LOCUS       V004441                 5192 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004441
VERSION     V004441
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton
            NP.
  TITLE     Integration of DNA into bacterial chromosomes from plasmids without
            a counter-selection marker
  JOURNAL   Nucleic Acids Res. 40 (8), E59 (2012)
   PUBMED   22259038
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) School of Molecular Medical Sciences, The
            University of Nottingham, Centre for Biomolecular Sciences,
            University Park, Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 5192)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5192)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JAN-2011) School of Molecular Medical Sciences, The University
            of Nottingham, Centre for Biomolecular Sciences, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /label="derived from CD0164 gene of Clostridium difficile"
                     /note="derived from CD0164 gene of Clostridium difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    90..389
                     /gene="thl fragment"
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     gene            90..389
                     /gene="thl fragment"
                     /label="thl fragment"
     CDS             413..1147
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     complement(1270..1286)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1434..2633
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     CDS             1869..2234
                     /codon_start=1
                     /gene="CAC2872"
                     /product="putative FoF1-type ATP synthase operon membrane
                     protein"
                     /label="CAC2872"
                     /protein_id="AFA34953.1"
                     /translation="MNLNIKRMLKVVTLYDAIIAAIVSVILLFAANYKISLIVIIGIFS
                     AIFNFYLSNLTADFVFVKKMGNTSLIFLSSIFRVILVFFIGIILYKIYKYYLIAYLGGY
                     SAHFIALIIYGSLVNKR"
     gene            1869..2234
                     /gene="CAC2872"
                     /label="CAC2872"
     CDS             3012..3452
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="AFA34954.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            3012..3452
                     /gene="repL"
                     /label="repL"
     CDS             3602..4222
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      4402..4990
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccatct atgcaacaaa agcagctatt gaaaaagcag
      121 gttggacagt tgatgaatta gatttaatag aatcaaatga agcttttgca gctcaaagtt
      181 tagcagtagc aaaagattta aaatttgata tgaataaagt aaatgtaaat ggaggagcta
      241 ttgcccttgg tcatccaatt ggagcatcag gtgcaagaat actcgttact cttgtacacg
      301 caatgcaaaa aagagatgca aaaaaaggct tagcaacttt atgtataggt ggcggacaag
      361 gaacagcaat attgctagaa aagtgctaga tcgattaaga aggagtgatt acatgaacaa
      421 aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa
      481 acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt
      541 aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct
      601 attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa ttcaccaaga
      661 tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga gtattcctta
      721 ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta
      781 tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt
      841 gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca
      901 tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc
      961 agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg
     1021 tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt
     1081 aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg
     1141 gaggaaataa agcggccgct gtatccatat gaccatgatt acgaattcga gctcggtacc
     1201 cggggatcct ctagagtcga cgtcacgcgt ccatggagat ctcgaggcct gcagacatgc
     1261 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1321 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1381 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct agcaaagtat
     1441 tgttaaaaat aactctgtag aattataaat tagttctaca gagttatttt ttaaaaaaat
     1501 tctaaactta tgtataaaaa atacgataag aatgtagaat taaaactaaa gacagttcaa
     1561 tttcttttag aataatttag ttagtgtggt aaaaaaatgt cataatgata tttatgttga
     1621 aatttgtata aaattcagaa aatgaatata ttttatcaat tttcagtcat ttgaaagatt
     1681 atgaggctaa tgcagtacta ggcgtaaatt gaatttataa ttactatagc gataagaaat
     1741 ggcctaaaaa cgtttgcagt aatgaaagaa ccgtaaatat tataaaaaaa atcttaaaac
     1801 agagttttat ttataaaaat ttaagatata taatttaaat aacgtgttaa aatagtggag
     1861 gaagtaattt gaatctgaat attaaaagaa tgttaaaggt tgtaactctt tatgatgcaa
     1921 ttattgctgc aatagtttca gtaatacttt tgtttgctgc taattataag atttcgttaa
     1981 tagtgattat agggattttt tcagcaatat ttaattttta tttaagtaat ttaacagctg
     2041 atttcgtttt tgtaaaaaaa atgggaaata cgtcacttat atttcttagt tcaattttta
     2101 gagtaatact tgtttttttt ataggtatta ttctttataa aatatataaa tattatttaa
     2161 tagcctactt aggaggatat agtgctcatt ttatagccct tataatttat gggtcactag
     2221 taaataaacg atgaaaggaa gtgattgaat ggagctaggt gcaaagacag tattttcgat
     2281 gaagcttgga agttacaact ttgctataac agaaactgta gtattacagt ggattatcat
     2341 ggcagttata atattacttg caatatttct tactaaaaat cttaagaaag taccaaatag
     2401 gaaacaaagc gtaatagaaa tgattgttaa cttaataaat ggattggtaa aagaaaatat
     2461 gggagagaaa ttcatgaatt tcgttccaat tatcggtact atggcagtgt ttatactttt
     2521 cttaaattta acagggctag taggtatcga accagcaaca aaggatatta gtgttacagc
     2581 aggctttgct ttagtaagtg catttttaat aaatgcaact gcaataaaaa gaaggcgcgc
     2641 cgcattcact tcttttctat ataaatatga gcgaagcgaa taagcgtcgg aaaagcagca
     2701 aaaagtttcc tttttgctgt tggagcatgg gggttcaggg ggtgcagtat ctgacgtcaa
     2761 tgccgagcga aagcgagccg aagggtagca tttacgttag ataaccccct gatatgctcc
     2821 gacgctttat atagaaaaga agattcaact aggtaaaatc ttaatatagg ttgagatgat
     2881 aaggtttata aggaatttgt ttgttctaat ttttcactca ttttgttcta atttctttta
     2941 acaaatgttc tttttttttt agaacagtta tgatatagtt agaatagttt aaaataagga
     3001 gtgagaaaaa gatgaaagaa agatatggaa cagtctataa aggctctcag aggctcatag
     3061 acgaagaaag tggagaagtc atagaggtag acaagttata ccgtaaacaa acgtctggta
     3121 acttcgtaaa ggcatatata gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa
     3181 aacttaaaat cgttaactat atcctagata atgtccactt aagtaacaat acaatgatag
     3241 ctacaacaag agaaatagca aaagctacag gaacaagtct acaaacagta ataacaacac
     3301 ttaaaatctt agaagaagga aatattataa aaagaaaaac tggagtatta atgttaaacc
     3361 ctgaactact aatgagaggc gacgaccaaa aacaaaaata cctcttactc gaatttggga
     3421 actttgagca agaggcaaat gaaatagatt gacctcccaa taacaccacg tagttattgg
     3481 gaggtcaatc tatgaaatgc gattaagggc cggccagtgg gcaagttgaa aaattcacaa
     3541 aaatgtggta taatatcttt gttcattaga gcgataaact tgaatttgag agggaactta
     3601 gatggtattt gaaaaaattg ataaaaatag ttggaacaga aaagagtatt ttgaccacta
     3661 ctttgcaagt gtaccttgta cctacagcat gaccgttaaa gtggatatca cacaaataaa
     3721 ggaaaaggga atgaaactat atcctgcaat gctttattat attgcaatga ttgtaaaccg
     3781 ccattcagag tttaggacgg caatcaatca agatggtgaa ttggggatat atgatgagat
     3841 gataccaagc tatacaatat ttcacaatga tactgaaaca ttttccagcc tttggactga
     3901 gtgtaagtct gactttaaat catttttagc agattatgaa agtgatacgc aacggtatgg
     3961 aaacaatcat agaatggaag gaaagccaaa tgctccggaa aacattttta atgtatctat
     4021 gataccgtgg tcaaccttcg atggctttaa tctgaatttg cagaaaggat atgattattt
     4081 gattcctatt tttactatgg ggaaatatta taaagaagat aacaaaatta tacttccttt
     4141 ggcaattcaa gttcatcacg cagtatgtga cggatttcac atttgccgtt ttgtaaacga
     4201 attgcaggaa ttgataaata gttaacttca ggtttgtctg taactaaaaa caagtattta
     4261 agcaaaaaca tcgtagaaat acggtgtttt ttgttaccct aagtttaaac tcctttttga
     4321 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4381 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4441 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4501 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4561 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4621 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4681 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4741 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4801 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4861 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4921 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4981 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5041 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5101 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5161 ggaagcggaa gagcgcccaa tacgcagggc cc
//
