LOCUS       Exported                8343 bp DNA     circular SYN 15-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8343)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8343)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3868..3873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    342..699
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             764..1180
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1190..1564
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             1585..2301
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2725..3399
                     /codon_start=1
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    3856..4448
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3868..3873
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4449..5045
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             5150..5741
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(6042..6630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6797..7654)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7655..7759)
                     /label=AmpR promoter
     enhancer        7947..8326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
       61 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      121 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      181 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      241 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      301 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      361 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      421 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
      481 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
      541 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
      601 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
      661 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
      721 ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga ctgttaccac
      781 tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc
      841 ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa
      901 cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa
      961 ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga
     1021 agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc
     1081 tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg
     1141 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
     1201 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
     1261 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
     1321 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
     1381 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
     1441 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
     1501 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
     1561 actgccggat ctcccgccgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     1621 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1681 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1741 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1801 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1861 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1921 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1981 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     2041 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     2101 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     2161 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     2221 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2281 ggcatggacg agctgtacaa gggtggcggt ggctcgggcg gtggtgggtc gggtggcggc
     2341 ggatctatcg atgaattgaa ttccatgccg tccgagaaga ccttcaaaca gcgccggagc
     2401 ttcgaacaaa gagtggaaga tgtccggctc atccgggagc agcaccccac caagatccca
     2461 gtgattatag agcgatacaa gggtgagaag cagctgcccg tcctggacaa gaccaagttc
     2521 cttgtacctg atcacgtgaa tatgagcgaa ctcatcaaga taattagaag gcgcctgcag
     2581 ctcaatgcta accaagcctt cttcctcctg gtgaatgggc acagcatggt gagtgtgtcc
     2641 acacccatct ctgaagtgta cgagagcgag agagatgaag acggcttcct gtacatggtc
     2701 tatgcctccc aggagacgtt cgggatggcc tcctccgagg acgtcatcaa ggagttcatg
     2761 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2821 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     2881 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac
     2941 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3001 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3061 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3121 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc
     3181 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac
     3241 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc
     3301 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     3361 cagtacgagc gcgccgaggg ccgccactcc accggcgcca tcgatgaatt gaattccatg
     3421 ccgtccgaga agaccttcaa acagcgccgg agcttcgaac aaagagtgga agatgtccgg
     3481 ctcatccggg agcagcaccc caccaagatc ccagtgatta tagagcgata caagggtgag
     3541 aagcagctgc ccgtcctgga caagaccaag ttccttgtac ctgatcacgt gaatatgagc
     3601 gaactcatca agataattag aaggcgcctg cagctcaatg ctaaccaagc cttcttcctc
     3661 ctggtgaatg ggcacagcat ggtgagtgtg tccacaccca tctctgaagt gtacgagagc
     3721 gagagagatg aagacggctt cctgtacatg gtctatgcct cccaggagac gttctaagga
     3781 tcccagtgtg gtggtacggg aattcctgca ggcctcgagg gccggcgcgc cgcggccgct
     3841 acgtaaattc cgcccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag
     3901 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     3961 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4021 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4081 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4141 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4201 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4261 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4321 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4381 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4441 ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     4501 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4561 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4621 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     4681 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4741 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4801 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     4861 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4921 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     4981 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5041 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     5101 gctcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     5161 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
     5221 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     5281 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     5341 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     5401 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     5461 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     5521 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     5581 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     5641 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     5701 ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa
     5761 ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc
     5821 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5881 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5941 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     6001 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     6061 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     6121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     6181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     6241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6421 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6481 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6661 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6721 atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa
     6781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7081 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7381 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     7441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7741 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     7801 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcact
     7861 cgaggagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
     7921 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     7981 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8041 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8101 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     8161 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     8221 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     8281 tcctacttgg cagtacatct acgtattagt catcgctatt accatgctag agaaccatca
     8341 gat
//
