragment"
                     /label="pyrF fragment"
     misc_feature    9..306
                     /gene="pyrF fragment"
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     primer_bind     complement(941..957)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1105..1146
                     /label="derived from fdx gene of Clostridium pasteurianum"
                     /note="derived from fdx gene of Clostridium pasteurianum"
                     /regulatory_class="terminator"
     CDS             1525..1965
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="AFA34929.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            1525..1965
                     /gene="repL"
                     /label="repL"
     CDS             2115..2735
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      2915..3503
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcagggt gtttaggact tgatactgat attacttatg taccagaaga gttttgtaag
       61 aaatttaata gtatagaaga tgctatattt aattttaata agaaaataat tgatgcgact
      121 ttagatgttg tttcatgtta taaggtgcaa attgcatatt atgaagctta tggtttaaaa
      181 ggacttttgg cctataaaag gacacttgaa tatttgagag aaaaaaaggc tattgcaatt
      241 gctgatataa aaagaggaga tatagctaaa acagctgaaa tgtatgctaa agctcacttt
      301 gaaggtgatt ttgaagcgga ttttgttacg ttaaatcctt acatggggtt agatggtata
      361 gagccttata tgccttatat tgaaaaaatg gaaaaaggat tatttatttt gcttagaaca
      421 tcgaataaag gagcctatga tatacaatat ataaagactc agggcggaaa aaacgtatat
      481 gatgaggttg gagaaaaaat atatgattta ggtcaaaagg ctacgggaag gagcaagtat
      541 tcttcaatag gagcagtagt tggatgtact cacgttgaag aaggcgttga aattagaaat
      601 aaatttaaaa atatgttttt tctaattcca ggctatggag cacaaggtgg aactgcaaag
      661 gaagtaagtt tgtatttaag agaaggtaat ggtggagtgg taaattcctc aaggggaata
      721 cttcttgctt ataaaaaaga agaaaacggt gaaaaaatat ttgatgagtg tgcaaggctt
      781 gcagcgatta atatgagaga cgagatcaga aaaactttat gagcggccgc tgtatccata
      841 tgaccatgat tacgaattcg agctcggtac ccggggatcc tctagagtcg acgtcacgcg
      901 tccatggaga tctcgaggcc tgcagacatg caagcttggc actggccgtc gttttacaac
      961 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     1021 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1081 gcctgaatgg cgaatggcgc tagcataaaa ataagaagcc tgcatttgca ggcttcttat
     1141 ttttatggcg cgccgcattc acttcttttc tatataaata tgagcgaagc gaataagcgt
     1201 cggaaaagca gcaaaaagtt tcctttttgc tgttggagca tgggggttca gggggtgcag
     1261 tatctgacgt caatgccgag cgaaagcgag ccgaagggta gcatttacgt tagataaccc
     1321 cctgatatgc tccgacgctt tatatagaaa agaagattca actaggtaaa atcttaatat
     1381 aggttgagat gataaggttt ataaggaatt tgtttgttct aatttttcac tcattttgtt
     1441 ctaatttctt ttaacaaatg ttcttttttt tttagaacag ttatgatata gttagaatag
     1501 tttaaaataa ggagtgagaa aaagatgaaa gaaagatatg gaacagtcta taaaggctct
     1561 cagaggctca tagacgaaga aagtggagaa gtcatagagg tagacaagtt ataccgtaaa
     1621 caaacgtctg gtaacttcgt aaaggcatat atagtgcaat taataagtat gttagatatg
     1681 attggcggaa aaaaacttaa aatcgttaac tatatcctag ataatgtcca cttaagtaac
     1741 aatacaatga tagctacaac aagagaaata gcaaaagcta caggaacaag tctacaaaca
     1801 gtaataacaa cacttaaaat cttagaagaa ggaaatatta taaaaagaaa aactggagta
     1861 ttaatgttaa accctgaact actaatgaga ggcgacgacc aaaaacaaaa atacctctta
     1921 ctcgaatttg ggaactttga gcaagaggca aatgaaatag attgacctcc caataacacc
     1981 acgtagttat tgggaggtca atctatgaaa tgcgattaag ggccggccag tgggcaagtt
     2041 gaaaaattca caaaaatgtg gtataatatc tttgttcatt agagcgataa acttgaattt
     2101 gagagggaac ttagatggta tttgaaaaaa ttgataaaaa tagttggaac agaaaagagt
     2161 attttgacca ctactttgca agtgtacctt gtacctacag catgaccgtt aaagtggata
     2221 tcacacaaat aaaggaaaag ggaatgaaac tatatcctgc aatgctttat tatattgcaa
     2281 tgattgtaaa ccgccattca gagtttagga cggcaatcaa tcaagatggt gaattgggga
     2341 tatatgatga gatgatacca agctatacaa tatttcacaa tgatactgaa acattttcca
     2401 gcctttggac tgagtgtaag tctgacttta aatcattttt agcagattat gaaagtgata
     2461 cgcaacggta tggaaacaat catagaatgg aaggaaagcc aaatgctccg gaaaacattt
     2521 ttaatgtatc tatgataccg tggtcaacct tcgatggctt taatctgaat ttgcagaaag
     2581 gatatgatta tttgattcct atttttacta tggggaaata ttataaagaa gataacaaaa
     2641 ttatacttcc tttggcaatt caagttcatc acgcagtatg tgacggattt cacatttgcc
     2701 gttttgtaaa cgaattgcag gaattgataa atagttaact tcaggtttgt ctgtaactaa
     2761 aaacaagtat ttaagcaaaa acatcgtaga aatacggtgt tttttgttac cctaagttta
     2821 aactcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2881 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2941 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3001 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3061 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3121 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3181 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3241 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3301 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3361 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3421 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3481 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3541 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3601 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3661 agtcagtgag cgaggaagcg gaagagcgcc caatacgcag ggccc
//
