LOCUS       V015297                 6854 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V015297
VERSION     V015297
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2020) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (16-FEB-2020) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
            On May 13, 2021 this sequence version replaced MT086573.1.
FEATURES             Location/Qualifiers
     source          1..6854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..80
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      111..128
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     129..233
                     /note="tPA prepro (secretory signal peptide)"
     CDS             249..1529
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             1557..2177
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2213..2799
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2800..3516
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3642..3763
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3770..4225)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4252..4356
                     /label="AmpR promoter"
     promoter        4358..4715
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4750..5541
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5776..5823
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6152..6740
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgacgtcgg ccatagcggc cgcggaattg aaaacaaaca
       61 gacaatctgg tctgtttgta ttataagtaa ggactagtcc atcgatcgat cccgccgcca
      121 ccatggcgat ggatgcaatg aagagagggc tctgctgtgt gctgctgctg tgtggagcag
      181 tcttcgtttc gcccagccag gaaatccatg cccgattcag aagaggagcc agattggcgc
      241 gccaaatggt gtcgaataac gctttagaaa caattattaa cgcccggtta ccaggcgaag
      301 aggggctgtg gcagattcat ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg
      361 gcgtgatgcc cataactgaa aacagcctgg atgccgaaca aggtttagtt ataccgccgt
      421 ttgtggagcc acatattcac ctggacacca cgcaaaccgc cggacaaccg aactggaatc
      481 agtccggcac gctgtttgaa ggcattgaac gctgggccga gcgcaaagcg ttattaaccc
      541 atgacgatgt gaaacaacgc gcatggcaaa cgctgaaatg gcagattgcc aacggcattc
      601 agcatgtgcg tacccatgtc gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc
      661 tggaagtgaa gcaggaagtc gcgccgtgga ttgatctgca aatcgtcgcc ttccctcagg
      721 aagggatttt gtcgtatccc aacggtgaag cgttgctgga agaggcgtta cgcttagggg
      781 cagatgtagt gggggcgatt ccgcattttg aatttacccg tgaatacggc gtggagtcgc
      841 tgcataaaac cttcgccctg gcgcaaaaat acgaccgtct catcgacgtt cactgtgatg
      901 agatcgatga cgagcagtcg cgctttgtcg aaaccgttgc tgccctggcg caccatgaag
      961 gcatgggcgc gcgagtcacc gccagccaca ccacggcaat gcactcctat aacggggcgt
     1021 atacctcacg cctgttccgc ttgctgaaaa tgtccggtat taactttgtc gccaacccgc
     1081 tggtcaatat tcatctgcaa ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc
     1141 gcgttaaaga gatgctggag tccggcatta acgtctgctt tggtcacgat gatgtcttcg
     1201 atccgtggta tccgctggga acggcgaata tgctgcaagt gctgcatatg gggctgcatg
     1261 tttgccagtt gatgggctac gggcagatta acgatggcct gaatttaatc acccaccaca
     1321 gcgcaaggac gttgaatttg caggattacg gcattgccgc cggaaacagc gccaacctga
     1381 ttatcctgcc ggctgaaaat gggtttgatg cgctgcgccg tcaggttccg gtacgttatt
     1441 cggtacgtgg cggcaaggtg attgccagca cacaaccggc acaaaccacc gtatatctgg
     1501 agcagccaga agccatcgat tacaaacgta tcagcggagc taatggcgtc atggctaaga
     1561 tcgtggaagt caaacaccca ctcgtcaaac acaagctggg actgatgcgt gagcaagata
     1621 tcagcaccaa gcgctttcgc gaactcgctt ccgaagtggg tagcctgctg acttacgaag
     1681 cgaccgccga cctcgaaacg gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa
     1741 tcgaccagat caaaggtaag aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta
     1801 tgatggacgg tgtgctggaa aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc
     1861 gtaatgaaga aacgctggag ccggtaccgt acttccagaa actggtttct aacatcgatg
     1921 agcgtatggc gctgatcgtt gacccaatgc tggcaaccgg tggttccgtt atcgcgacca
     1981 tcgacctgct gaaaaaagcg ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc
     2041 cagaaggtat cgctgcgctg gaaaaagcgc acccggacgt cgaactgtat accgcatcga
     2101 ttgatcaggg actgaacgag cacggataca ttattccggg cctcggcgat gccggtgaca
     2161 aaatctttgg tacgaaataa gcttgtcgaa attcgctagc cgcgcggatc cgcccctctc
     2221 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     2281 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     2341 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     2401 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     2461 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     2521 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     2581 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     2641 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     2701 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     2761 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaggag
     2821 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2881 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2941 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3001 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3061 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3121 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3181 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3241 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3301 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3361 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3421 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3481 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     3541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     3601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     3661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
     3781 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3841 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3901 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3961 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4021 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4081 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4141 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4201 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4261 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4321 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     4381 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4441 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4501 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4561 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4621 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4681 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     4741 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4801 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4861 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4921 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4981 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5041 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5101 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5161 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5221 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     5281 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5341 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5401 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5461 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5521 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5581 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5641 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5701 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     5761 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     5821 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     5881 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     5941 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6841 ttaccgccat gcat
//
