LOCUS       62056_17290        5839 bp DNA     circular SYN 30-JUN-2020
DEFINITION  Cloning vector pMS79, complete sequence.
ACCESSION   MT267319
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P, 
            Fussenegger M.
  TITLE     Phosphoregulated orthogonal signal transduction in mammalian cells
  JOURNAL   Nat Commun 11 (1), 3085 (2020)
  PUBMED    32555187
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Scheller L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment 
            AI), Station 19, Lausanne 1015, Switzerland
REFERENCE   3  (bases 1 to 5839)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station
            19, Lausanne 1015, Switzerland"
FEATURES             Location/Qualifiers
     source          1..5839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..230
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
     promoter        280..298
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    327..332
                     /label=Kozak+ATG
                     /note="Kozak+ATG"
     CDS             330..1391
                     /codon_start=1
                     /transl_table=11
                     /product="truncated DcuS"
                     /label=truncated DcuS
                     /protein_id="QKE44358.1"
                     /translation="MTSKVLKKILFGLEPYEISTLFEQRQAMLQSIKEGVVAVDDRGEV
                     TLINDAAQELLNYRKSQDDEKLSTLSHSWSQVVDVSEVLRDGTPRRDEEITIKDRLLLI
                     NTVPVRSNGVIIGAISTFRDKTEVRKLMQRLDGLVNYADALRERSHEFMNKLHVILGLL
                     HLKSYKQLEDYILKTANNYQEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSES
                     QLPDSGSEDQVATLITTLGNLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGI
                     APDKIDHIFDKGVSTKGSERGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGE
                     RSNRASASGSTGV"
     misc_feature    339..1361
                     /label=DucS cytoplasmatic domain
                     /note="DucS cytoplasmatic domain"
     polyA_signal    1428..1652
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1698..2126
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2140..2469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2536..3327
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3504..3637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3674..3690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3698..3714)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3722..3752)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3767..3788)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4076..4661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4835..5692)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5693..5797)
                     /label=AmpR promoter
ORIGIN
        1 agatctccac gcgtggtacc ctcgaggcga tctgcatctc aattagtcag caaccatagt
       61 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      121 ccatcgctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa gagctctctg
      241 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      301 gacccaagct gttcgaagcg gaattcacca tgactagtaa ggtactgaaa aaaatccttt
      361 tcggcctgga accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt
      421 ctatcaaaga aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg
      481 ccgcacaaga attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctaa
      541 gccactcatg gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt accccgcgcc
      601 gcgacgaaga gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca
      661 gtaatggcgt tatcatcggt gccatttcaa ccttcaggga caaaactgaa gtacgtaaac
      721 tgatgcagcg actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg
      781 aatttatgaa taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt
      841 tggaagatta cattctcaaa acagccaata actatcagga agagattggc tctctgctgg
      901 gtaagatcaa atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg
      961 atttaggcca tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg
     1021 accaggtcgc gacgctgatt accacgttgg gaaatctgat agaaaacgcg ctggaggcat
     1081 tagggccgga acccggaggc gaaattagcg taacattgca ctaccgtcac ggctggctgc
     1141 actgtgaagt taatgatgat ggaccgggga tcgcacccga taaaatcgat cacatttttg
     1201 acaaaggtgt ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac
     1261 aggtagaaaa tctcggcggc agcatcgccg tggaatcgga acccgggatt ttcacacaat
     1321 tttttgtcca gataccctgg gacggggaga ggtcgaacag agctagtgct agcggatcca
     1381 ccggtgtcta gaaagctttg aggccggcct gcagggatca gcctcgactg tgccttctag
     1441 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     1501 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     1561 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     1621 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     1681 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     1741 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     1801 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     1861 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     1921 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1981 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     2041 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     2101 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     2161 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     2341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     2401 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     2461 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     2521 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     2581 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     2641 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     2701 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     2761 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     2821 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     2881 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     2941 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3001 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3061 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3121 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3181 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3241 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3301 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3361 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3421 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3481 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3601 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3661 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3721 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3781 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3841 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3901 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3961 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4021 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4081 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4141 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4201 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4261 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4321 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4381 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4441 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4501 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4561 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     4621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5821 ctgacgtcga cggatcggg
//
