LOCUS       40924_32455        3694 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMT-suicide1-SacB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Lebens MR, Karlsson SL.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 3694)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-JUN-2013) Deptartment of Microbiology "
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..107
                     /label=multicloning region
                     /note="multicloning region"
     rep_origin      complement(124..512)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(546..655)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        1000..1102
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1103..1759
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2163..3581
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg atatctggat ccacgaagct
       61 tcccatggtg acgtcaccgg ttctagatac ctaggtgagc tctggtaccc tctagtcaag
      121 gcctgtcagc cgttaagtgt tcctgtgtca ctgaaaattg ctttgagagg ctctaagggc
      181 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa
      241 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg
      301 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct
      361 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg
      421 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac
      481 tatcaacagg ttgaactgct gatcttcaga tctccgcttg ccctcatctg ttacgccggc
      541 ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
      601 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
      661 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
      721 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
      781 agcggaaaag cgctgcttcc ctgctgtttt gtggaatatc taccgactgg aaacaggcaa
      841 atgcaggaaa ttactgaact gaggggacag gcgagagatc tggcctaggc cgaccgaata
      901 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      961 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
     1021 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tgtcgagatt
     1081 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1141 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1201 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1261 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1321 acgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1381 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1441 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1501 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     1561 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1621 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1681 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1741 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgcccat aaacgcctgg
     1801 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tattttttag
     1861 ttctttaggc ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa
     1921 tagaccagtt gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg
     1981 ggtttgttac tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt
     2041 ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt
     2101 caaacaggag ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa
     2161 cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc
     2221 tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa
     2281 catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa cagcaaaaaa
     2341 atgaaaaata tcaagttcct gaattcgatt cgtccacaat taaaaatatc tcttctgcaa
     2401 aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact gtcgcaaact
     2461 atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg gatgacacat
     2521 cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg aaaaacgctg
     2581 gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta aaagaccaaa
     2641 cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt ttattctaca
     2701 ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa gttaacgtat
     2761 cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca atctttgacg
     2821 gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac tacagctcag
     2881 gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac aaatacttag
     2941 tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct ttatttaaca
     3001 aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa
     3061 gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt gagctaaacg
     3121 atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca gtaacagatg
     3181 aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc actgactccc
     3241 gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg cttggttatg
     3301 tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa
     3361 tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta cctcaagcga
     3421 aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac gcagacaaac
     3481 aatcaacgtt tgcgccaagc ttcctgctga acatcaaagg caagaaaaca tctgttgtca
     3541 aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataaaaacgc aaaagaaaat
     3601 gccgattgag gccagtttgc tcaggctctc cccgtggagg taataattga cgatatgatc
     3661 agtaatacga ctcactagtg ggcagatctt cgaa
//
