LOCUS       V004466                 5655 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004466
VERSION     V004466
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Xie W, Yu H.
  TITLE     Developing a sequential repressing-inducing control strategy for
            improving carotenoids production in Saccharomyces cerevisiae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Xie W, Yu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2014) Department of Chemical and Biological
            Engineering, Zhejiang University, Institute of Bioengineering, 34
            TianMuShan Road, Hangzhou, Zhejiang 310028, China
REFERENCE   3  (bases 1 to 5655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-FEB-2014) Department of Chemical and Biological Engineering,
            Zhejiang University, Institute of Bioengineering, 34 TianMuShan
            Road, Hangzhou, Zhejiang 310028, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(202..771)
                     /gene="PTH1"
                     /label="Peptidyl-tRNA hydrolase"
                     /note="Peptidyl-tRNA hydrolase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P38876"
     misc_feature    complement(796..1499)
                     /label="ERG9 partial CDS region"
                     /note="ERG9 partial CDS region"
     regulatory      complement(1497..2613)
                     /label="HXT1 promoter derived from S. cerevisiae"
                     /note="HXT1 promoter derived from S. cerevisiae"
                     /regulatory_class="promoter"
     promoter        2681..3088
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        3109..3127
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3168..3357
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(3370..3388)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3411..3444
                     /label="Loxp site"
                     /note="Loxp site"
     protein_bind    complement(3411..3444)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     gene            3490..4846
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     rep_origin      complement(4979..5567)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggatcttcga gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
       61 gatatcagat ccacggatca ctaaagggaa caaaagctgg agctggcctt gtaggccgcc
      121 ttccgatgca aagtgcagtg gaaaagaaaa agggcaaagc aaataggatg gtaagtcggt
      181 attgttgttg aagatgggct atgaaatgta ctgagtcaga gcacgccagg cagcaggttc
      241 actctgtgta agcaaggttt gtagttcctg cggagttaga gctcccagaa cccaccggga
      301 cacgctcgca gggtctctag aacgggaccc aggttctctg ccgattccaa tagccaattt
      361 ggcaaagggt acacggcctc cactgcattt tagcaggctt cgcagcccat tatgacctct
      421 aatactggtg ctgggggctc tgagctgcac ttttccacac gccacactta actcgtcatg
      481 aataactacg tgtcgggctt ggtacttggc gtaccgggcc cagaccttgc acacctgttc
      541 tccacttaga ttcatgtact ggccatccga tcttagtatc gtacaatgtt cgtcttctat
      601 ataatgcact ttgccgcccg tattaggcac aggggaataa gttctcccct gcagaccaag
      661 ccgctttcgt agcagctcca gcatatatag ccctacattg tgacgggtac cagcgtactg
      721 aggctctgga ttgcctatcc cggtcagcac tagtctccat ttaccggaca tcttccttat
      781 ctgcctagag tcgaccaaat cttcattgta atctctgatg atgttggttt tttgtaggaa
      841 aagacccatg ctttcataca attgctcatt agaatacaaa gattcgttgg caaacttggc
      901 aatgacaatc aaacgggtca aaccatcacc gaccaaacca gctacgtagt gacagtacac
      961 gtcgtagtcg tggacggttt gcaacccatt caagttgtaa ttttcatcta agatgtagtc
     1021 ggccatacca ttacccattt tctcggtgat ctccttgatg acttcttgat attctggttt
     1081 caatttgtgg aattcaataa gaatcgattc gaaatctgtc aaaacggctc tgtccttcac
     1141 atcgggggca tttccgtcga aactccattt agttaacaac aatttctcgt ggaagtgacg
     1201 caacaagtca attttcaaat cgtgttcgat ggacatatcg tcttcgatgg tatccaaagc
     1261 ccttaaaatc aaataaaaga gagtaacaca gtttctcaat tctggatgca gctctctgat
     1321 cacagcagca aacgatctgg aggtcaagtt caacagttcg aaacagtgca agagatatgg
     1381 agacgtggac tgatcataga tggagaatag cggtgttctg caaaacttca gcttcaaagc
     1441 tgccttcatc tcgaccggat gcaatgccaa ttgtaatagc tttcccatgc ggccgcgatt
     1501 ttacgtatat caactagttg acgattatga tatctttata gattttaagg tggggaaaga
     1561 acatgagacc ccagatggaa ttgattatgg ggacattgtt gcctttatat ataatttcaa
     1621 tatactaatt caaatgatta aaaacgtgag ggggacacgc aacttcgggt gttaagaaat
     1681 attttgctac attagataat ggtggagttt cctggcttgt cggataaaag ccatcaaatg
     1741 tcgcagcagc tcatgtttac gtttgctgtc ttctgcccac gtcatatgag tggtattctt
     1801 ctatcagcac ttgatgaata ttctttttct catatatctg aaagacaaaa gatcggcacg
     1861 gcaatgccct gcagcatttc ttcctagttt ttccgaattt ccattacgta ttggatcttg
     1921 tgcgcatatt tgtcagtcct tcacggaaaa aaaaaaagag cactgggtca cttcggaaaa
     1981 acttttgact caatgcaaca gtgtcataat cctttgcgct gtctctttga agaaaaatca
     2041 ggagtgcaag atatcgatta attccttgga agttatgatg gttagtctta gtttaactct
     2101 cttgaagaag ggttttttca gttggtcaac actctttaga ggtaaaaaaa aaaaaaaaaa
     2161 aaaaaaaaag agaattcttc atgtaattta ccatgattct acgtttttgc aagcaaaaat
     2221 gaagataatc cgagcgcatg cgcaagtagt ccctgccatg ccgcttcgga aaaactttcg
     2281 aaaccaatac tccttacatc cgccttgaat ttaggaccta caattgttgt ctcttctgct
     2341 tgaaccaata agccctagaa accttacacc ctaatttgca caagaaaact acgaaaaaat
     2401 tttttcctct tttttccgca gctccaatct cgccctgcta tttttttggt tcggagaaaa
     2461 atagtcggtc gatatgaatt gaagtttcat tgtggccact cgtttaggaa tggaataacc
     2521 tccgcgaatg gaaaaaaaga actcggaata tcaaaaattc cggctcaaag gaaaaatttg
     2581 cttcaggagg ggggaattat attccagatg agacctgcat acccctcatt tccacgaccg
     2641 cgaatcctta catcacaccc aatcccccac aagtgatccc ccacacacca tagcttcaaa
     2701 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
     2761 cttcaaaaca cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt
     2821 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
     2881 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaaatt tttttttttg
     2941 atttttttct ctttcgatga cctcccattg atatttaagt taataaacgg tcttcaattt
     3001 ctcaagtttc agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta
     3061 gaaagaaagc atagcaatct aatctaagtt ttaattacaa aggatccgta atacgactca
     3121 ctatagggcc cgggcgtcga gtaagcttgg taccgcggct agctaagatc cgctctaacc
     3181 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     3241 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     3301 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaagatc
     3361 ccaattcgcc ctatagtgag tcgtattacg cgcgctcgac aacccttaat ataacttcgt
     3421 ataatgtatg ctatacgaag ttattaggtc tagtagcttg cctcgtcccc gccgggtcac
     3481 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
     3541 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
     3601 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag
     3661 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
     3721 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
     3781 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatgggta
     3841 aggaaaagac tcacgtttcg aggccgcgat taaattccaa catggatgct gatttatatg
     3901 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     3961 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     4021 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     4081 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggcaaaa
     4141 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     4201 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     4261 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     4321 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
     4381 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     4441 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     4501 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     4561 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     4621 tgatgctcga tgagtttttc taatcagtac tgacaataaa aagattcttg ttttcaagaa
     4681 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     4741 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     4801 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     4861 ctaacgccgc catccagtgt cgaaaacgaa cagaatcagg ggataacgca ggaaagaaca
     4921 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4981 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5041 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5101 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5161 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5221 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5281 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5341 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5401 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5461 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5521 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5581 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5641 tgagattatc aaaaa
//
