LOCUS       V016185                 7074 bp    DNA     circular SYN 29-JUN-2020
DEFINITION  Exported.
ACCESSION   V016185
VERSION     V016185
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2020) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (16-FEB-2020) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
FEATURES             Location/Qualifiers
     source          1..7074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..235
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    273..300
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      331..348
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     349..453
                     /note="tPA prepro (secretory signal peptide)"
     CDS             469..1749
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             1777..2397
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2433..3019
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3020..3736
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3862..3983
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3990..4445)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4472..4576
                     /label="AmpR promoter"
     promoter        4578..4935
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4970..5761
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5996..6043
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6372..6960
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgagatctt cgtgatgcgg ttttggcagt acatcaatgg
       61 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      121 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      181 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctacgcg
      241 tcgacgtcgg ccatagcggc cgcggaattg aaaacaaaca gacaatctgg tctgtttgta
      301 ttataagtaa ggactagtcc atcgatcgat cccgccgcca ccatggcgat ggatgcaatg
      361 aagagagggc tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc gcccagccag
      421 gaaatccatg cccgattcag aagaggagcc agattggcgc gccaaatggt gtcgaataac
      481 gctttagaaa caattattaa cgcccggtta ccaggcgaag aggggctgtg gcagattcat
      541 ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg gcgtgatgcc cataactgaa
      601 aacagcctgg atgccgaaca aggtttagtt ataccgccgt ttgtggagcc acatattcac
      661 ctggacacca cgcaaaccgc cggacaaccg aactggaatc agtccggcac gctgtttgaa
      721 ggcattgaac gctgggccga gcgcaaagcg ttattaaccc atgacgatgt gaaacaacgc
      781 gcatggcaaa cgctgaaatg gcagattgcc aacggcattc agcatgtgcg tacccatgtc
      841 gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc tggaagtgaa gcaggaagtc
      901 gcgccgtgga ttgatctgca aatcgtcgcc ttccctcagg aagggatttt gtcgtatccc
      961 aacggtgaag cgttgctgga agaggcgtta cgcttagggg cagatgtagt gggggcgatt
     1021 ccgcattttg aatttacccg tgaatacggc gtggagtcgc tgcataaaac cttcgccctg
     1081 gcgcaaaaat acgaccgtct catcgacgtt cactgtgatg agatcgatga cgagcagtcg
     1141 cgctttgtcg aaaccgttgc tgccctggcg caccatgaag gcatgggcgc gcgagtcacc
     1201 gccagccaca ccacggcaat gcactcctat aacggggcgt atacctcacg cctgttccgc
     1261 ttgctgaaaa tgtccggtat taactttgtc gccaacccgc tggtcaatat tcatctgcaa
     1321 ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc gcgttaaaga gatgctggag
     1381 tccggcatta acgtctgctt tggtcacgat gatgtcttcg atccgtggta tccgctggga
     1441 acggcgaata tgctgcaagt gctgcatatg gggctgcatg tttgccagtt gatgggctac
     1501 gggcagatta acgatggcct gaatttaatc acccaccaca gcgcaaggac gttgaatttg
     1561 caggattacg gcattgccgc cggaaacagc gccaacctga ttatcctgcc ggctgaaaat
     1621 gggtttgatg cgctgcgccg tcaggttccg gtacgttatt cggtacgtgg cggcaaggtg
     1681 attgccagca cacaaccggc acaaaccacc gtatatctgg agcagccaga agccatcgat
     1741 tacaaacgta tcagcggagc taatggcgtc atggctaaga tcgtggaagt caaacaccca
     1801 ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa gcgctttcgc
     1861 gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga cctcgaaacg
     1921 gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat caaaggtaag
     1981 aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg tgtgctggaa
     2041 aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag
     2101 ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc gctgatcgtt
     2161 gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg
     2221 ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat cgctgcgctg
     2281 gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgatcaggg actgaacgag
     2341 cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg tacgaaataa
     2401 gcttgtcgaa attcgctagc cgcgcggatc cgcccctctc cctccccccc ccctaacgtt
     2461 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     2521 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     2581 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     2641 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     2701 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     2761 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     2821 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     2881 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     2941 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     3001 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3061 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3121 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3181 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3241 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3301 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3361 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3421 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3481 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3541 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3601 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3661 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3721 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt
     3781 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     3841 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     3901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3961 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt
     4021 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     4081 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     4141 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     4201 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     4261 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     4321 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     4381 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     4441 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4501 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4561 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     4621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4681 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4741 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4921 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     5161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5461 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     5521 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5581 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5641 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5701 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5761 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5821 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5881 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     5941 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     6001 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     6061 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     6121 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     6181 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
     6241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6481 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
