LOCUS       pMT_BiP_V5-His_C        3638 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Drosophila vector for inducible high-level expression of secreted 
            and tagged proteins. For other reading frames, use pMT/BiP/V5-His A 
            or pMT/BiP/V5-His B.
ACCESSION   .
VERSION     .
KEYWORDS    pMT BiP V5-His C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..3638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     851..904
                     /label=BiP signal sequence
                     /note="Drosophila BiP signal sequence"
     misc_feature    905..998
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             999..1040
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1050..1067
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(1140..1274)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1418..1434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1442..1458)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1466..1496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1511..1532)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1820..2408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2582..3439)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3440..3544)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
      841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct
      901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat
      961 ccagcacagt ggcggccgct cgaggtcacc cattcgaagg taagcctatc cctaaccctc
     1021 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     1081 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     1141 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     1201 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     1261 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     1321 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     1381 agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1441 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1501 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1561 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1621 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1681 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1741 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1801 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1861 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1921 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1981 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2041 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2101 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2161 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2221 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2281 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2341 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2401 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2581 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2641 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2701 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2761 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2821 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2881 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2941 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3001 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3061 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3121 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3181 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3241 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3301 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3361 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3421 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3541 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     3601 taacctataa aaataggcgt atcacgaggc cctttcgt
//
