LOCUS       V016540                 7541 bp    DNA     circular SYN 29-JAN-2020
DEFINITION  Exported.
ACCESSION   V016540
VERSION     V016540
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Taghavi M, Parham A, Dehghani H, Naderi-Meshkin H.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2019) Department of Basic Sciences, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 91779-48974, Iran
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (04-OCT-2019) Department of Basic Sciences, Faculty of Veterinary
            Medicine, Ferdowsi University of Mashhad, Azadi Square, Mashhad,
            Razavi Khorasan 91779-48974, Iran"
FEATURES             Location/Qualifiers
     source          1..7541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   29..172
                     /rpt_unit_range=29 .. 52
                     /rpt_unit_seq="cacacgtgggttcccgcacgtccg"
     regulatory      29..172
                     /label="sextuplicate hypoxia-response element"
                     /note="sextuplicate hypoxia-response element"
                     /regulatory_class="response_element"
     enhancer        185..488
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        499..702
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    740..767
                     /label="CuO"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     regulatory      798..815
                     /note="Kozak consensus sequence; vertebrate consensus
                     sequence for strong initiation of translation"
                     /regulatory_class="ribosome_binding_site"
     sig_peptide     816..920
     CDS             936..2216
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     CDS             2244..2864
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Escherichia
                     coli O139:H28 (strain E24377A / ETEC). Accession#: A7ZPU1"
     misc_feature    2900..3486
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3487..4203
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    4329..4450
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4457..4912)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4939..5043
                     /label="AmpR promoter"
     promoter        5045..5402
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5437..6228
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6463..6510
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6839..7427
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaaccgc tcgagcggca cacgtgggtt cccgcacgtc cgcacacgtg
       61 ggttcccgca cgtccgcaca cgtgggttcc cgcacgtccg cacacgtggg ttcccgcacg
      121 tccgcacacg tgggttcccg cacgtccgca cacgtgggtt cccgcacgtc cgccggaatt
      181 ccggcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      241 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      301 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      361 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      421 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      481 ttaccatgga agatcttcgt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      541 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      601 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      661 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctacgcgtcg acgtcggcca
      721 tagcggccgc ggaattgaaa acaaacagac aatctggtct gtttgtatta taagtaagga
      781 ctagtccatc gatcgatccc gccgccacca tggcgatgga tgcaatgaag agagggctct
      841 gctgtgtgct gctgctgtgt ggagcagtct tcgtttcgcc cagccaggaa atccatgccc
      901 gattcagaag aggagccaga ttggcgcgcc aaatggtgtc gaataacgct ttagaaacaa
      961 ttattaacgc ccggttacca ggcgaagagg ggctgtggca gattcatctg caggacggaa
     1021 aaatcagcgc cattgatgcg caatccggcg tgatgcccat aactgaaaac agcctggatg
     1081 ccgaacaagg tttagttata ccgccgtttg tggagccaca tattcacctg gacaccacgc
     1141 aaaccgccgg acaaccgaac tggaatcagt ccggcacgct gtttgaaggc attgaacgct
     1201 gggccgagcg caaagcgtta ttaacccatg acgatgtgaa acaacgcgca tggcaaacgc
     1261 tgaaatggca gattgccaac ggcattcagc atgtgcgtac ccatgtcgat gtttcggatg
     1321 caacgctaac tgcgctgaaa gcaatgctgg aagtgaagca ggaagtcgcg ccgtggattg
     1381 atctgcaaat cgtcgccttc cctcaggaag ggattttgtc gtatcccaac ggtgaagcgt
     1441 tgctggaaga ggcgttacgc ttaggggcag atgtagtggg ggcgattccg cattttgaat
     1501 ttacccgtga atacggcgtg gagtcgctgc ataaaacctt cgccctggcg caaaaatacg
     1561 accgtctcat cgacgttcac tgtgatgaga tcgatgacga gcagtcgcgc tttgtcgaaa
     1621 ccgttgctgc cctggcgcac catgaaggca tgggcgcgcg agtcaccgcc agccacacca
     1681 cggcaatgca ctcctataac ggggcgtata cctcacgcct gttccgcttg ctgaaaatgt
     1741 ccggtattaa ctttgtcgcc aacccgctgg tcaatattca tctgcaagga cgtttcgata
     1801 cgtatccaaa acgtcgcggc atcacgcgcg ttaaagagat gctggagtcc ggcattaacg
     1861 tctgctttgg tcacgatgat gtcttcgatc cgtggtatcc gctgggaacg gcgaatatgc
     1921 tgcaagtgct gcatatgggg ctgcatgttt gccagttgat gggctacggg cagattaacg
     1981 atggcctgaa tttaatcacc caccacagcg caaggacgtt gaatttgcag gattacggca
     2041 ttgccgccgg aaacagcgcc aacctgatta tcctgccggc tgaaaatggg tttgatgcgc
     2101 tgcgccgtca ggttccggta cgttattcgg tacgtggcgg caaggtgatt gccagcacac
     2161 aaccggcaca aaccaccgta tatctggagc agccagaagc catcgattac aaacgtatca
     2221 gcggagctaa tggcgtcatg gctaagatcg tggaagtcaa acacccactc gtcaaacaca
     2281 agctgggact gatgcgtgag caagatatca gcaccaagcg ctttcgcgaa ctcgcttccg
     2341 aagtgggtag cctgctgact tacgaagcga ccgccgacct cgaaacggaa aaagtaacta
     2401 tcgaaggctg gaacggcccg gtagaaatcg accagatcaa aggtaagaaa attaccgttg
     2461 tgccaattct gcgtgcgggt cttggtatga tggacggtgt gctggaaaac gttccgagcg
     2521 cgcgcatcag cgttgtcggt atgtaccgta atgaagaaac gctggagccg gtaccgtact
     2581 tccagaaact ggtttctaac atcgatgagc gtatggcgct gatcgttgac ccaatgctgg
     2641 caaccggtgg ttccgttatc gcgaccatcg acctgctgaa aaaagcgggc tgcagcagca
     2701 tcaaagttct ggtgctggta gctgcgccag aaggtatcgc tgcgctggaa aaagcgcacc
     2761 cggacgtcga actgtatacc gcatcgattg atcagggact gaacgagcac ggatacatta
     2821 ttccgggcct cggcgatgcc ggtgacaaaa tctttggtac gaaataagct tgtcgaaatt
     2881 cgctagccgc gcggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     2941 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     3001 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     3061 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     3121 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     3181 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     3241 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     3301 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     3361 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     3421 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     3481 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3541 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3601 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3661 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3721 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3781 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3841 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3901 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3961 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4021 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4081 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4141 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4201 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     4261 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     4321 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4381 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4441 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4501 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4561 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4621 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4681 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4741 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4801 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4861 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     4921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5041 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5101 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5221 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5281 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5341 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5401 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5461 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5521 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5581 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     5641 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5701 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5761 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5821 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5881 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5941 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     6001 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6061 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6121 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6181 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6241 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6301 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6361 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     6421 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     6481 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     6541 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     6601 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     6661 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     6721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6961 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     7021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7501 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
