LOCUS       V015740                 5815 bp    DNA     circular SYN 30-JUN-2020
DEFINITION  Exported.
ACCESSION   V015740
VERSION     V015740
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Scheller L, Schmollack M, Bertschi A, Mansouri M, Saxena P,
            Fussenegger M.
  TITLE     Phosphoregulated orthogonal signal transduction in mammalian cells
  JOURNAL   Nat Commun 11 (1), 3085 (2020)
   PUBMED   32555187
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Scheller L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment
            AI), Station 19, Lausanne 1015, Switzerland
REFERENCE   3  (bases 1 to 5815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Commun"; date: "2020"; volume: "11"; issue: "1"; pages: "3085"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-MAR-2020) STI IBI-STI LPDI, EPFL, AI 3123 (Batiment AI), Station
            19, Lausanne 1015, Switzerland"
FEATURES             Location/Qualifiers
     source          1..5815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..231
                     /label="SV40 early promoter"
                     /note="SV40 early promoter"
     promoter        281..299
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    328..333
                     /label="Kozak+ATG"
                     /note="Kozak+ATG"
     CDS             352..1068
                     /gene="dcuR"
                     /label="Transcriptional regulatory protein DcuR"
                     /note="Transcriptional regulatory protein DcuR from
                     Escherichia coli (strain K12). Accession#: P0AD01"
     CDS             1105..1338
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     polyA_signal    1405..1629
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1675..2103
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2117..2446
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2513..3304
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3481..3614
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3651..3667)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3675..3691)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3699..3729)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3744..3765)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4053..4638)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4812..5669)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5670..5774)
                     /label="AmpR promoter"
ORIGIN
        1 gagatctcca cgcgtggtac cctcgaggcg atctgcatct caattagtca gcaaccatag
       61 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      121 cccatcgctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      181 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa agagctctct
      241 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      301 agacccaagc tgttcgaagc ggaattcacc atgactagtg gtggttctgg tatgatcaat
      361 gtattaatta tcgatgacga cgcaatggtc gcggagctga atcgccgata cgtagcacaa
      421 atcccaggct ttcaatgctg tggaacagcc tcgacgctgg agaaagccaa agagattatc
      481 ttcaatagcg atacgcctat cgacctgata ttgctcgata tctatatgca aaaagagaac
      541 gggctcgatt tactgcctgt cctgcataac gcgcgttgca aaagtgatgt gattgtcatc
      601 tcctccgcag ccgatgcggc aaccattaaa gattcgctgc attacggtgt cgtggattac
      661 ctgatcaaac ccttccaggc ttcccgcttt gaagaggcgc tcaccggctg gcggcaaaag
      721 aaaatggcgc tggaaaaaca tcagtattat gaccaggccg agcttgatca gctaatccac
      781 ggcagcagct ccaacgaaca ggatcctcgt cgcttgccaa aaggcttaac gccgcagacg
      841 ttacgcacgc tgtgccagtg gattgacgcg catcaggact atgagttttc aaccgacgag
      901 ctagccaacg aggttaacat ttcgcgtgtt tcctgccgta aatacctcat ctggttggtc
      961 aactgccaca tcttgttcac cagtatccat tatggcgtca cggggcgtcc agtttaccgt
     1021 taccgtattc aggcagaaca ctactcacta ctgaaacaat attgccaagc tagcggatcc
     1081 accggttcta gtggtggttc tggtgccccc ccgaccgatg tcagcctggg ggacgagctc
     1141 cacttagacg gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg
     1201 gacatgttgg gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc
     1261 tacggcgctc tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga
     1321 attgacgagt acggtggggc tagtgctagc ggatccaccg gtgtctagaa agctttgagg
     1381 ccggcctgca gggatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     1441 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     1501 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     1561 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     1621 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     1681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1741 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1861 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1981 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2041 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2101 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2161 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2221 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2281 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2341 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     2401 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     2461 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     2521 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     2581 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     2641 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     2701 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     2761 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     2821 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     2881 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     2941 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3001 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     3061 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3121 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3181 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     3241 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     3301 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     3361 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     3421 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     3481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3541 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3601 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     3661 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3721 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3781 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3841 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3901 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3961 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4021 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4081 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4141 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4201 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4261 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4321 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4381 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4441 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4501 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4561 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
     4621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgg
//
