LOCUS       V004677                 9808 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004677
VERSION     V004677
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(360..384)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     CDS             1760..1948
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2053..2193
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2379..2967)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    3498..4386
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             3540..4328
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    4637..4661
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     terminator      complement(4908..5549)
                     /label="E9 terminator"
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
     CDS             complement(5559..6923)
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     misc_feature    complement(6924..7151)
                     /label="derived from A. thaliana ShkG"
                     /note="derived from A. thaliana ShkG"
     5'UTR           complement(7161..7828)
                     /label="derived from A. thaliana Tsf1"
                     /note="derived from A. thaliana Tsf1"
     regulatory      complement(7829..8308)
                     /label="derived from A. thaliana Tsf1"
                     /note="derived from A. thaliana Tsf1"
                     /regulatory_class="promoter"
     regulatory      complement(8332..8868)
                     /label="FMV 35S"
                     /note="FMV 35S"
                     /regulatory_class="enhancer"
     misc_feature    8931..8955
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(9711..9735)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tcgagcctgc aggatcctca gcagtcgctg tgcgatcgcc agcggtactc gctgaggatt
       61 taaataagcg gccgcggcgc gccaaatcgt gaagtttctc atctaagccc ccatttggac
      121 gtgaatgtag acacgtcgaa ataaagattt ccgaattaga ataatttgtt tattgctttc
      181 gcctataaat acgacggatc gtaatttgtc gttttatcaa aatgtacttt cattttataa
      241 taacgctgcg gacatctaca tttttgaatt gaaaaaaaat tggtaattac tctttctttt
      301 tctccatatt gaccatcata ctcattgctg atccatgtag atttcccgga catgaagcca
      361 tttacaattg aatatatcct gccgccgctg ccgctttgca cccggtggag cttgcatgtt
      421 ggtttctacg cagaactgag ccggttaggc agataatttc cattgagaac tgagccatgt
      481 gcaccttccc cccaacacgg tgagcgacgg ggcaacggag tgatccacat gggacttttc
      541 ctagcttggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
      601 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
      661 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
      721 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
      781 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
      841 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
      901 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
      961 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
     1021 ggccgaaatc gagcctgccc ctcatctgtc gcgccgccct ataccttgtc tgcctccccg
     1081 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     1141 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     1201 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     1261 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     1321 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     1381 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     1441 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     1501 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     1561 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     1621 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     1681 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     1741 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     1801 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
     1861 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
     1921 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     1981 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2041 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     2101 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2161 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     2221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3361 ttatccgcct ccatccagtc tattaattgt tgtcgggaag ctagagtaag tagttcgcca
     3421 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggtc gggagcacag gatgacgcct
     3481 aacaattcat tcaagccgac accgcttcgc ggcgcggctt aattcaggag ttaaacatca
     3541 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     3601 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     3661 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     3721 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     3781 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     3841 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     3901 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     3961 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     4021 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     4081 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     4141 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     4201 agcccgtcat acttgaagct aggcaggctt atcttggaca agaagatcgc ttggcctcgc
     4261 gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc aaggtagtcg
     4321 gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa
     4381 gcgttagatg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4441 tccggttccc aacgatcaag gcgagttaca tgatcccgtg gaaaagtccc atgtggatca
     4501 ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg
     4561 gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg
     4621 tgcaaagcgg cagcggcggc aggatatatt caattgtaaa tggcttcatg tccgggaaat
     4681 ctacatggat cagcaatgag tatgatggtc aatatggaga aaaagaaaga gtaattacca
     4741 attttttttc aattcaaaaa tgtagatgtc cgcagcgtta ttataaaatg aaagtacatt
     4801 ttgataaaac gacaaattac gatccgtcgt atttataggc gaaagcaata aacaaattat
     4861 tctaattcgg aaatctttat ttcgacgtgt ctgtggctgc aggtcgattg atgcatgttg
     4921 tcaatcaatt ggcaagtcat aaaatgcatt aaaaaatatt ttcatactca actacaaatc
     4981 catgagtata actataatta taaagcaatg attagaatct gacaaggatt ctggaaaatt
     5041 acataaagga aagttcataa atgtctaaaa cacaagagga catacttgta ttcagtaaca
     5101 tttgcagctt ttctaggtct gaaaatatat ttgttgccta gtgaataagc ataatggtac
     5161 aactacaagt gttttactcc tcatattaac ttcggtcatt agaggccacg atttgacaca
     5221 tttttactca aaacaaaatg tttgcatatc tcttataatt tcaaattcaa cacacaacaa
     5281 ataagagaaa aaacaaataa tattaatttg agaatgaaca aaaggaccat atcattcatt
     5341 aactcttctc catccatttc catttcacag ttcgatagcg aaaaccgaat aaaaaacaca
     5401 gtaaattaca agcacaacaa atggtacaag aaaaacagtt ttcccaatgc cataatactc
     5461 aaactcagta ggattctggt gtgtgcgcaa tgaaactgat gcattgaact tgacgaacgt
     5521 tgtcgaaacc gatgatacga acgaaagctg gctcatcaag cagccttagt gtcggagagt
     5581 tcgatcttag ctccaagacc agccatcaaa tccatgaact ctgggaagct agtagcgatc
     5641 atagtagcat catcaacagt aacagggttt tcagaaacga gacccataac gaggaagctc
     5701 atagcgatac ggtgatcgag gtgggtagcg acagctgctc cagaagcgtt accgagaccc
     5761 ttaccgtcag gacgaccacg cacgacgaga gaagtctcac cttcatcgca atcaacaccg
     5821 ttgagcttga gaccgtttgc gacagcagaa agacggtcgc tttccttaac acggagttct
     5881 tccaaaccgt tcataacggt agcaccttca gcgaatgcag ctgcaacagc gagaattgga
     5941 tactcgtcga tcatagaagg agcacggtct tctggaacag taacaccctt caaagtagaa
     6001 gaacgaacac gcaagtcagc cacgtcttct ccaccagcaa gacgtgggtt gatcacttcg
     6061 atgtcggcac ccatttcctg cagagtcaag atgagaccag tacgggttgg gttcatcaaa
     6121 acgttaagga tggtgacgtc ggaacctgga acaagcaagg cagcaaccaa tgggaaagca
     6181 gtagaggatg gatcacctgg aacatcaatc acttgaccgg tgagcttacc acgaccttca
     6241 agacggatgg tacgcacacc gtcagcatca gtctcaacgg taaggttagc accaaaacct
     6301 tgaagcatct tttcagtgtg gtcacgagtc atgattggct cgataacagt ggtgatacct
     6361 ggggtgttga gaccagcaag cagaacagcg gacttcactt gagcggaagc cataggtacc
     6421 ctgtaggtga ttggcgttgg agtctttggt ccacgcaagg taactggaag acgatcaccg
     6481 tcttcagact tcacctgcac acccatttcg cgaagtgggt tcaacacacg acccattgga
     6541 cgcttagtga gagaagcgtc accaatgaaa gtgctatcga aatcgtaaac accaacaaga
     6601 cccatagtca aacggcaacc agttgcagcg ttaccgaaat cgagaggagc ctcaggagca
     6661 aggagtccac cgttaccaac accatcaatg atccaagtat caccttcctt acggattctg
     6721 gcacccatag cttgcatagc cttaccagtg ttgataacat cttcaccttc caaaagaccg
     6781 gtgatacgag tttcaccgct agcgagacct ccaaacatga aggacctgtg ggagatagac
     6841 ttgtcacctg gaatacggac ggttccagaa agaccagagg acttacgagc agttgctgga
     6901 cggctgcttg caccgtgaag catgcacgcc gtggaaacag aagacatgac cttaagagga
     6961 cgaagctcag agccaattaa cgtcatccca ctcttcttca atccccacga cgacgaaatc
     7021 ggataagctc gtggatgctg ctgcgtcttc agagaaaccg ataagggaga tttgcgttga
     7081 ctggatttcg agagattgga gataagagat gggttctgca caccattgca gattctgcta
     7141 acttgcgcca tggtagttct ctggtcaaca aatctgcaaa aaaaagaaca atcaaatatt
     7201 gaatcaataa aaacagtaga catatataac cattatgaaa atcatcttta acacaaaaca
     7261 atcatagtaa aaagccgcta tcggagtcaa accgatttct accacaaaaa ttgtggtgct
     7321 ctatcatctg agctaagcga gctaaattca atcaaatcgg caatttcaag agatataaag
     7381 caaacaatcc gagaattacc cttagaaaaa gggaacactg gatttgttat gctactccag
     7441 aattacccaa tcacaattca aatcgacata catcttaaat acgcaaatca acaagttcac
     7501 tcgtatatag atccaccaac caacaatcaa tcgaacaaat taaaatctca aaacaagatc
     7561 gatcagaatg ggaaaatagt ttcatgaata aattatcgtt tagaagcagt gaaacgttga
     7621 tattaacaat cgataaaatc taaacaacag atcagaattt tcagaacgat caatcaacaa
     7681 agagcatcga atccaacaat catcgaaata atatgagcaa cgatcagcag acgaaaaaga
     7741 aaaagaacga tctaagggta cgttaacgct tacctcagag atatcgcaga aaagtgtgta
     7801 gcggctaggg tcaataagaa cgataaatgg aatcagcaac gagagtggtt ttatttatag
     7861 cgttgaatcc gaaaccaatg ttagggttta acataaaata atatcattct ttaaattacc
     7921 attatgcccc tgtttagccg tgaatactca atacctcttg ggttggttgt gttttatgaa
     7981 tgtttagtac tacatgttac aaacatcatt taatcacacc ttttaattaa ggttattcaa
     8041 gcaaatggtt atacatgttc gtctcccaaa aatttcattg attgtggacc atcaatctat
     8101 atgacactct tctctagagt aaatatttga tatctcaatg aatgcagatt atcaagagcc
     8161 atttatatca tttattcaaa caccaaataa aataatgaac aagtaaagta tatattaacc
     8221 tcaaaatctc atgacaatgg aaatggttta ttataacaac catatgtgtc ccattccacg
     8281 agcaacctcc ctcaagagag aaacttccga tatcaagctt gatatcgaat tcttccttac
     8341 aaacctttga ggatgggagt tccttcttgg ttttggcgat accaatttga ataaagtgat
     8401 atggctcgta ccttgttgat tgaacccaat ctggaatgct gctaaatcct gagctcaagc
     8461 taattctttt gtggtcgtca ctgcgttcgt catacgcatt agtgagtggg ctgtcaggac
     8521 agctcttttc cacgttattt tgttccccac ttgtactaga ggaatctgct ttatctttgc
     8581 aataaaggca aagatgcttt tggtaggtgc gcctaacaat tctgcaccat tccttttttg
     8641 tctggtcccc acaagccagc tgctcgatgt tgacaagatt actttcaaag atgcccacta
     8701 actttaagtc ttcggtggat gtctttttct gaaacttact gaccatgatg catgtgctgg
     8761 aacagtagtt tactttgatt gaagattctt cattgatctc ctgtagcttt tggctaatgg
     8821 tttggagact ctgtaccctg accttgttga ggctttggac tgagaattgg atctaagctt
     8881 gatggggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
     8941 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     9001 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     9061 cgggagtgct tggcattccg tgcgataatg acttctgttc aaccacccaa acgtcggaaa
     9121 gcctgacgac ggagcagcat tccaaaaaga tcccttggct cgtctgggtc ggctagaagg
     9181 tcgagtgggc tgctgtggct tgatccctca acgcggtcgc ggacgtagcg cagcgccgaa
     9241 aaatcctcga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9301 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9361 gacaccacga tgcctgcaga agctagcttc cacgggccgg ccttcggtcc tccgatcgag
     9421 gatttttcgg cgctgcgcta cgtccgcgac cgcgttgagg gatcaagcca cagcagccca
     9481 ctcgaccttc tagccgaccc agacgagcca agggatcttt ttggaatgct gctccgtcgt
     9541 caggctttcc gacgtttggg tggttgaaca gaagtcatta tcgcacggaa tgccaagcac
     9601 tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt
     9661 cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc
     9721 caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc
     9781 ccatcaagct agcttctgca ggtcctgc
//
