LOCUS       V015800                 6373 bp    DNA     circular SYN 16-JAN-2020
DEFINITION  Exported.
ACCESSION   V015800
VERSION     V015800
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Pavlidis I, Spiller OB, Sammut Demarco G, MacPherson H, Howie SEM.,
            Norman JE, Stock SJ.
  TITLE     Cervical epithelial damage promotes Ureaplasma parvum ascending
            infection, intrauterine inflammation and preterm birth induction in
            mice
  JOURNAL   Nat Commun 11 (1), 199 (2020)
   PUBMED   31924800
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Spiller OB.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2019) Department of Medical Microbiology, Division
            of Infection and Immunity, Cardiff University, School of Medicine,
            6th Floor University Hospital, Cardiff, Wales CF14 4XN, United
            Kingdom
REFERENCE   3  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.9.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "199"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-AUG-2019) Department of Medical Microbiology, Division of
            Infection and Immunity, Cardiff University, School of Medicine, 6th
            Floor University Hospital, Cardiff, Wales CF14 4XN, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..6373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    144..160
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..184
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             424..936
                     /label="Nluc"
                     /note="NanoLuc(R) luciferase"
     CDS             1128..1640
                     /label="Nluc"
                     /note="NanoLuc(R) luciferase"
     CDS             2589..4025
                     /gene="aacA-aphD"
                     /label="Bifunctional AAC/APH"
                     /note="Bifunctional AAC/APH from Staphylococcus epidermidis
                     (strain ATCC 35984 / RP62A). Accession#: Q5HMP3"
     rep_origin      4120..4708
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gataaagtcc gtataattgt gtaaaaaccc atagctttgg acacacacta gtacggatcc
       61 acccgcaatt actgtgagtt agctcactca ttaggcaccc caggctttac actttatact
      121 tccggctcgt atattgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      181 tgaccttgat tacggaattc acggccgggg gggccacccc accaattgac gcggccgcaa
      241 ctctagaact agttgaagaa gtaatggtag cacgtgcaaa aaaataatta ttttaacaat
      301 taacaataaa gtatttaatt aataatctaa aaagttaaaa tgttataatt ataaggtata
      361 atacctttta ttatgtattc aaaaattatt tagaaaataa attaattttt aaggagattt
      421 aaaatggttt tcacattaga agatttcgtt ggtgattggc ggcaaacagc tggttacaac
      481 ttagatcaag ttttagaaca aggtggtgtt tcatcattat tccaaaactt aggtgtttca
      541 gttacaccaa ttcaacgtat tgttttatca ggtgaaaacg gtttaaaaat tgatattcat
      601 gttattattc catacgaagg tttatcaggt gatcaaatgg gtcaaattga aaaaattttc
      661 aaagttgttt acccagttga tgatcatcat ttcaaagtta ttttacatta cggtacatta
      721 gttattgatg gtgttacacc aaacatgatt gattacttcg gtcgtccata cgaaggtatt
      781 gctgttttcg atggtaaaaa aattacagtt acaggtacat tatggaacgg taacaaaatt
      841 attgatgaac gtttaattaa cccagatggt tcattattat tccgtgttac aattaacggt
      901 gttacaggtt ggcgtttatg tgaacgtatt ttagcttaaa ctagttctag aactagttga
      961 agaagtaatg gtagcacgtg caaaaaaata attattttaa caattaacaa taaagtattt
     1021 aattaataat ctaaaaagtt aaaatgttat aattataagg tataatacct tttattatgt
     1081 attcaaaaat tatttagaaa ataaattaat ttttaaggag atttaaaatg gttttcacat
     1141 tagaagattt cgttggtgat tggcggcaaa cagctggtta caacttagat caagttttag
     1201 aacaaggtgg tgtttcatca ttattccaaa acttaggtgt ttcagttaca ccaattcaac
     1261 gtattgtttt atcaggtgaa aacggtttaa aaattgatat tcatgttatt attccatacg
     1321 aaggtttatc aggtgatcaa atgggtcaaa ttgaaaaaat tttcaaagtt gtttacccag
     1381 ttgatgatca tcatttcaaa gttattttac attacggtac attagttatt gatggtgtta
     1441 caccaaacat gattgattac ttcggtcgtc catacgaagg tattgctgtt ttcgatggta
     1501 aaaaaattac agttacaggt acattatgga acggtaacaa aattattgat gaacgtttaa
     1561 ttaacccaga tggttcatta ttattccgtg ttacaattaa cggtgttaca ggttggcgtt
     1621 tatgtgaacg tattttagct taaactagtt ctagaggatt catcggccgt cgttttacaa
     1681 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     1741 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     1801 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
     1861 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
     1921 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
     1981 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
     2041 tggctacagg aaggccagac gcgaattatt tttgatggct agaagcttta ggatgaatgg
     2101 atttattctt caagaaaata catcaatttt gataagtaga aatggtaaaa acattgtata
     2161 gcattttaca caggagtctg gacttgactg agtttatgga agaagtttta attgatgata
     2221 atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt tcatttgggt
     2281 ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca cttttaagac
     2341 ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac tatcaagaca
     2401 aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag attggttgtt
     2461 ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta tatgaaaaat
     2521 taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat gaaaaaggtg
     2581 ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata gatgatgatt
     2641 ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat ggtggtagag
     2701 ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg gaagatgaag
     2761 tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa atatataaaa
     2821 tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata gtctatggta
     2881 tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca agatatatta
     2941 aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt ttagaccctc
     3001 ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga attattgaag
     3061 atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta atggaatata
     3121 gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag cattactttg
     3181 ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt gtggcatatt
     3241 tagttaataa tgaatacatt tttaaaacaa aatttagtac taataagaaa aaaggttatg
     3301 caaaagaaaa agcaatatat aattttttaa atacaaattt agaaactaat gtaaaaattc
     3361 ctaatattga atattcgtat attagtgatg aattatctat actaggttat aaagaaatta
     3421 aaggaacttt tttaacacca gaaatttatt ctactatgtc agaagaagaa caaaatttgt
     3481 taaaacgaga tattgccagt tttttaagac aaatgcacgg tttagattat acagatatta
     3541 gtgaatgtac tattgataat aaacaaaatg tattagaaga gtatatattg ttgcgtgaaa
     3601 ctatttataa tgatttaact gatatagaaa aagattatat agaaagtttt atggaaagac
     3661 taaatgcaac aacagttttt gagggtaaaa agtgtttatg ccataatgat tttagttgta
     3721 atcatctatt gttagatggc aataatagat taactggaat aattgatttt ggagattctg
     3781 gaattataga tgaatattgt gattttatat acttacttga agatagtgaa gaagaaatag
     3841 gaacaaattt tggagaagat atattaagaa tgtatggaaa tatagatatt gagaaagcaa
     3901 aagaatatca agatatagtt gaagaatatt atcctattga aactattgtt tatggaatta
     3961 aaaatattaa acaggaattt atcgaaaatg gtagaaaaga aatttataaa aggacttata
     4021 aagattaata aagatctacg aaggcatgac caaaatccct taacgtgagt tttcgttcca
     4081 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4141 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4201 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4261 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4321 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4381 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4441 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4501 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4561 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4621 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4681 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4741 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4801 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4861 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4921 gcgttggccg attcattaat gcacgctagc atttaaatgg taccctttta cacaattata
     4981 cggactttat cctgcagggg cccaattgtg taaaagtaaa aaggccatat aacagtcctt
     5041 ttacggtaca atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag
     5101 tacattttac actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg
     5161 acgtttctaa aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag
     5221 aatatattca agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct
     5281 attatgagcg cagctttacg acacgtgtag gcacgctaga attaaaagta cccagaacac
     5341 gtgatggcca tttttcaccc acagtgtttg aacgttatca acgaaacgaa aaagccctca
     5401 tggcttcaat gttggaaatg tatgtatcag gcgtttcaac tcgtaaagta tcaaaaattg
     5461 tggaagaact ttgtggtaaa tccgtctcta agtccttcgt ttctagctta acagaacagc
     5521 tagaacctat ggttaacgag tgacagaatc gtttattatc agaaaaaaat tatccttact
     5581 taatgaccga tgtactctat ataaaagtac gagaagaaaa tcgagtactc tcaaaaagct
     5641 gtcatatagc gattggaata accaaagatg gcgaccgtga aattatcggc ttcatgattc
     5701 aaagtggcga aagcgaagag acctggacaa cattttttga atacctaaaa gaacgcggtt
     5761 tacaaggtac ggaactcgtt atttctgatg cgcacaaagg attagtctct gccattagaa
     5821 aatccttcac caacgtaagt tggcaaagat gccaagttca cttcctaaga aatatcttta
     5881 ccaccattcc taaaaaaaat tcaaaatctt tcagagaagc tgttaaagga atttttaagt
     5941 tcacagatat taacttagcg cgtgaggcta aaaatcgatt gattcatgat tatatcgatc
     6001 aaccaaaata ttcaaaagct tgcgcatcat tggatgatgg attcgaagac gcctttcaat
     6061 ataccgtaca aggaaattcc cacaatcgac taaagagtac caatctaatt gaacgactga
     6121 atcaagaagt acgcagaaga gaaaagatta ttcgcatctt ccccaatcaa acatcagcca
     6181 atcgcttaat tggagccgtt cttatggacc tacatgatga atggatttat tcttcaagaa
     6241 aatacatcaa ttttgataag tagaaatggt aaaaacattg tatagcattt tacacaggag
     6301 tctggacttg actcacttcc tttattattt ttcatttttt tgacctcgag ggggggccca
     6361 ccatacagct gac
//
