LOCUS       40924_31990        9697 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMQ123i, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Shanks RM, Kadouri DE, MacEachran DP, O'Toole GA.
  TITLE     New yeast recombineering tools for bacteria
  JOURNAL   Plasmid 62 (2), 88-97 (2009)
  PUBMED    19477196
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Shanks RM, MacEachran DP, Powers SZ, Kadouri DE, O'Toole GA.
  TITLE     In vivo recombination vectors for modification and expression of 
            genes in Gram-negative and Gram-positive bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9697)
  AUTHORS   Shanks RM, MacEachran DP, Powers SZ, Kadouri DE, O'Toole GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2008) Ophthalmology, University of Pittsburgh, 203
            Lothrop St., Pittsburgh, PA 15213, USA
REFERENCE   4  (bases 1 to 9697)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "62"; issue: "2"; pages: "88-97"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (29-FEB-2008) Ophthalmology, University of Pittsburgh, 203 Lothrop 
            St., Pittsburgh, PA 15213, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..801
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     oriT            complement(893..1001)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1198..1728
                     /label=oriV
                     /note="incP origin of replication"
     CDS             2120..3265
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     terminator      complement(3525..3611)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3841..4554)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    complement(4653..4669)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4677..4705)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     promoter        4935..5012
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5136..5984
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI"
                     /label=lacI
                     /note="lac repressor"
                     /protein_id="ACB45176.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARRWRAISCCPSH
                     W"
     gene            5136..5984
                     /gene="lacI"
                     /label=lacI
     rep_origin      complement(6247..6835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7047..7577)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(7766..7794)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      complement(7871..9213)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        9477..9697
                     /label=URA3 promoter
ORIGIN
        1 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
       61 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
      121 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
      181 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
      241 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
      301 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
      361 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
      421 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
      481 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
      541 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
      601 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg
      661 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag
      721 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
      781 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
      841 tcacaaatta gagcttcaat ttaattatat cagttattca cgtggagctt atcggccagc
      901 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
      961 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
     1021 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     1081 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaagtatac
     1141 cttaaccgcc ggctgacccg gcggggacga ggcaagctaa acagatctct agacctaggc
     1201 cgccggcgtt gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac
     1261 gttgacactt gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga
     1321 tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac
     1381 gcgagtttcc cacagatgac gtggacaagc ctggggataa gtgccctgcg gtattgacac
     1441 ttgaggggcg cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt
     1501 gatgacagat gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc
     1561 agcatttgca agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct
     1621 tttaaaccaa tatttataaa ccttgttttt aaccagggct gcgccctggc gcgtgaccgc
     1681 gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa cgcggcaccc
     1741 catcccccca ggggctgcgc ccctcggccg cgaacgacct caccccaaaa atggcagcca
     1801 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     1861 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     1921 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     1981 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     2041 ggatctgatg gcgcagggga tcaagatcga cggatcgatc cggggaatta attccggggc
     2101 aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg aaggcataca ggcaagaact
     2161 gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg
     2221 tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc cagcaagcta cggccaagat
     2281 cgagcgcgac agcgtgcaac tggctccccc tgccctgccc gcgccatcgg ccgccgtgga
     2341 gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg aagtcgatga ccatcgacac
     2401 gcgaggaact atgacgacca agaagcgaaa aaccgccggc gaggacctgg caaaacaggt
     2461 cagcgaggcc aagcaggccg cgttgctgaa acacacgaag cagcagatca aggaaatgca
     2521 gctttccttg ttcgatattg cgccgtggcc ggacacgatg cgagcgatgc caaacgacac
     2581 ggcccgctct gccctgttca ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa
     2641 caaggtcatt ttccacgtca acaaggacgt gaagatcacc tacaccggcg tcgagctgcg
     2701 ggccgacgat gacgaactgg tgtggcagca ggtgttggag tacgcgaagc gcacccctat
     2761 cggcgagccg atcaccttca cgttctacga gctttgccag gacctgggct ggtcgatcaa
     2821 tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg
     2881 cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt
     2941 cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt
     3001 gctgtttgct ggcgaccact acacgaaatt catatgggag aagtaccgca agctgtcgcc
     3061 gacggcccga cggatgttcg actatttcag ctcgcaccgg gagccgtacc cgctcaagct
     3121 ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca
     3181 ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg gtggaacacg cctgggtcaa
     3241 tgatgacctg gtgcattgca aacgctaggg ccttgtgggg tcagttccgg ctgggggttc
     3301 agcagccacc tgcattaatt aactagaggc cgtggccacc acggcccggc ctgcctttca
     3361 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3421 cgaaaggggg atgtgctgca aggatgcctg gcagtttatg gcgggcgtcc tgcccgccac
     3481 cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg
     3541 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     3601 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc
     3661 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact
     3721 gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag
     3781 gctgaaaatc ttctctcatc cgccaaaaca gccaagcttg catgcctgca gactagtcta
     3841 tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
     3901 catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
     3961 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
     4021 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
     4081 cttttgtttg tctgccatga tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg
     4141 tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc tattaacaag
     4201 ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa
     4261 tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt catgctgttt
     4321 catatgatct gggtatctcg caaagcattg aacaccataa ccgaaagtag tgacaagtgt
     4381 tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa gttttccgta
     4441 tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa catcaccatc
     4501 taattcaaca agaattggga caactccagt gaaaagttct tctcctttac tcatatgtat
     4561 atctccttcc ccgggtaccg agctcgaatt cgctagccca aaaaaacggg tatggagaat
     4621 ctagaggatc caattctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt
     4681 atacgagccg atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg
     4741 atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc
     4801 agtgatttac gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc
     4861 attggtgcac cgtgcagtcg ataagcccgg atcagcttgc aattcgcgcg cgaaggcgaa
     4921 gcggcattta cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc
     4981 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
     5041 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
     5101 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
     5161 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
     5221 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
     5281 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
     5341 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
     5401 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
     5461 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
     5521 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
     5581 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
     5641 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
     5701 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
     5761 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     5821 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     5881 aacaggattt tcgcctgctg gggcaaacca cgtggaccgc ttgctgcaac tctctcaggg
     5941 ccaggcggtg gagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct
     6001 ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6061 acgacaggtt tcccgactgg aaagcgggca gtgagcctcg gtcgttcggc tgcggcgagc
     6121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6661 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6781 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6841 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6901 tttggtcatg agattatcaa aaaggatctt cacctagata tgagtaaact tggtctgaca
     6961 atcgatgcga attggccgcg gcgttgtgac aatttaccga acaactccgc ggccgggaag
     7021 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     7081 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     7141 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     7201 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     7261 atctcactac gcggctgctc aaacctgggc agaacgtaag ccgcgagagc gccaacaacc
     7321 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     7381 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     7441 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     7501 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     7561 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     7621 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     7681 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     7741 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaattcggt
     7801 tgaatactca tactcttcaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7861 gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc
     7921 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa
     7981 agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg
     8041 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     8101 gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca
     8161 tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg
     8221 ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag
     8281 gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta
     8341 ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt
     8401 ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt
     8461 cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga
     8521 aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt
     8581 tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc
     8641 aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat
     8701 agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct
     8761 cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt
     8821 tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt
     8881 caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct
     8941 cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa
     9001 cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga
     9061 aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc
     9121 ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc
     9181 cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac
     9241 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     9301 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     9361 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     9421 gcttaactat gcggcatcag agcagattgt actgagagtg caccatacca cagcttttca
     9481 attcaattca tcattttttt tttattcttt tttttgattt cggtttcttt gaaatttttt
     9541 tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac ttagattggt
     9601 atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct taacccaact
     9661 gcacagaaca aaaacctgca ggaaacgaag ataaatc
//
