LOCUS       pMT_BiP_V5-His_B        3646 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Drosophila vector for inducible high-level expression of secreted 
            and tagged proteins. For other reading frames, use pMT/BiP/V5-His A 
            or pMT/BiP/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMT BiP V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..3646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     851..904
                     /label=BiP signal sequence
                     /note="Drosophila BiP signal sequence"
     misc_feature    905..1006
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1007..1048
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1058..1075
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(1148..1282)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1426..1442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1450..1466)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1474..1504)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1519..1540)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1828..2416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2590..3447)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3448..3552)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
      841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct
      901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat
      961 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     1021 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     1081 ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc
     1141 gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1201 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     1261 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     1321 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     1381 ttatgatcag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc
     1441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     1501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     1561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     2281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3601 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
