LOCUS       V016405                 7389 bp    DNA     circular SYN 28-JAN-2020
DEFINITION  Exported.
ACCESSION   V016405
VERSION     V016405
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2019) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (21-OCT-2019) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
FEATURES             Location/Qualifiers
     source          1..7389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        33..336
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        347..550
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    588..615
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      646..663
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     664..768
     CDS             784..2064
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             2092..2712
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2748..3334
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3335..4051
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    4177..4298
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4305..4760)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4787..4891
                     /label="AmpR promoter"
     promoter        4893..5250
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5285..6076
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6311..6358
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6687..7275
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgaaattcc ggcgttacat aacttacggt aaatggcccg
       61 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      121 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      181 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      241 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      301 cagtacatct acgtattagt catcgctatt accatggaag atcttcgtga tgcggttttg
      361 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      421 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      481 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      541 aagcagagct acgcgtcgac gtcggccata gcggccgcgg aattgaaaac aaacagacaa
      601 tctggtctgt ttgtattata agtaaggact agtccatcga tcgatcccgc cgccaccatg
      661 gcgatggatg caatgaagag agggctctgc tgtgtgctgc tgctgtgtgg agcagtcttc
      721 gtttcgccca gccaggaaat ccatgcccga ttcagaagag gagccagatt ggcgcgccaa
      781 atggtgtcga ataacgcttt agaaacaatt attaacgccc ggttaccagg cgaagagggg
      841 ctgtggcaga ttcatctgca ggacggaaaa atcagcgcca ttgatgcgca atccggcgtg
      901 atgcccataa ctgaaaacag cctggatgcc gaacaaggtt tagttatacc gccgtttgtg
      961 gagccacata ttcacctgga caccacgcaa accgccggac aaccgaactg gaatcagtcc
     1021 ggcacgctgt ttgaaggcat tgaacgctgg gccgagcgca aagcgttatt aacccatgac
     1081 gatgtgaaac aacgcgcatg gcaaacgctg aaatggcaga ttgccaacgg cattcagcat
     1141 gtgcgtaccc atgtcgatgt ttcggatgca acgctaactg cgctgaaagc aatgctggaa
     1201 gtgaagcagg aagtcgcgcc gtggattgat ctgcaaatcg tcgccttccc tcaggaaggg
     1261 attttgtcgt atcccaacgg tgaagcgttg ctggaagagg cgttacgctt aggggcagat
     1321 gtagtggggg cgattccgca ttttgaattt acccgtgaat acggcgtgga gtcgctgcat
     1381 aaaaccttcg ccctggcgca aaaatacgac cgtctcatcg acgttcactg tgatgagatc
     1441 gatgacgagc agtcgcgctt tgtcgaaacc gttgctgccc tggcgcacca tgaaggcatg
     1501 ggcgcgcgag tcaccgccag ccacaccacg gcaatgcact cctataacgg ggcgtatacc
     1561 tcacgcctgt tccgcttgct gaaaatgtcc ggtattaact ttgtcgccaa cccgctggtc
     1621 aatattcatc tgcaaggacg tttcgatacg tatccaaaac gtcgcggcat cacgcgcgtt
     1681 aaagagatgc tggagtccgg cattaacgtc tgctttggtc acgatgatgt cttcgatccg
     1741 tggtatccgc tgggaacggc gaatatgctg caagtgctgc atatggggct gcatgtttgc
     1801 cagttgatgg gctacgggca gattaacgat ggcctgaatt taatcaccca ccacagcgca
     1861 aggacgttga atttgcagga ttacggcatt gccgccggaa acagcgccaa cctgattatc
     1921 ctgccggctg aaaatgggtt tgatgcgctg cgccgtcagg ttccggtacg ttattcggta
     1981 cgtggcggca aggtgattgc cagcacacaa ccggcacaaa ccaccgtata tctggagcag
     2041 ccagaagcca tcgattacaa acgtatcagc ggagctaatg gcgtcatggc taagatcgtg
     2101 gaagtcaaac acccactcgt caaacacaag ctgggactga tgcgtgagca agatatcagc
     2161 accaagcgct ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc
     2221 gccgacctcg aaacggaaaa agtaactatc gaaggctgga acggcccggt agaaatcgac
     2281 cagatcaaag gtaagaaaat taccgttgtg ccaattctgc gtgcgggtct tggtatgatg
     2341 gacggtgtgc tggaaaacgt tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat
     2401 gaagaaacgc tggagccggt accgtacttc cagaaactgg tttctaacat cgatgagcgt
     2461 atggcgctga tcgttgaccc aatgctggca accggtggtt ccgttatcgc gaccatcgac
     2521 ctgctgaaaa aagcgggctg cagcagcatc aaagttctgg tgctggtagc tgcgccagaa
     2581 ggtatcgctg cgctggaaaa agcgcacccg gacgtcgaac tgtataccgc atcgattgat
     2641 cagggactga acgagcacgg atacattatt ccgggcctcg gcgatgccgg tgacaaaatc
     2701 tttggtacga aataagcttg tcgaaattcg ctagccgcgc ggatccgccc ctctccctcc
     2761 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2821 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2881 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2941 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3001 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3061 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3121 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3181 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3241 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3301 ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt
     3361 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3421 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3481 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3541 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3601 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3661 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3721 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3781 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3841 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3901 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     3961 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4021 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcataatc
     4081 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4141 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4201 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4261 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     4321 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4381 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4441 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4501 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4561 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4621 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4681 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4741 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4801 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4861 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     4921 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4981 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5041 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5101 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5161 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5221 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     5281 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5341 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5401 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5461 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5521 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5581 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5641 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5701 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5761 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     5821 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5881 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5941 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6001 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6061 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6121 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6181 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6241 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     6301 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     6361 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     6421 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     6481 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     6541 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6601 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7321 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7381 gccatgcat
//
