LOCUS       62056_17285        5428 bp DNA     circular SYN 30-JUN-2020
DEFINITION  Cloning vector pMS75, complete sequence.
ACCESSION   MT267318
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P, 
            Fussenegger M.
  TITLE     Phosphoregulated orthogonal signal transduction in mammalian cells
  JOURNAL   Nat Commun 11 (1), 3085 (2020)
  PUBMED    32555187
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Scheller L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment 
            AI), Station 19, Lausanne 1015, Switzerland
REFERENCE   3  (bases 1 to 5428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station
            19, Lausanne 1015, Switzerland"
FEATURES             Location/Qualifiers
     source          1..5428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    46..179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(216..232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(240..256)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(264..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(309..330)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(618..1203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1377..2234)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2235..2339)
                     /label=AmpR promoter
     misc_feature    2408..2611
                     /label=SV40 early promoter
                     /note="SV40 early promoter"
     promoter        2661..2679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2708..2713
                     /label=Kozak+ATG
                     /note="Kozak+ATG"
     CDS             2711..3361
                     /codon_start=1
                     /transl_table=11
                     /product="truncated DcuS"
                     /label=truncated DcuS
                     /protein_id="QKE44357.1"
                     /translation="MTSYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNY
                     QEEIGSLLGKIKSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLG
                     NLIENALEALGPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSE
                     RGVGLALVKQQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNRASASGSTGV"
     misc_feature    2720..3331
                     /label=DucS 340+
                     /note="DucS 340+"
     polyA_signal    3398..3622
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3668..4096
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4110..4439
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4506..5297
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
       61 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
      181 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
      241 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      301 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      361 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      421 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1141 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     2041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     2101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     2161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     2221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctcca cgcgtggtac
     2401 cctcgaggcg atctgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2461 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatcgctg actaattttt
     2521 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2581 ggcttttttg gaggcctagg cttttgcaaa agagctctct ggctaactag agaacccact
     2641 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tgttcgaagc
     2701 ggaattcacc atgactagtt atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     2761 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     2821 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     2881 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     2941 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     3001 gacgctgatt accacgttgg gaaatctgat agaaaacgcg ctggaggcat tagggccgga
     3061 acccggaggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgtgaagt
     3121 taatgatgat ggaccgggga tcgcacccga taaaatcgat cacatttttg acaaaggtgt
     3181 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     3241 tctcggcggc agcatcgccg tggaatcgga acccgggatt ttcacacaat tttttgtcca
     3301 gataccctgg gacggggaga ggtcgaacag agctagtgct agcggatcca ccggtgtcta
     3361 gaaagctttg aggccggcct gcagggatca gcctcgactg tgccttctag ttgccagcca
     3421 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3481 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3541 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3601 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3661 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3841 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3901 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3961 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4021 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4081 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4141 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4201 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4261 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4321 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4381 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4441 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     4501 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     4561 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     4621 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     4681 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     4741 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     4801 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     4861 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     4921 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     4981 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5041 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5101 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5161 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5221 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5281 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5341 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     5401 aatcgttttc cgggacgccg gctggatg
//
