LOCUS       40924_32385        5602 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMSD1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Meng J, Wang H, Liu X, Lin J, Pang X, Lin J.
  TITLE     Construction of small plasmid vectors for use in genetic improvement
            of the extremely acidophilic Acidithiobacillus caldus
  JOURNAL   Microbiol. Res. 168 (8), 469-476 (2013)
  PUBMED    23639949
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Meng J, Wang H, Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2013) State Key Laboratory of Microbial 
            Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong 
            250100, China
REFERENCE   3  (bases 1 to 5602)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5602)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. 
            Res."; date: "2013"; volume: "168"; issue: "8"; pages: "469-476"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2013) State Key Laboratory of Microbial Technology, Shandong
            University, Shanda Nanlu 27#, Jinan, Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3215..3322
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             3425..4228
                     /codon_start=1
                     /gene="strA"
                     /product="Sm resistance protein A"
                     /label=strA
                     /protein_id="AGT62532.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3425..4228
                     /gene="strA"
                     /label=strA
     CDS             4228..5064
                     /codon_start=1
                     /gene="strB"
                     /product="Sm resistance protein B"
                     /label=strB
                     /protein_id="AGT62533.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            4228..5064
                     /gene="strB"
                     /label=strB
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccggcggc acctcgctaa cggattcacc gtttttatca ggctctggga
     2641 ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg
     2701 gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
     2761 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa
     2821 tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag
     2881 gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc
     2941 agtcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3001 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3061 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3121 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3181 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     3241 tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga
     3301 cctcgagggg gggcccggta cctacgacct caagaaatgc attcatgttt gacagcttat
     3361 catccataag gtttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
     3421 gtgtatgaat cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag
     3481 aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat
     3541 cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa
     3601 aggtcgaggt gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg
     3661 cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct
     3721 caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca
     3781 atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg
     3841 caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc tgcacgatct
     3901 tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt
     3961 tgtttgccat ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg
     4021 cacgggtctg atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact
     4081 catgattgct aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc
     4141 tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct
     4201 gcgattggac cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg
     4261 cacgtttcgc aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca
     4321 ttgccagacg ggactcctgc aatcgtcaag ggattgaaac ctatagaaga cattgctgat
     4381 gaactgcgcg gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc
     4441 ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc
     4501 gttgccgagc acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg
     4561 tatgccgcat ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca
     4621 gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac
     4681 gcggcgatta tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc
     4741 gatctgcatc atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc
     4801 ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga
     4861 gacgaccttt gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg
     4921 ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg cttatgggtg cctttccgca
     4981 gcttggaacg cggatggaga agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag
     5041 caggtgcgac agacgtcata ctagatatca agcgacttct cctatcccct gggaacacat
     5101 caatctcacc ggagaatatc gctggccaaa gccttagcgt aggattccgc cccttcccgc
     5161 aaacgacccc aaacaagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     5221 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     5281 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     5341 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     5401 attcatccca ggtggcactt ttcggggaaa tgtgcgcgcc cgcgttcctg ctggcgctgg
     5461 gcctgtttct ggcgctggac ttcccgctgt tccgtcagca gcttttcgcc cacggccttg
     5521 atgatcgcgg cggccttggc ctgcatatcc cgattcaacg gccccagggc gtccagaacg
     5581 ggcttcaggc gctcccgaag gt
//
