LOCUS       V004442                 4034 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004442
VERSION     V004442
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton
            NP.
  TITLE     Integration of DNA into bacterial chromosomes from plasmids without
            a counter-selection marker
  JOURNAL   Nucleic Acids Res. 40 (8), E59 (2012)
   PUBMED   22259038
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) School of Molecular Medical Sciences, The
            University of Nottingham, Centre for Biomolecular Sciences,
            University Park, Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 4034)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JAN-2011) School of Molecular Medical Sciences, The University
            of Nottingham, Centre for Biomolecular Sciences, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /label="derived from CD0164 gene of Clostridium difficile"
                     /note="derived from CD0164 gene of Clostridium difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    90..389
                     /gene="thl fragment"
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     gene            90..389
                     /gene="thl fragment"
                     /label="thl fragment"
     CDS             413..1147
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     complement(1270..1286)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      1434..1475
                     /label="derived from fdx gene of Clostridium pasteurianum"
                     /note="derived from fdx gene of Clostridium pasteurianum"
                     /regulatory_class="terminator"
     CDS             1854..2294
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="AFA34949.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            1854..2294
                     /gene="repL"
                     /label="repL"
     CDS             2444..3064
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      3244..3832
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccatct atgcaacaaa agcagctatt gaaaaagcag
      121 gttggacagt tgatgaatta gatttaatag aatcaaatga agcttttgca gctcaaagtt
      181 tagcagtagc aaaagattta aaatttgata tgaataaagt aaatgtaaat ggaggagcta
      241 ttgcccttgg tcatccaatt ggagcatcag gtgcaagaat actcgttact cttgtacacg
      301 caatgcaaaa aagagatgca aaaaaaggct tagcaacttt atgtataggt ggcggacaag
      361 gaacagcaat attgctagaa aagtgctaga tcgattaaga aggagtgatt acatgaacaa
      421 aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc aaataataaa
      481 acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta aagggcattt
      541 aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag acagtcatct
      601 attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa ttcaccaaga
      661 tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga gtattcctta
      721 ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta
      781 tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa cactagggtt
      841 gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca
      901 tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc
      961 agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg
     1021 tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt
     1081 aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg
     1141 gaggaaataa agcggccgct gtatccatat gaccatgatt acgaattcga gctcggtacc
     1201 cggggatcct ctagagtcga cgtcacgcgt ccatggagat ctcgaggcct gcagacatgc
     1261 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1321 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1381 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct agcataaaaa
     1441 taagaagcct gcatttgcag gcttcttatt tttatggcgc gccgcattca cttcttttct
     1501 atataaatat gagcgaagcg aataagcgtc ggaaaagcag caaaaagttt cctttttgct
     1561 gttggagcat gggggttcag ggggtgcagt atctgacgtc aatgccgagc gaaagcgagc
     1621 cgaagggtag catttacgtt agataacccc ctgatatgct ccgacgcttt atatagaaaa
     1681 gaagattcaa ctaggtaaaa tcttaatata ggttgagatg ataaggttta taaggaattt
     1741 gtttgttcta atttttcact cattttgttc taatttcttt taacaaatgt tctttttttt
     1801 ttagaacagt tatgatatag ttagaatagt ttaaaataag gagtgagaaa aagatgaaag
     1861 aaagatatgg aacagtctat aaaggctctc agaggctcat agacgaagaa agtggagaag
     1921 tcatagaggt agacaagtta taccgtaaac aaacgtctgg taacttcgta aaggcatata
     1981 tagtgcaatt aataagtatg ttagatatga ttggcggaaa aaaacttaaa atcgttaact
     2041 atatcctaga taatgtccac ttaagtaaca atacaatgat agctacaaca agagaaatag
     2101 caaaagctac aggaacaagt ctacaaacag taataacaac acttaaaatc ttagaagaag
     2161 gaaatattat aaaaagaaaa actggagtat taatgttaaa ccctgaacta ctaatgagag
     2221 gcgacgacca aaaacaaaaa tacctcttac tcgaatttgg gaactttgag caagaggcaa
     2281 atgaaataga ttgacctccc aataacacca cgtagttatt gggaggtcaa tctatgaaat
     2341 gcgattaagg gccggccagt gggcaagttg aaaaattcac aaaaatgtgg tataatatct
     2401 ttgttcatta gagcgataaa cttgaatttg agagggaact tagatggtat ttgaaaaaat
     2461 tgataaaaat agttggaaca gaaaagagta ttttgaccac tactttgcaa gtgtaccttg
     2521 tacctacagc atgaccgtta aagtggatat cacacaaata aaggaaaagg gaatgaaact
     2581 atatcctgca atgctttatt atattgcaat gattgtaaac cgccattcag agtttaggac
     2641 ggcaatcaat caagatggtg aattggggat atatgatgag atgataccaa gctatacaat
     2701 atttcacaat gatactgaaa cattttccag cctttggact gagtgtaagt ctgactttaa
     2761 atcattttta gcagattatg aaagtgatac gcaacggtat ggaaacaatc atagaatgga
     2821 aggaaagcca aatgctccgg aaaacatttt taatgtatct atgataccgt ggtcaacctt
     2881 cgatggcttt aatctgaatt tgcagaaagg atatgattat ttgattccta tttttactat
     2941 ggggaaatat tataaagaag ataacaaaat tatacttcct ttggcaattc aagttcatca
     3001 cgcagtatgt gacggatttc acatttgccg ttttgtaaac gaattgcagg aattgataaa
     3061 tagttaactt caggtttgtc tgtaactaaa aacaagtatt taagcaaaaa catcgtagaa
     3121 atacggtgtt ttttgttacc ctaagtttaa actccttttt gataatctca tgaccaaaat
     3181 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3241 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3301 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3361 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3421 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3481 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3541 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3601 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3661 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3721 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3781 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3841 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3901 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3961 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4021 aatacgcagg gccc
//
