LOCUS       62056_17240        4266 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    46..170
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        207..237
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             291..947
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1292..1594
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1638..1762)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    complement(1820..1941)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2360..2948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3151..4008)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4113..4161
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4175..4266
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacca gtgtggtgga attctgcaga tatcaacaag tttgtacaaa
       61 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
      121 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggcccctc
      181 gaggggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      241 ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa
      301 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      361 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      421 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      481 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      541 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      601 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      661 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      721 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      781 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
      841 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
      901 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat
      961 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
     1021 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg
     1081 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     1141 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     1201 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     1261 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag
     1321 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     1381 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     1441 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     1501 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     1561 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     1621 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct
     1681 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     1741 cagctttctt gtacaaagtg gttggcctcg gcggccaagc tctagagtcg gggcggccgg
     1801 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1861 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1921 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1981 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     2041 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2101 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2161 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2221 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2281 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2341 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2401 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2461 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2521 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2581 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2641 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2701 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2761 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2821 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2881 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2941 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3001 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3061 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     3121 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3181 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3241 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3301 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3361 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     3421 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     3481 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     3541 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     3601 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     3661 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     3721 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     3781 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     3841 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     3901 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     3961 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4021 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     4081 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     4141 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     4201 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4261 ttctct
//
