LOCUS       40924_32425       10933 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMSW107, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Wecker MS, Beaton S, Chado R, Ghirardi ML.
  TITLE     Development of a Rhodobacter capsulatus self-reporting model system 
            for optimizing light-dependent, [FeFe]-hydrogenase-driven H 
            production
  JOURNAL   Biotechnol. Bioeng. (2016) In press
  PUBMED    27531314
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Wecker MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 
            Cherryvale Road, Boulder, CO 80303, USA
REFERENCE   3  (bases 1 to 10933)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2016) Molecular Biology, GeneBiologics, 1047 Cherryvale 
            Road, Boulder, CO 80303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1693..1723
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1832..2548)
                     /label=EmGFP
                     /note="Emerald GFP"
     misc_feature    complement(2549..2770)
                     /gene="HupSL-emGFP"
                     /note="HupSL remnant; acts as signal sequence for the
                     downstream emGFP"
     regulatory      complement(2771..3299)
                     /regulatory_class="promoter"
     primer_bind     3306..3322
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3356..3372)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3405..3423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3433..3449)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3881..4491
                     /note="fru promoter; derived from Rhodobacter capsulatus"
                     /regulatory_class="promoter"
     CDS             4492..5544
                     /codon_start=1
                     /gene="hydE"
                     /product="HydE"
                     /label=hydE
                     /note="derived from Clostridium acetobutylicum strain DSM
                     1731; codon-optimized for Rhodobacter capsulatus"
                     /protein_id="AOF40442.1"
                     /translation="MDNIIKLINKAEVTHDLTKDELVTLLKDDTHNEEIYKAADRVREK
                     YVGEEVHLRGLIEFSNICKRNCMYCGLRRDNKNIKRYRLEPDEIIHLAKSAKNYGYQTV
                     VLQSGEDDYYTVEKMKYIVSEIKKLNMAITLSIGEKTFEEYEEYRKSGADRYLIRIETT
                     DKELYEKLDPKMSHENRINCLKNLRKLGYEVGSGCLVGLPNQTIESLADDILFFKEIDA
                     DMIGVGPFIPNEDTPLGEEKGGEFFMSVKVTALIRLLLPDINIPATTAMESLYPNGRSI
                     ALTSGANVVMPNVTEGEYRKLYALYPGKICVNDTPGHCRQCISLKINKINRKVSATKGF
                     RKKSYKESIG"
     gene            4492..5544
                     /gene="hydE"
                     /label=hydE
     CDS             5558..6793
                     /codon_start=1
                     /gene="hydF"
                     /product="HydF"
                     /label=hydF
                     /note="derived from Clostridium acetobutylicum strain DSM
                     1731; codon-optimized for Rhodobacter capsulatus"
                     /protein_id="AOF40443.1"
                     /translation="MNELNSTPKGERLHIALFGKTNVGKSSVINALTSQEIALVSNVKG
                     TTTDPVYKAMELLPLGPVMLIDTAGLDDISDLGELRRGKTLEVLSKTDVAILVFDVESG
                     ITEYDKNIYSLLLEKKIPLIGVLNKIDKKDYKLEDYTSQFKIPIVPISALNNKGINNLK
                     DELIRLAPENDDKFKIVGDLLSPGDIAVLVTPIDKAAPKGRLILPQQQTIRDILESDAI
                     AMVTKEFELRETLDSLRKKPKIVITDSQVFLKVAADTPKDILMTSFSILMARHKGDLIE
                     LARGARAIEDLKDGDKILIAEACTHHRQSDDIGKVKIPRWLRQKTGKKLEFDFSSGFSF
                     PPNIEDYALIVHCAGCMLNRRSMLHRIESSVKKQIPIVNYGVLIAYVQGILPRALKPFP
                     YADRIFNQSSRN"
     gene            5558..6793
                     /gene="hydF"
                     /label=hydF
     CDS             6807..8225
                     /codon_start=1
                     /gene="hydG"
                     /product="HydG"
                     /label=hydG
                     /note="derived from Clostridium acetobutylicum strain DSM
                     1731; codon-optimized for Rhodobacter capsulatus"
                     /protein_id="AOF40444.1"
                     /translation="MYNVKSKVATEFISDEEIIDSLEYAKQNKSNRELIDSIIEKAKEC
                     KGLTHRDAAVLLECDLEDENEKMFKLAREIKQKFYGNRIVMFAPLYLSNYCVNGCVYCP
                     YHHKNKHIARKKLSQEDVKRETIALQDMGHKRLALEAGEDPVNNPIEYILDCIKTIYSI
                     KHKNGAIRRVNVNIAATTVENYKKLKDAGIGTYILFQETYNKKSYEELHPTGPKHDYAY
                     HTEAMDRAMEGGIDDVGIGVLFGLNMYKYDFVGLLMHAEHLEAAMGVGPHTISVPRIRP
                     ADDIDPENFSNAISDEIFEKIVAIIRIAVPYTGMIVSTRESKKTRERVLELGISQISGG
                     SSTSVGGYVESEPEEDNSSQFEVNDNRTLDEIVNWLLEMNYIPSFCTACYREGRTGDRF
                     MSLVKSGQIANCCQPNALMTLKEYLEDYASSNTQKNGEALIASEVEKIPNEKVKSIVKK
                     HLTELKEGQRDFRF"
     gene            6807..8225
                     /gene="hydG"
                     /label=hydG
     CDS             complement(8482..9141)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(9142..9911)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtacctt acttgtacag ctcgtccatg ccgagagtga
     1861 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     1921 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     1981 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     2041 ggcgggtctt gaagttcacc ttgatgccgt tcttctgctt gtcggcggtg atatagacct
     2101 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     2161 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     2221 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     2281 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
     2341 gcacgccgta ggtcaaggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     2401 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     2461 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     2521 tgaacagctc ctcgcccttg ctcaccatat gtgcaggtcg agtactcgac tctagccgtg
     2581 cggtgcattc aagcccatgc acccagacga ccggggtgcg cggcttcgtt tccatggctt
     2641 cggcgatttt cggcacgaag gagggcccaa gccccaaagc tgctgcggtg agcgaacaga
     2701 acttcatgaa gctgcgccgg gtgatcccct gacgccgcat cacgtcatag aaagtttcga
     2761 tgtccgacaa ttgtccctcc cttgccttgt gacgcgccgt gacctcctcg gcgcgcccat
     2821 gggtagagtg gaattctcat ggttgggccg gtttccgcca atgagaaacg gcaaattgtc
     2881 gcagaaaaat ttttccttgt ttttcaatgg tgtgtgattt tgtcacgtaa aaaaaccgaa
     2941 agtattttga ccgattccat ttccacttcg gtcagttgct ttccaatttt gccgccgcga
     3001 caagggcctg acccagggca aggcccccat cgttggccgg gatgcggccc tgcgtcagaa
     3061 tgccttgatc ggcaaggaaa tttcgcgtca tggttgcaag tccggaattc tgaaaacagc
     3121 cgcccgtcag cgccaccgcc tcggcctgtc cggcttcgat cagccgccgc gcttcggcgg
     3181 caaaagcctg cgcaaggctg gcgtgcagcg catgggcgac acggtcgggg cgctcgcctg
     3241 ccgccagaag ttgaaacagc ggcgccgggt cgatcgcccc gccgacgcag ggcagatccc
     3301 gagctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc
     3361 actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta
     3421 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     3481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     3541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     3601 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3661 taggccgact gcgatgagtg gcagggcggg gcgtaatttt tttaaggcag ttattggtgc
     3721 ccttaaacgc ctggtgctac gcctgaataa gtgataataa gcggatgaat ggcagaaatt
     3781 cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag ccgcttatgt
     3841 ctattgctgg tttaccggtc tcgagcgtgc ccgccacgcc ccgggcgcgg aaaaaggcgg
     3901 cgatgcgggc gtaatcctgc catgtccggg gcggttccag cgcccggccg gtctgcgccc
     3961 gataggcggc ctgcgcggcc cggtcctcga acagatcgcg gcgataggcg cccatgtgca
     4021 gatcgccgtc cagcgtcacc gccagccact ggccccgcca gcgcatctgc cggtcgcgat
     4081 aggcggggtg gatgccctgc accagcgggc tttgcacaag gtcgtccggc accggcatca
     4141 gatagggcgc gacatcgggc agccagtcgg cgggcaggat cagcacatcg gcgggggggc
     4201 tgccggtgac gaagcccatc atcaccgccc cgtaaagcgc gtcaaagggg cggggcgtca
     4261 catgcaccgc cacgccggtt tcgcgcgcga actctgcccc atgggcgatg gccgggcccg
     4321 caatcgggcc gccgtcgcgg gtcagcacct cgagcgcctc ggcgcgggtc aggctcagcg
     4381 ccaatatgac aattgttaca agccgggccg tcacgggcga catactccat tcatctgccg
     4441 ggcgcttcgt cgcctgcaag agcatcgtac gtccgatgcc gaggaggaac catggacaac
     4501 atcatcaagc tcatcaacaa ggccgaagtc acgcacgacc tgaccaagga tgagcttgtc
     4561 accctgctga aggatgacac ccacaacgaa gagatctata aagcggccga tcgcgtccgg
     4621 gagaaatacg tgggcgaaga ggtgcatctt cgcgggctta tcgaattctc gaacatctgc
     4681 aagcggaact gcatgtactg cggccttcgc cgcgacaaca agaacatcaa gcggtaccgg
     4741 ctcgagcccg atgagatcat ccatcttgcg aagtccgcca aaaactacgg ctatcagacg
     4801 gtggtgctgc aatcgggcga agatgactac tataccgtcg agaagatgaa gtatattgtc
     4861 agcgagatca agaagctcaa tatggcgatc accctgagca tcggcgaaaa gacgttcgaa
     4921 gaatatgaag aatatcggaa gtcgggcgcg gaccgctatc tgatccggat cgaaaccacc
     4981 gataaagagc tctacgagaa gctggacccg aagatgagcc atgaaaaccg gatcaactgc
     5041 ctgaagaacc tgcgcaagct tggctatgaa gtggggtccg ggtgcctggt ggggctgccg
     5101 aaccagacca tcgaatcgct ggccgatgat atcctcttct ttaaagagat cgatgcggat
     5161 atgatcgggg tgggcccctt catcccgaac gaggatacgc ccctcggcga agaaaaaggc
     5221 ggggagttct tcatgagcgt gaaggtgacg gcgcttatcc ggctgctgct gcccgacatc
     5281 aacatcccgg ccacgaccgc gatggagtcg ctctatccga acgggcgctc gatcgcgctg
     5341 acctcggggg ccaacgtcgt catgcccaac gtgaccgaag gcgaatatcg caagctgtat
     5401 gccctgtatc ccgggaagat ctgcgtcaac gataccccgg gccattgccg ccagtgcatc
     5461 agcctgaaga tcaacaaaat caatcggaaa gtctcggcga ccaagggctt ccgcaagaag
     5521 tcctataaag agagcatcgg ctgaggaggc gatagccatg aatgaactga acagcacccc
     5581 gaaaggcgag cggctccata ttgcattgtt cggcaaaacg aacgtgggca agagctccgt
     5641 catcaacgcg ctgacgagcc aggagatcgc gctggtcagc aatgtgaagg ggaccaccac
     5701 cgatccggtg tacaaagcga tggagctgct gccccttggc cccgtgatgc ttatcgatac
     5761 ggccggcctg gacgacatct cggacctggg cgaactccgc cggggcaaga cgctggaggt
     5821 cctgtccaag accgatgtgg cgatcctggt gttcgacgtc gagtccggca tcacggagta
     5881 cgataagaat atctacagcc ttctgctgga gaaaaagatc ccgctgatcg gcgtcctcaa
     5941 caagatcgac aagaaggact acaagctgga ggattatacc tcgcaattca agatcccgat
     6001 cgtgccgatc tcggcgctga acaacaaggg gatcaataac ctgaaggacg aactgatccg
     6061 gcttgcgccg gaaaatgacg acaaattcaa gatcgtgggc gacctgcttt cgcccgggga
     6121 tatcgccgtg ctcgtcacgc ccatcgataa agccgccccg aaaggccggc tgattctgcc
     6181 gcagcagcag acgatccgcg acatcctgga aagcgacgcg atcgcgatgg tgaccaaaga
     6241 gttcgaactt cgcgaaaccc tggactcgct gcggaaaaag cccaagatcg tgatcaccga
     6301 tagccaggtg ttcctgaagg tcgccgccga cacccccaaa gacatcctta tgacctcgtt
     6361 ctcgatcctg atggcgcgcc acaaagggga ccttatcgaa ctggcgcgcg gcgcccgggc
     6421 gatcgaagat cttaaggatg gcgacaagat cctgatcgcg gaagcgtgca cccaccatcg
     6481 gcaatcggac gatatcggca aggtgaaaat cccgcgctgg ctgcgccaga aaaccgggaa
     6541 gaagctcgaa tttgatttct ccagcgggtt cagcttcccg ccgaacatcg aagattatgc
     6601 gctgattgtc cattgtgcgg gctgcatgct gaatcgccgc agcatgctcc atcgcatcga
     6661 gtccagcgtc aagaagcaga ttccgatcgt gaactacggc gtgctgatcg cctatgtcca
     6721 gggcatcctg ccccgggccc ttaagccctt cccctatgcc gaccgcatct tcaaccagtc
     6781 gtcgcggaat tgaggaggcg atagccatgt acaacgtcaa gagcaaagtc gcgacggaat
     6841 tcatctcgga cgaagagatc atcgattcgc tggagtatgc gaagcagaac aagtcgaacc
     6901 gggagctgat cgactccatt atcgaaaagg cgaaagaatg caagggcctg acgcatcggg
     6961 atgccgccgt gctgcttgag tgcgacctgg aagatgaaaa cgagaagatg ttcaagcttg
     7021 cgcgcgaaat caagcagaag ttctacggca accggatcgt catgtttgcg ccgctgtatc
     7081 tttcgaacta ttgcgtgaac ggctgcgtgt attgccccta ccatcataag aacaaacaca
     7141 ttgcgcggaa gaagctgtcg caagaggacg tgaaacgcga aacgattgcc ctccaggaca
     7201 tgggccacaa gcggctggcc ctggaagccg gcgaggatcc ggtgaacaac cccatcgagt
     7261 acatcctgga ttgcatcaag accatctata gcattaagca caagaacggc gcgatccgcc
     7321 gcgtcaatgt gaatatcgcg gccaccacgg tggagaacta caaaaagctg aaggatgcgg
     7381 gcatcggcac ctatatcctg ttccaggaga cgtacaacaa gaagagctat gaagaactgc
     7441 atcccacggg gccgaagcac gattatgcgt accatacgga agcgatggat cgggcgatgg
     7501 agggcgggat cgacgatgtc ggcatcgggg tcctgtttgg gctcaatatg tataagtacg
     7561 actttgtcgg gctgctgatg catgcggagc atctggaggc cgcgatgggg gtgggcccgc
     7621 acaccatcag cgtgccccgc atccggccgg ccgacgacat cgacccggag aacttctcga
     7681 acgccatctc ggatgaaatc ttcgagaaga tcgtggcgat cattcgcatc gcggtgccgt
     7741 acacgggcat gatcgtcagc acgcgcgagt cgaaaaagac gcgcgagcgc gtgctggagc
     7801 tgggcatctc gcaaatctcg ggcgggtcgt cgaccagcgt gggcggctac gtcgaaagcg
     7861 aaccggaaga ggacaattcg tcgcagttcg aggtgaatga caaccggacg ctcgacgaaa
     7921 tcgtcaactg gcttctggag atgaactata tcccgagctt ttgcaccgcg tgttatcggg
     7981 aaggccgcac cggggaccgc tttatgtccc tcgtgaagtc gggccagatc gccaattgct
     8041 gccagccgaa cgccctgatg accctgaaag agtacctgga ggactacgcc agctcgaaca
     8101 cgcagaagaa tggcgaggcg ctgatcgcgt cggaggtgga gaagatcccg aatgagaaag
     8161 tgaagtcgat cgtgaaaaag cacctgacgg agctgaaaga agggcagcgc gatttccgct
     8221 tttgacctga ggccagtttg ctcaggctct ccccgtggag gtaataattg acgatatgat
     8281 catttattct gcctcccaga gcctgataaa aacggtgaat ccgttagcga ggtgccgccg
     8341 gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag
     8401 acaaggtata gggcggcgag gcggctacag ccgatagtct ggaacagcgc acttacgggt
     8461 tgctgcgcaa cccaagtgct accggcgcgg cagcgtgacc cgtgtcggcg gctccaacgg
     8521 ctcgccatcg tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg
     8581 ttcccattcc tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg
     8641 ctggctgggc ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt
     8701 cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac
     8761 caccacggcg gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac
     8821 gcggtcattg accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat
     8881 ccagcgctcg gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat
     8941 gagcttggaa agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac
     9001 gaggtgatgc agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc
     9061 atgcgctttg cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat
     9121 ttctctggac tgcgtggcca tgcttatctc catgcggtag ggtgccgcac ggttgcggca
     9181 ccatgcgcaa tcagctgcaa cttttcggca gcgcgacaac aattatgcgt tgcgtaaaag
     9241 tggcagtcaa ttacagattt tctttaacct acgcaatgag ctattgcggg gggtgccgca
     9301 atgagctgtt gcgtaccccc cttttttaag ttgttgattt ttaagtcttt cgcatttcgc
     9361 cctatatcta gttctttggt gcccaaagaa gggcacccct gcggggttcc cccacgcctt
     9421 cggcgcggct ccccctccgg caaaaagtgg cccctccggg gcttgttgat cgactgcgcg
     9481 gccttcggcc ttgcccaagg tggcgctgcc cccttggaac ccccgcactc gccgccgtga
     9541 ggctcggggg gcaggcgggc gggcttcgcc ttcgactgcc cccactcgca taggcttggg
     9601 tcgttccagg cgcgtcaagg ccaagccgct gcgcggtcgc tgcgcgagcc ttgacccgcc
     9661 ttccacttgg tgtccaaccg gcaagcgaag cgcgcaggcc gcaggccgga ggcttttccc
     9721 cagagaaaat taaaaaaatt gatggggcaa ggccgcaggc cgcgcagttg gagccggtgg
     9781 gtatgtggtc gaaggctggg tagccggtgg gcaatccctg tggtcaagct cgtgggcagg
     9841 cgcagcctgt ccatcagctt gtccagcagg gttgtccacg ggccgagcga agcgagccag
     9901 ccggtggccg ctcgcggcca tcgtccacat atccacgggc tggcaaggga gcgcagcgac
     9961 cgcgcagggc gaagcccgga gagcaagccc gtagggcgcc gcagccgccg taggcggtca
    10021 cgactttgcg aagcaaagtc tagtgagtat actcaagcat tgagtggccc gccggaggca
    10081 ccgccttgcg ctgcccccgt cgagccggtt ggacaccaaa agggaggggc aggcatggcg
    10141 gcatacgcga tcatgcgatg caagaagctg gcgaaaatgg gcaacgtggc ggccagtctc
    10201 aagcacgcct accgcgagcg cgagacgccc aacgctgacg ccagcaggac gccagagaac
    10261 gagcactggg cggccagcag caccgatgaa gcgatgggcc gactgcgcga gttgctgcca
    10321 gagaagcggc gcaaggacgc tgtgttggcg gtcgagtacg tcatgacggc cagcccggaa
    10381 tggtggaagt cggccagcca agaacagcag gcggcgttct tcgagaaggc gcacaagtgg
    10441 ctggcggaca agtacggggc ggatcgcatc gtgacggcca gcatccaccg tgacgaaacc
    10501 agcccgcaca tgaccgcgtt cgtggtgccg ctgacgcagg acggcaggct gtcggccaag
    10561 gagttcatcg gcaacaaagc gcagatgacc cgcgaccaga ccacgtttgc ggccgctgtg
    10621 gccgatctag ggctgcaacg gggcatcgag ggcagcaagg cacgtcacac gcgcattcag
    10681 gcgttctacg aggccctgga gcggccacca gtgggccacg tcaccatcag cccgcaagcg
    10741 gtcgagccac gcgcctatgc accgcaggga ttggccgaaa agctgggaat ctcaaagcgc
    10801 gttgagacgc cggaagccgt ggccgaccgg ctgacaaaag cggttcggca ggggtatgag
    10861 cctgccctac aggccgccgc aggagcgcgt gagatgcgca agaaggccga tcaagcccaa
    10921 gagacggccc gag
//
