LOCUS       V004444                 3529 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004444
VERSION     V004444
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Ng YK, Cartman ST, Winzer K, Minton
            NP.
  TITLE     Integration of DNA into bacterial chromosomes from plasmids without
            a counter-selection marker
  JOURNAL   Nucleic Acids Res. 40 (8), E59 (2012)
   PUBMED   22259038
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Heap JT, Ehsaan M, Cooksley CM, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) School of Molecular Medical Sciences, The
            University of Nottingham, Centre for Biomolecular Sciences,
            University Park, Nottingham, Nottinghamshire NG7 2RD, United Kingdom
REFERENCE   3  (bases 1 to 3529)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3529)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2012"; volume: "40"; issue: "8"; pages: "E59"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JAN-2011) School of Molecular Medical Sciences, The University
            of Nottingham, Centre for Biomolecular Sciences, University Park,
            Nottingham, Nottinghamshire NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            9..646
                     /gene="pyrE fragment"
                     /label="pyrE fragment"
     misc_feature    9..308
                     /gene="pyrE fragment"
                     /note="mediates homologous recombination with the
                     chromosome of Clostridium acetobutylicum ATCC824"
     primer_bind     complement(765..781)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      929..970
                     /label="derived from fdx gene of Clostridium pasteurianum"
                     /note="derived from fdx gene of Clostridium pasteurianum"
                     /regulatory_class="terminator"
     CDS             1349..1789
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="AFA34942.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            1349..1789
                     /gene="repL"
                     /label="repL"
     CDS             1939..2559
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      2739..3327
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggag agtaatgtac ttacctttgg ggatttcata actaaaagcg gcagaagaac
       61 accatttttt ataaatacag gtaactacaa gacaggtaat caattaaata agttggctaa
      121 gttttatgct aaagcaatat atgataattt tggagatgat tttgatattt tatttgggcc
      181 tgcatataaa ggaatacctt taagtgtttc agtagctatg gcacttgata atatttatgg
      241 aattaatgca gcttattgtt caaatagaaa agaagttaaa gatcacggtg ataagggaat
      301 acttcttgga gcaaagcttg aagaaggaga cagagttata attgtagaag atgtcacaac
      361 agctggtaca tcagtatacg aaacaatgcc tatacttaaa tcacaggctg aggttgatgt
      421 aaagggaatc ataatatcag tggatagaat ggaaagaggt aagggagata agagtgcctt
      481 aactgaactt aaagaaaagt ttggatttaa aacatgttct attgttacta tggaagaggt
      541 agtagaatat ttgtataaga aaaatatcaa tggcaaagta atcatagatg ataaaatgaa
      601 agatagaatt aatgagtact ataaagagta tggagtaaaa tagtaagcgg ccgctgtatc
      661 catatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacgtca
      721 cgcgtccatg gagatctcga ggcctgcaga catgcaagct tggcactggc cgtcgtttta
      781 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      841 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      901 cgcagcctga atggcgaatg gcgctagcat aaaaataaga agcctgcatt tgcaggcttc
      961 ttatttttat ggcgcgccgc attcacttct tttctatata aatatgagcg aagcgaataa
     1021 gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg ttcagggggt
     1081 gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt acgttagata
     1141 accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg taaaatctta
     1201 atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt tcactcattt
     1261 tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga tatagttaga
     1321 atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag tctataaagg
     1381 ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca agttataccg
     1441 taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa gtatgttaga
     1501 tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg tccacttaag
     1561 taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa caagtctaca
     1621 aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa gaaaaactgg
     1681 agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac aaaaatacct
     1741 cttactcgaa tttgggaact ttgagcaaga ggcaaatgaa atagattgac ctcccaataa
     1801 caccacgtag ttattgggag gtcaatctat gaaatgcgat taagggccgg ccagtgggca
     1861 agttgaaaaa ttcacaaaaa tgtggtataa tatctttgtt cattagagcg ataaacttga
     1921 atttgagagg gaacttagat ggtatttgaa aaaattgata aaaatagttg gaacagaaaa
     1981 gagtattttg accactactt tgcaagtgta ccttgtacct acagcatgac cgttaaagtg
     2041 gatatcacac aaataaagga aaagggaatg aaactatatc ctgcaatgct ttattatatt
     2101 gcaatgattg taaaccgcca ttcagagttt aggacggcaa tcaatcaaga tggtgaattg
     2161 gggatatatg atgagatgat accaagctat acaatatttc acaatgatac tgaaacattt
     2221 tccagccttt ggactgagtg taagtctgac tttaaatcat ttttagcaga ttatgaaagt
     2281 gatacgcaac ggtatggaaa caatcataga atggaaggaa agccaaatgc tccggaaaac
     2341 atttttaatg tatctatgat accgtggtca accttcgatg gctttaatct gaatttgcag
     2401 aaaggatatg attatttgat tcctattttt actatgggga aatattataa agaagataac
     2461 aaaattatac ttcctttggc aattcaagtt catcacgcag tatgtgacgg atttcacatt
     2521 tgccgttttg taaacgaatt gcaggaattg ataaatagtt aacttcaggt ttgtctgtaa
     2581 ctaaaaacaa gtatttaagc aaaaacatcg tagaaatacg gtgttttttg ttaccctaag
     2641 tttaaactcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2701 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2761 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2821 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2881 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2941 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3001 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3061 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcagggccc
//
