LOCUS       pMT_BioEase-DEST        5375 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for inducible high-level expression of
            biotinylated fusion proteins in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    pMT BioEase-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        407..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             864..866
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             867..1082
                     /label=BioEase(TM) tag
                     /note="biotinylated sequence derived from the C-terminus of
                     the alpha-subunit of Klebsiella pneumoniae oxaloacetate 
                     decarboxylase"
     CDS             1095..1118
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     protein_bind    1128..1252
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1277..1307
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1361..2017
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2341..2643
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2687..2811)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    complement(2877..3011)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3155..3171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3179..3195)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3203..3233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3248..3269)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3557..4145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4319..5176)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5177..5281)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattaatt cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
      841 tagcgtttaa acttaagctt accatgggcg ccggcacccc ggtgaccgcc ccgctggcgg
      901 gcactatctg gaaggtgctg gccagcgaag gccagacggt ggccgcaggc gaggtgctgc
      961 tgattctgga agccatgaag atggaaaccg aaatccgcgc cgcgcaggcc gggaccgtgc
     1021 gcggtatcgc ggtgaaagcc ggcgacgcgg tggcggtcgg cgacaccctg atgaccctgg
     1081 cgggctctgg atccgatctg tacgacgatg acgataaggt acatcaaaca agtttgtaca
     1141 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     1201 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     1261 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     1321 ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1381 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1441 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1501 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1561 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1621 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1681 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1741 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1801 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1861 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1921 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     1981 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat ccggcttact
     2041 aaaagccaga taacagtatg cgtatttgcg cgctcgcgaa ccggtgtata cccgaagtat
     2101 gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     2161 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     2221 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     2281 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg gactggtgaa
     2341 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     2401 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     2461 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     2521 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     2581 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     2641 atataaatgt caggctccgt tatacacagc cagtctgcag gtcgaccata gtgactggat
     2701 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     2761 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataattaa
     2821 ttaagatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctaagatc
     2881 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2941 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     3001 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3061 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt
     3121 cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4621 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4921 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     5341 cctataaaaa taggcgtatc acgaggccct ttcgt
//
