LOCUS       62056_17525        9402 bp DNA     circular SYN 19-MAY-2020
DEFINITION  Cloning vector pMT469, complete sequence.
ACCESSION   MN991286
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Taketani M, Zhang J, Zhang S, Triassi AJ, Huang YJ, Griffith LG, 
            Voigt CA.
  TITLE     Genetic circuit design automation for the gut resident species 
            Bacteroides thetaiotaomicron
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32231334
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Taketani M, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2020) Biological Engineering, MIT, Synthetic 
            Biology Center 500 Technology Square NE47-140, Cambridge, MA 02139, 
            USA
REFERENCE   3  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-2020) Biological Engineering, MIT, Synthetic Biology Center 
            500 Technology Square NE47-140, Cambridge, MA 02139, USA"
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1378..2235
                     /label=AmpR
                     /note="beta-lactamase"
     oriT            complement(2369..2478)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2649..3037
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      3078..3107
                     /label=tL17
                     /note="tL17"
                     /regulatory_class="terminator"
     regulatory      3123..3130
                     /label=L3S3P21
                     /note="L3S3P21"
                     /regulatory_class="terminator"
     misc_feature    3134..3137
                     /label=Ascar
                     /note="Ascar"
     protein_bind    3288..3306
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        3331..3406
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      3414..3474
                     /label=L3S3P41
                     /note="L3S3P41"
                     /regulatory_class="terminator"
     misc_feature    3475..3478
                     /label=Bscar
                     /note="Bscar"
     protein_bind    complement(3567..3585)
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    3652..3754
                     /label=M3
                     /note="M3"
     regulatory      3755..3825
                     /label=ECK120034435
                     /note="ECK120034435"
                     /regulatory_class="terminator"
     misc_feature    3826..3829
                     /label=Dscar
                     /note="Dscar"
     regulatory      3830..3937
                     /label=PM1
                     /note="PM1"
                     /regulatory_class="promoter"
     misc_feature    3938..4040
                     /label=M4
                     /note="M4"
     regulatory      4041..4107
                     /label=ECK120033736
                     /note="ECK120033736"
                     /regulatory_class="terminator"
     misc_feature    4108..4111
                     /label=Escar
                     /note="Escar"
     regulatory      4112..4219
                     /label=PM1
                     /note="PM1"
                     /regulatory_class="promoter"
     misc_feature    4220..4322
                     /label=M3
                     /note="M3"
     regulatory      4323..4393
                     /label=ECK120034435
                     /note="ECK120034435"
                     /regulatory_class="terminator"
     misc_feature    4394..4397
                     /label=Fscar
                     /note="Fscar"
     regulatory      4398..4513
                     /label=P_BA
                     /note="P_BA"
                     /regulatory_class="promoter"
     misc_feature    4514..4616
                     /label=M4
                     /note="M4"
     regulatory      4617..4683
                     /label=ECK120033736
                     /note="ECK120033736"
                     /regulatory_class="terminator"
     misc_feature    4684..4687
                     /label=scar
                     /note="nonstandard type: scar"
     regulatory      4688..4803
                     /label=P_BA
                     /note="P_BA"
                     /regulatory_class="promoter"
     misc_feature    4804..4906
                     /label=M1
                     /note="M1"
     regulatory      4907..4967
                     /label=L3S3P41
                     /note="L3S3P41"
                     /regulatory_class="terminator"
     misc_feature    4968..4971
                     /label=Cscar
                     /note="Cscar"
     misc_feature    4972..4975
                     /label=Ascar
                     /note="Ascar"
     regulatory      4976..5083
                     /label=PM4
                     /note="PM4"
                     /regulatory_class="promoter"
     misc_RNA        5085..5135
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      5159..5201
                     /label=rpiL*
                     /note="rpiL*"
                     /regulatory_class="terminator"
     misc_feature    5202..5207
                     /label=similar to luciferase reporter
                     /note="similar to luciferase reporter"
     regulatory      5215..5285
                     /label=L3S2P56
                     /note="L3S2P56"
                     /regulatory_class="terminator"
     misc_feature    5293..5296
                     /label=Bscar
                     /note="Bscar"
     regulatory      5297..5404
                     /label=PM1
                     /note="PM1"
                     /regulatory_class="promoter"
     misc_feature    5405..5479
                     /label=RiboJ00
                     /note="RiboJ00"
     regulatory      5480..5522
                     /label=rpiL*
                     /note="rpiL*"
                     /regulatory_class="terminator"
     CDS             5523..6035
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     regulatory      6046..6116
                     /label=L3S2P56
                     /note="L3S2P56"
                     /regulatory_class="terminator"
     misc_feature    6124..6127
                     /label=Dscar
                     /note="Dscar"
     regulatory      6128..6235
                     /label=PM3
                     /note="PM3"
                     /regulatory_class="promoter"
     misc_feature    6236..6310
                     /label=RiboJ00
                     /note="RiboJ00"
     regulatory      6311..6353
                     /label=rpiL*
                     /note="rpiL*"
                     /regulatory_class="terminator"
     misc_feature    6354..6359
                     /label=similar to luciferase reporter
                     /note="similar to luciferase reporter"
     regulatory      6367..6437
                     /label=L3S2P56
                     /note="L3S2P56"
                     /regulatory_class="terminator"
     misc_feature    6445..6448
                     /label=Cscar
                     /note="Cscar"
     regulatory      6449..6731
                     /label=BT1311
                     /note="BT1311"
                     /regulatory_class="promoter"
     regulatory      6692..6695
                     /label=-33 sigma factor recognitions site
                     /note="-33 sigma factor recognitions site"
                     /regulatory_class="other"
     regulatory      6718..6725
                     /label=-7 sigma factor recognitions site
                     /note="-7 sigma factor recognitions site"
                     /regulatory_class="other"
     CDS             6758..7369
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /label=TetR/AcrR family transcriptional regulator
                     /note="VFA0359-Bt"
                     /protein_id="QJT41694.1"
                     /translation="MQKKLTRSQQKHLDIINAAKEEFIEFGFLAANMDRITSSAEVSKR
                     TLYRHFESKEVLFESVLTIINDSVNESISYHFDPNKSTEEQLTEIAYKEIDVLYKTYGI
                     ALARTIVMEFLRQPEMAKTLIQNIYSIRAITQWFRSAIEAKRLKDADPKLMTDVYVSLF
                     QGLFFWPQVMHLDLEPHGEELSQKIETLTTIFLQSYGVAE"
     regulatory      7386..7446
                     /label=L3S2P21
                     /note="L3S2P21"
                     /regulatory_class="terminator"
     primer_bind     complement(7572..7592)
                     /label=pNBU1_seq.REV
                     /note="pNBU1_seq.REV"
     misc_recomb     7690..8019
                     /label=attN1 region
                     /note="attN1 region"
     misc_feature    7817..7830
                     /label=CC Region
                     /note="CC Region"
     misc_difference 7829
                     /label=mutation for higher integration efficiency
                     /note="mutation for higher integration efficiency"
     CDS             8035..9372
                     /codon_start=1
                     /transl_table=11
                     /product="IntN1"
                     /label=IntN1
                     /note="NBU1 integrase"
                     /protein_id="QJT41695.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNFRVVKRALLRYELYVRATKRGQK
                     GFILDVDLVTPDTLRDMWDFFQNEYQYYELYPSIYEAIPEKRTPQPRSKNTLIDCFSRI
                     RTFFLWCFDNKRTTNRPFDKFPIEECTYGTPYYITLEERDRIFNADLSATPQLAIQRDI
                     FIFQTLIGCRVSDLYRMTKLNVVNEAIEYIPKKTKEGNPVTVRVPLNDKAKEILERYKE
                     YEGKLLPFISEQKYNDAIKKIFKLAGVDRIVTILDPLTHNEIKRPIYEVASSHLARRTF
                     IGNIYKKVKDPNLVSALSGHKEGSKAFRRYRDIDEEMKKDLVKLLD"
ORIGIN
        1 agagtagcga tattaaaaga atccgatgag aaaagactaa tatttatcta tccattcagt
       61 ttgatttttc aggactttac atcgtcctga aagtatttgt tggtaccggt accgaggacg
      121 cgtaaacatt tacagttgca tgtggcctat tgtttttagc cgttaaatat tttataacta
      181 ttaaatagcg atacaaattg ttcgaaacta atattgttta tatcatatat tctcgcatgt
      241 tttaaagctt tattaaattg attttttgta aacagttttt cgtactcttt gttaacccat
      301 ttcattacaa aagtttcata tttttttctc tctttaaatg ccatttttgc tggctttctt
      361 tttaatacaa ttaatgtgct atccacttta ggttttggat ggaaataata cctaggaatt
      421 tttgctaata tagaaatatc tacctctgcc attaacagca atgctagtga tctgtttgta
      481 tctaataaca ttttagcaaa accatattcc actattaaat aacttattgt ggctgaactt
      541 tcaaaaacaa tttttcgaat tatatttgtg cttatgttgt aaggtatgct gccaaatatt
      601 ttatatggat tgtggctagg aaatgtaaat ttcagtatat catcatttac tatttgatag
      661 ttaggataat ttaagagctt attacgagtt acctcacata atttagaatc aatttctatc
      721 gccgttacaa aattacatct ctttaccaat ccagcagtaa aatgaccttt ccctgcacct
      781 atttcaaaga tgttatcttt ttcatctaaa cttatgcaat tcattatttt ttctatgtga
      841 tattttgaag taataaaatt ttgactatct tttatattta ctttgttcat tataacctct
      901 ccttaattta ttgcatctct tttcgaatat ttatgttttt tgagaaaaga acgtactcat
      961 ggttcatccc gatatgcgta tcggtctgta tatcagcaac tttctatgtg tttcaactac
     1021 aatagtcatc tattctcatc tttctgagtc caccccctgc aaagcccctc tttacgacat
     1081 aaaaattcgg tcggaaaagg tatgcaaaag atgtttctct ctttaagaga aactcttcgg
     1141 gatgcaaaaa tatgaaaata actccaattc accaaattat atagcgactt ttttacaaaa
     1201 tgctaaaatt tgttgatttc cgtcaagcaa ttgttgagca aaaatgtctt ttacgataaa
     1261 atgatacctc aatatcaact gtttagcaaa acgatatttc tcttaaagag agaaacacct
     1321 ttttgttcac caatccccga cttttaatcc cgcggccatg attgaaaaag gaagagtatg
     1381 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1441 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1501 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1561 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1621 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1681 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1741 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1801 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1861 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1921 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1981 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2041 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcacgc
     2101 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2161 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2221 ctgattaagc attggtaact gtcagaccaa gtttactcat aacgcgtcaa ttcgaggggg
     2281 atcaattccg tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcagggtta tgcagcggaa aacggaattg atccggccac gatgcgtccg
     2641 gcgtagagga tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg
     2701 tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc
     2761 ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac
     2821 taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag
     2881 gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa
     2941 cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag
     3001 caattttgag tgacacagga acacttaacg gctgacatgg gaattcccct ccaccgcggt
     3061 gtattaatgc ggctgcaatt tttttgggcg gggccgccca aaaaaatcct agcaccctgc
     3121 agggtctccc gctggagttt tgcacccgct ttccaagaga agaaagcctt gttaaattga
     3181 cttagtgtaa aagcgcagta ctgcttgacc ataagaacaa aaaaatctct atcactgata
     3241 gggataaagt ttggaagata aagctaaaag ttcttatctt tgcagtctcc ctatcagtga
     3301 tagagacgca caaagataat agaacttaag gttttagagc tagaaatagc aagttaaaat
     3361 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttggatcca
     3421 aaaaaaaaaa acaccctaac gggtgttttt ttttttttgg tctgccgcgg ccgctacgtt
     3481 ttgcacccgc tttccaagag aagaaagcct tgttaaattg acttagtgta aaagcgcagt
     3541 actgcttgac cataagaaca aaaaaatctc tatcactgat agggataaag tttggaagat
     3601 aaagctaaaa gttcttatct ttgcagtctc cctatcagtg atagagacgc acaaagataa
     3661 ttaagttaag ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3721 cttgaaaaag tggcaccgag tcggtgcttt ttttggatcc ctcggtacca aattccagaa
     3781 aagagacgct gaaaagcgtc ttttttcgtt ttggtccgcg gccgcaggtt gctgtcatag
     3841 gccggttgtc cggtagtccg gatgatttaa ggaagatttc tcttcttttt tcactttctc
     3901 cgaacaacct ccacttaagt tctattatct ttgcagtcaa agatacttat gttagaagtt
     3961 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4021 accgagtcgg tgcttttttt ggatccaacg catgagaaag cccccggaag atcaccttcc
     4081 gggggctttt ttattgcgcg cggccgcgct ttgctgtcat aggccggttg tccggtagtc
     4141 cggatgattt aaggaagatt tctcttcttt tttcactttc tccgaacaac ctccacttaa
     4201 gttctattat ctttgcagtc aaagataatt aagttaaggg ttttagagct agaaatagca
     4261 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     4321 ttggatccct cggtaccaaa ttccagaaaa gagacgctga aaagcgtctt ttttcgtttt
     4381 ggtccgcggc cgccgcttta caaagaaaat tcgacaaact gttatttttc tatctattta
     4441 aatgtacacc cgtttgttta ctttacttta gttatgtacc tttgtcggca atgtacaccc
     4501 gaaagtttac tttcaaagat acttatgtta gaagttttag agctagaaat agcaagttaa
     4561 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat
     4621 ccaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcgcggc
     4681 cgctgtctta caaagaaaat tcgacaaact gttatttttc tatctattta aatgtacacc
     4741 cgtttgttta ctttacttta gttatgtacc tttgtcggca atgtacaccc gaaagtttac
     4801 tttcaaagat aatagaactt aaggttttag agctagaaat agcaagttaa aataaggcta
     4861 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggat ccaaaaaaaa
     4921 aaaacaccct aacgggtgtt tttttttttt tggtctgccg cggccgcaat gggagtgctg
     4981 tcataggccg gttgtccggt agtccggatg atttaaggaa gatttctctt cttttttcac
     5041 tttctccgaa caacctccat tctaacataa gtatctttgc agtagctgtc accggatgtg
     5101 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaacg
     5161 cattttaaaa taaaataaat tatttattta attaaacgaa tatgtaatga actgggatcc
     5221 ctcggtacca aattttcgaa aaaagacgct gaaaagcgtc ttttttcgtt ttggtccgcg
     5281 gccgccggcc gctacgtgct gtcataggcc ggttgtccgg tagtccggat gatttaagga
     5341 agatttctct tcttttttca ctttctccga acaacctcca cttaagttct attatctttg
     5401 cagtagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
     5461 tacaaataat tttgtttaac gcattttaaa ataaaataaa ttatttattt aattaaacga
     5521 atatggtttt tactctggaa gattttgttg gcgattggcg tcagaccgcg ggttataatt
     5581 tggatcaagt cctggaacag ggtggcgtaa gctctctgtt ccagaacctg ggtgtgagcg
     5641 tgacgccgat tcagcgcatc gttctgtccg gcgagaacgg tctgaaaatt gatattcatg
     5701 tgatcatccc gtacgaaggc ctgagcggtg accaaatggg tcaaatcgag aaaatcttta
     5761 aagtcgtcta cccagttgac gatcaccact tcaaggttat cttgcattac ggtacgctgg
     5821 tgattgatgg tgtgaccccg aatatgattg actatttcgg ccgtccgtat gaaggcattg
     5881 ccgtttttga cggtaaaaag atcaccgtca ccggtaccct gtggaatggc aataagatta
     5941 ttgacgagcg tctgattaac ccggacggca gcctgctgtt ccgcgtgacc atcaacggtg
     6001 tcacgggttg gcgtctgtgc gagcgcatcc tggcataatg aactgggatc cctcggtacc
     6061 aaattttcga aaaaagacgc tgaaaagcgt cttttttcgt tttggtccgc ggccgccggc
     6121 cgcaggttgc tgtcataggc cggttgtccg gtagtccgga tgatttaagg aagatttctc
     6181 ttcttttttc actttctccg aacaacctcc accttaactt aattatcttt gcagtagctg
     6241 tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa
     6301 ttttgtttaa cgcattttaa aataaaataa attatttatt taattaaacg aatatgtaat
     6361 gaactgggat ccctcggtac caaattttcg aaaaaagacg ctgaaaagcg tcttttttcg
     6421 ttttggtccg cggccgccgg ccgcaatgtg atctggaaga agcaatgaaa gctgctgtta
     6481 agtctccgaa tcaggtattg ttcctgacag gtgtattccc atccggtaaa cgcggatact
     6541 ttgcagttga tctgactcag gaataaatta taaattaagg taagaagatt gtaggataag
     6601 ctaatgaaat agaaaaagga tgccgtcaca caacttgtcg gcattctttt ttgttttatt
     6661 agttgaaaat atagtgaaaa agttgcctaa atatgtatgt taacaaatta tttgtcgtaa
     6721 ctttgcactc caaatctgtt tttaacatat ggcactaatg cagaaaaaat tgactcgttc
     6781 tcagcagaaa cacttggaca tcatcaacgc tgctaaagaa gaattcatcg aattcggttt
     6841 cttggctgct aacatggacc gtatcacttc ttctgctgaa gtatctaaac gtactttgta
     6901 ccgtcacttc gaatctaaag aagtattgtt cgaatctgta ttgactatca tcaacgactc
     6961 tgtaaacgaa tctatctctt accacttcga cccgaacaaa tctactgaag aacagttgac
     7021 tgaaatcgct tacaaagaaa tcgacgtatt gtacaaaact tacggtatcg ctttggctcg
     7081 tactatcgta atggaattct tgcgtcagcc ggaaatggct aaaactttga tccagaacat
     7141 ctactctatc cgtgctatca ctcagtggtt ccgttctgct atcgaagcta aacgtttgaa
     7201 agacgctgac ccgaaattga tgactgacgt atacgtatct ttgttccagg gtttgttctt
     7261 ctggccgcag gtaatgcact tggacttgga accgcacggt gaagaattgt ctcagaaaat
     7321 cgaaactttg actactatct tcttgcagtc ttacggtgta gctgaataac aatgaatggg
     7381 ctttcctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
     7441 tggtccctaa ttgcctatct tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat
     7501 cagccttact tgtgcctgtt ctatttccga accgaccgct tgtatgaatc catcaaaatt
     7561 cgttttctct atgttggatt ccttgttgct catattgtga tgataatttc tacaaatata
     7621 gtcattggta actatctatg aaactgtttg atacttttac tcatcggatt cttttaatat
     7681 cgctactctc tacgttcaac caaaagaaat aatgacttac tgctatattt tttgcacgtg
     7741 tggggaaaat gtggggaaaa ttcaagcaaa agaaaaagct aagtattgaa ctatcaaata
     7801 cttagctttc tttcttgtac ccagaccccg catttgaaat aattaaagtg gggaaaatgt
     7861 gggtaaaaag aaaaatgcgg aaaaacgcca caattacact gtatttcaat atgttataat
     7921 cctattaaat tttaatccaa gtttaatcga attgcaaaat atttagcaga tgtggggaaa
     7981 atgctgggga aaatatttat atttgcagca gagtaaaatt caacctgaaa gtctatgaaa
     8041 gtaaccttta tcattaaaaa agcagccaaa cgatatgata cagaatccat ggctacaatc
     8101 tatgtccgtt ttagaaacgg aaggcagtta gactccgttg ctcctactca gttagccatc
     8161 aatcccaatc tatgggatga taaagacgaa tgtgtaaaaa cgaaagctgt ctgcaatgaa
     8221 gaaatgcgta cccatataaa tgaagagata cgccagttga aaacctatat cgagaaggta
     8281 tatcaacaag aaaaggaagc aatagacaaa gaatggctaa aaacaacact tgataaattt
     8341 taccatcctg aaaaatattt tttgccggag gaagtggtta tcaagcctac cattggagaa
     8401 ctattcgatg aatttctaaa caagcaccct ttgtcggaag tacgaaagaa aaatttccgg
     8461 gttgtcaaaa gagccttact gcgttatgaa ctatatgtaa gggctacaaa gagaggacaa
     8521 aagggcttta tccttgatgt ggatttggta acacctgaca cgcttcggga tatgtgggat
     8581 ttctttcaga acgaatacca gtattatgaa ctttacccga gcatttatga agccattccc
     8641 gaaaagagga caccacagcc cagaagcaaa aacacgctga tagactgttt ttcaagaata
     8701 cgcacattct tcctgtggtg cttcgataac aaacgcacca caaacagacc tttcgacaag
     8761 tttccgatag aggagtgtac atatggtaca ccttattata taacactcga agaaagggac
     8821 aggattttta atgcagacct ttctgccacc ccacaactgg caatacagag ggatatattc
     8881 atatttcaga cactgatagg atgcagggtg agcgacctgt accgaatgac caaactaaat
     8941 gtggtcaatg aagccataga atatattccc aagaaaacca aagaggggaa tccggttacg
     9001 gtacgtgttc cacttaacga caaagcgaaa gaaatccttg aacgctacaa agaatatgag
     9061 ggaaaactgt tgccgttcat atccgagcaa aagtacaatg atgccataaa aaagatattc
     9121 aaattagctg gagttgaccg catcgtaaca atcttagacc cgttgacgca caacgaaatc
     9181 aaacgaccta tttatgaagt ggcaagcagc catctggcaa gacgtacgtt tatcggcaat
     9241 atctataaaa aagtgaaaga cccgaacctt gtttccgcac tgtcgggaca caaggaggga
     9301 agcaaagctt tcagacgata cagggatatt gacgaagaaa tgaagaaaga ccttgtaaaa
     9361 ctattagact gataaaagta tcaaacagtt tcatagatag tt
//
