LOCUS       V015841                 7230 bp    DNA     circular SYN 10-JUL-2020
DEFINITION  Exported.
ACCESSION   V015841
VERSION     V015841
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2020) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (17-FEB-2020) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
FEATURES             Location/Qualifiers
     source          1..7230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   29..172
                     /rpt_family="response_element"
                     /rpt_unit_range=29 .. 52
                     /rpt_unit_seq="cacacgtgggttcccgcacgtccg"
     regulatory      29..172
                     /label="sextuplicate hypoxia-response element"
                     /note="sextuplicate hypoxia-response element"
                     /regulatory_class="response_element"
     promoter        188..391
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    429..456
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      487..504
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     505..609
                     /note="tPA prepro (secretory signal peptide)"
     CDS             625..1905
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             1933..2553
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2589..3175
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3176..3892
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    4018..4139
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4146..4601)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4628..4732
                     /label="AmpR promoter"
     promoter        4734..5091
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5126..5917
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6152..6199
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6528..7116
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgagcggca cacgtgggtt cccgcacgtc cgcacacgtg
       61 ggttcccgca cgtccgcaca cgtgggttcc cgcacgtccg cacacgtggg ttcccgcacg
      121 tccgcacacg tgggttcccg cacgtccgca cacgtgggtt cccgcacgtc cgccggaatt
      181 gatcttcgtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagagc tacgcgtcga cgtcggccat agcggccgcg
      421 gaattgaaaa caaacagaca atctggtctg tttgtattat aagtaaggac tagtccatcg
      481 atcgatcccg ccgccaccat ggcgatggat gcaatgaaga gagggctctg ctgtgtgctg
      541 ctgctgtgtg gagcagtctt cgtttcgccc agccaggaaa tccatgcccg attcagaaga
      601 ggagccagat tggcgcgcca aatggtgtcg aataacgctt tagaaacaat tattaacgcc
      661 cggttaccag gcgaagaggg gctgtggcag attcatctgc aggacggaaa aatcagcgcc
      721 attgatgcgc aatccggcgt gatgcccata actgaaaaca gcctggatgc cgaacaaggt
      781 ttagttatac cgccgtttgt ggagccacat attcacctgg acaccacgca aaccgccgga
      841 caaccgaact ggaatcagtc cggcacgctg tttgaaggca ttgaacgctg ggccgagcgc
      901 aaagcgttat taacccatga cgatgtgaaa caacgcgcat ggcaaacgct gaaatggcag
      961 attgccaacg gcattcagca tgtgcgtacc catgtcgatg tttcggatgc aacgctaact
     1021 gcgctgaaag caatgctgga agtgaagcag gaagtcgcgc cgtggattga tctgcaaatc
     1081 gtcgccttcc ctcaggaagg gattttgtcg tatcccaacg gtgaagcgtt gctggaagag
     1141 gcgttacgct taggggcaga tgtagtgggg gcgattccgc attttgaatt tacccgtgaa
     1201 tacggcgtgg agtcgctgca taaaaccttc gccctggcgc aaaaatacga ccgtctcatc
     1261 gacgttcact gtgatgagat cgatgacgag cagtcgcgct ttgtcgaaac cgttgctgcc
     1321 ctggcgcacc atgaaggcat gggcgcgcga gtcaccgcca gccacaccac ggcaatgcac
     1381 tcctataacg gggcgtatac ctcacgcctg ttccgcttgc tgaaaatgtc cggtattaac
     1441 tttgtcgcca acccgctggt caatattcat ctgcaaggac gtttcgatac gtatccaaaa
     1501 cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg gcattaacgt ctgctttggt
     1561 cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg cgaatatgct gcaagtgctg
     1621 catatggggc tgcatgtttg ccagttgatg ggctacgggc agattaacga tggcctgaat
     1681 ttaatcaccc accacagcgc aaggacgttg aatttgcagg attacggcat tgccgccgga
     1741 aacagcgcca acctgattat cctgccggct gaaaatgggt ttgatgcgct gcgccgtcag
     1801 gttccggtac gttattcggt acgtggcggc aaggtgattg ccagcacaca accggcacaa
     1861 accaccgtat atctggagca gccagaagcc atcgattaca aacgtatcag cggagctaat
     1921 ggcgtcatgg ctaagatcgt ggaagtcaaa cacccactcg tcaaacacaa gctgggactg
     1981 atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga agtgggtagc
     2041 ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtaactat cgaaggctgg
     2101 aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt gccaattctg
     2161 cgtgcgggtc ttggtatgat ggacggtgtg ctggaaaacg ttccgagcgc gcgcatcagc
     2221 gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt ccagaaactg
     2281 gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc aaccggtggt
     2341 tccgttatcg cgaccatcga cctgctgaaa aaagcgggct gcagcagcat caaagttctg
     2401 gtgctggtag ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc ggacgtcgaa
     2461 ctgtataccg catcgattga tcagggactg aacgagcacg gatacattat tccgggcctc
     2521 ggcgatgccg gtgacaaaat ctttggtacg aaataagctt gtcgaaattc gctagccgcg
     2581 cggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2641 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2701 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2761 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2821 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2881 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2941 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3001 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3061 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3121 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     3181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3361 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3601 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3661 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3781 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3841 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     3901 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3961 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4021 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4081 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     4141 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4201 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4261 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4321 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4381 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4441 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4501 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4561 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4681 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     4741 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4801 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4861 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4921 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5041 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     5101 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5161 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5221 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5281 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     5341 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5401 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5461 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5521 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5581 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5641 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5701 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5761 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5821 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5881 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5941 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6001 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6061 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     6121 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     6181 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     6241 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     6301 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     6361 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6661 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7201 tctgtggata accgtattac cgccatgcat
//
