LOCUS       V015674                 7014 bp    DNA     circular SYN 10-JUL-2020
DEFINITION  Exported.
ACCESSION   V015674
VERSION     V015674
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2020) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (17-FEB-2020) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
FEATURES             Location/Qualifiers
     source          1..7014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   29..172
                     /rpt_family="response_element"
                     /rpt_unit_range=29 .. 52
                     /rpt_unit_seq="cacacgtgggttcccgcacgtccg"
     regulatory      29..172
                     /label="sextuplicate hypoxia-response element"
                     /note="sextuplicate hypoxia-response element"
                     /regulatory_class="response_element"
     protein_bind    213..240
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      271..288
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     289..393
                     /note="tPA prepro (secretory signal peptide)"
     CDS             409..1689
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             1717..2337
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2373..2959
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2960..3676
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3802..3923
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3930..4385)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4412..4516
                     /label="AmpR promoter"
     promoter        4518..4875
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4910..5701
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5936..5983
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6312..6900
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgagcggca cacgtgggtt cccgcacgtc cgcacacgtg
       61 ggttcccgca cgtccgcaca cgtgggttcc cgcacgtccg cacacgtggg ttcccgcacg
      121 tccgcacacg tgggttcccg cacgtccgca cacgtgggtt cccgcacgtc cgccggaatt
      181 tcgacgtcgg ccatagcggc cgcggaattg aaaacaaaca gacaatctgg tctgtttgta
      241 ttataagtaa ggactagtcc atcgatcgat cccgccgcca ccatggcgat ggatgcaatg
      301 aagagagggc tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc gcccagccag
      361 gaaatccatg cccgattcag aagaggagcc agattggcgc gccaaatggt gtcgaataac
      421 gctttagaaa caattattaa cgcccggtta ccaggcgaag aggggctgtg gcagattcat
      481 ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg gcgtgatgcc cataactgaa
      541 aacagcctgg atgccgaaca aggtttagtt ataccgccgt ttgtggagcc acatattcac
      601 ctggacacca cgcaaaccgc cggacaaccg aactggaatc agtccggcac gctgtttgaa
      661 ggcattgaac gctgggccga gcgcaaagcg ttattaaccc atgacgatgt gaaacaacgc
      721 gcatggcaaa cgctgaaatg gcagattgcc aacggcattc agcatgtgcg tacccatgtc
      781 gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc tggaagtgaa gcaggaagtc
      841 gcgccgtgga ttgatctgca aatcgtcgcc ttccctcagg aagggatttt gtcgtatccc
      901 aacggtgaag cgttgctgga agaggcgtta cgcttagggg cagatgtagt gggggcgatt
      961 ccgcattttg aatttacccg tgaatacggc gtggagtcgc tgcataaaac cttcgccctg
     1021 gcgcaaaaat acgaccgtct catcgacgtt cactgtgatg agatcgatga cgagcagtcg
     1081 cgctttgtcg aaaccgttgc tgccctggcg caccatgaag gcatgggcgc gcgagtcacc
     1141 gccagccaca ccacggcaat gcactcctat aacggggcgt atacctcacg cctgttccgc
     1201 ttgctgaaaa tgtccggtat taactttgtc gccaacccgc tggtcaatat tcatctgcaa
     1261 ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc gcgttaaaga gatgctggag
     1321 tccggcatta acgtctgctt tggtcacgat gatgtcttcg atccgtggta tccgctggga
     1381 acggcgaata tgctgcaagt gctgcatatg gggctgcatg tttgccagtt gatgggctac
     1441 gggcagatta acgatggcct gaatttaatc acccaccaca gcgcaaggac gttgaatttg
     1501 caggattacg gcattgccgc cggaaacagc gccaacctga ttatcctgcc ggctgaaaat
     1561 gggtttgatg cgctgcgccg tcaggttccg gtacgttatt cggtacgtgg cggcaaggtg
     1621 attgccagca cacaaccggc acaaaccacc gtatatctgg agcagccaga agccatcgat
     1681 tacaaacgta tcagcggagc taatggcgtc atggctaaga tcgtggaagt caaacaccca
     1741 ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa gcgctttcgc
     1801 gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga cctcgaaacg
     1861 gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat caaaggtaag
     1921 aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg tgtgctggaa
     1981 aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag
     2041 ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc gctgatcgtt
     2101 gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg
     2161 ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat cgctgcgctg
     2221 gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgatcaggg actgaacgag
     2281 cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg tacgaaataa
     2341 gcttgtcgaa attcgctagc cgcgcggatc cgcccctctc cctccccccc ccctaacgtt
     2401 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     2461 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     2521 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     2581 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     2641 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     2701 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     2761 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     2821 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     2881 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     2941 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3001 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3061 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3121 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3181 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3241 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3301 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3361 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3421 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3481 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3541 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3601 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3661 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     3721 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3781 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3841 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3901 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     3961 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4021 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4081 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4141 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     4201 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4261 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4321 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4381 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4441 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4501 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4561 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4621 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4681 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4741 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4801 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4861 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4921 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4981 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5041 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     5101 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5161 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5221 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5281 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5341 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5401 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     5461 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5521 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5581 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5641 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5701 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5761 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5821 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5881 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     5941 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     6001 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     6061 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     6121 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     6181 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6241 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6301 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6361 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6421 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6481 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6541 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6661 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6721 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6781 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6841 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6901 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6961 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
