LOCUS       62056_17360        5560 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..518
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     CDS             1021..1428
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="VTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTFNVG
                     WPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKPPPP
                     LPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1438..1808
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     LTR             1945..2459
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(2631..2647)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2655..2685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2700..2721)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3009..3597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3771..4628)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4629..4733)
                     /label=AmpR promoter
ORIGIN
        1 naatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
       61 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
      121 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
      181 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
      241 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
      301 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
      361 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca
      421 ataaagcctc ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc
      481 tcagattgat tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg
      541 agacccctgc ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt
      601 tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc
      661 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg
      721 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc
      781 cgacctgagt cctaaaatcc cgatcgttta ggactctttg gtgcaccccc cttagaggag
      841 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
      901 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg
      961 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa atatgggccc gggctagcct
     1021 gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac
     1081 aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca
     1141 acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt
     1201 aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg
     1261 acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag
     1321 cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc
     1381 ccgcctcgat cctcccttta tccagccctc actccttctc taggcgcccc catatggcca
     1441 tatgagatct tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca
     1501 agagttacta acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac
     1561 gaagtctgga gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct
     1621 cacccttacc gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa
     1681 cctcgctgga aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac
     1741 ggcatcgcag cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca
     1801 tcctctagcc ctcgagaagc ttgccaccat ggtagcgaat tctgcagata tccagcacag
     1861 tggcggccgc tagcgtcgac ggtaccgcgg gcccgggatc cgataaaata aaagatttta
     1921 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     1981 taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat
     2041 caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag
     2101 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca
     2161 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     2221 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     2281 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt
     2341 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     2401 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg
     2461 ggtaacagtt tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta
     2521 atcctgactc aattagccac tgttttgaat ccacatactc caatactcct gaaatccatc
     2581 gatggagttc attatggaca gcgcagaaag agctggggag aattgtgaaa ttgttatccg
     2641 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2701 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2821 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4741 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4801 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     4861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     4921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     4981 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5041 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     5101 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     5161 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcg
     5221 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     5281 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     5341 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     5401 cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata tcagtggtcc
     5461 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     5521 taaaataaaa gattttattt agtctccaga aaaagggggg
//
