LOCUS       V004680                 9769 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004680
VERSION     V004680
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Preuss SB, Meister R, Xu Q, Urwin CP, Tripodi FA, Screen SE, Anil
            VS, Zhu S, Morrell JA, Liu G, Ratcliffe OJ, Reuber TL, Khanna R,
            Goldman BS, Bell E, Ziegler TE, McClerren AL, Ruff TG, Petracek ME.
  TITLE     Expression of the Arabidopsis thaliana BBX32 Gene in Soybean
            Increases Grain Yield
  JOURNAL   PLoS ONE 7 (2), E30717 (2012)
   PUBMED   22363475
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Preuss SB.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "2"; pages: "E30717"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUL-2011) Yield "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        271..615
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     3'UTR           688..1002
                     /label="derived from G. barbadense E6"
                     /note="derived from G. barbadense E6"
     misc_feature    1073..1097
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    complement(1526..2414)
                     /label="derived from E. coli aadA"
                     /note="derived from E. coli aadA"
     CDS             complement(1584..2372)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2945..3533
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3719..3859)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3964..4152)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      5420..6133
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    6299..6323
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     regulatory      6624..7223
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
                     /regulatory_class="promoter"
     5'UTR           7224..7861
                     /label="derived from A. thaliana Act7"
                     /note="derived from A. thaliana Act7"
     CDS             7864..8091
                     /codon_start=1
                     /product="EPSPS CTP"
                     /label="EPSPS CTP"
                     /note="derived from A. thaliana ShkG"
                     /protein_id="AEP17835.1"
                     /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI
                     SSSWGLKKSGMTLIGSELRPLKVMSSVSTAC"
     CDS             8092..9456
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     terminator      9466..9718
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 ggtccgatgt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
       61 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
      121 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      181 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      241 aaagcaagtg gattgatgtg atggtccgat tgagactttt caacaaaggg taatatccgg
      301 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa
      361 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc
      421 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga
      481 agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag
      541 ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt
      601 tcatttggag aggaccaggt ggtaccggcg cgcctcagca gtcgctgtcg ttaacccagc
      661 ggtactcgct gaggcgatcg cgggccctga tcacctgtcg tacagtattt ctacatttga
      721 tgtgtgattt gtgaagaaca tcaaacaaaa caagcactgg ctttaatatg atgataagta
      781 ttatggtaat taattaattg gcaaaaacaa caatgaagct aaaattttat ttattgagcc
      841 ttgcggttaa tttcttgtga tgatcttttt ttttattttc taattatata tagtttcctt
      901 tgctttgaaa tgctaaaggt ttgagagagt tatgctcttt ttttcttcct ctttcttttt
      961 taactttatc atacaaattt tgaataaaaa tgtgagtaca ttgagctcat ttaaataagc
     1021 ttgatgggga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
     1081 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
     1141 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
     1201 ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga
     1261 aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa
     1321 ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg
     1381 aaaaatcctc gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1441 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1501 gtgacaccac gatgcctgca gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt
     1561 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
     1621 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
     1681 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1741 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1801 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1861 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1921 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1981 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     2041 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     2101 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     2161 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     2221 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     2281 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2341 gtcgatactt cggcgatcac cgcttccctc atgatgttta actcctgaat taagccgcgc
     2401 cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat cctgtgctcc cgacctgcag
     2461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3721 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     3781 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     3841 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3901 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3961 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     4021 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     4081 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     4141 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
     4201 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
     4261 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
     4321 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
     4381 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
     4441 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
     4501 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
     4561 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
     4621 ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     4681 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     4741 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     4801 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     4861 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg
     4921 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt
     4981 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     5041 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     5101 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
     5161 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     5221 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     5281 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     5341 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     5401 aagggcatcg gtcgatcgac cctttccgac gctcaccggg ctggttgccc tcgccgctgg
     5461 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
     5521 acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     5581 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     5641 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     5701 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     5761 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     5821 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     5881 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     5941 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     6001 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     6061 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     6121 ccaaaaatgg cagccaagct aggaaaagtc ccatgtggat cactccgttg ccccgtcgct
     6181 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc
     6241 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggcg
     6301 gcaggatata ttcaattgta aatggcttca tgtccgggaa atctacatgg atcagcaatg
     6361 agtatgatgg tcaatatgga gaaaaagaaa gagtaattac caattttttt tcaattcaaa
     6421 aatgtagatg tccgcagcgt tattataaaa tgaaagtaca ttttgataaa acgacaaatt
     6481 acgatccgtc gtatttatag gcgaaagcaa taaacaaatt attctaattc ggaaatcttt
     6541 atttcgacgt gtctacattc acgtccaaat gggggcttag atgagaaact tcacgatcga
     6601 tgcggccacc actcgagaag cttactagtc aacaattggc caatctttgt tctaaattgc
     6661 taataaacga ccatttccgt caattctcct tggttgcaac agtctacccg tcaaatgttt
     6721 actaatttat aagtgtgaag tttgaattat gaaagacgaa atcgtattaa aaattcacaa
     6781 gaataaacaa ctccatagat tttcaaaaaa acagtcacga gaaaaaaacc acagtccgtt
     6841 tgtctgctct tctagttttt attatttttc tattaatagt tttttgttat ttcgagaata
     6901 aaatttgaac gatgtccgaa ccacaaaagc cgagccgata aatcctaagc cgagcctaac
     6961 tttagccgta accatcagtc acggctcccg ggctaattca tttgaaccga atcataatca
     7021 acggtttaga tcaaactcaa aacaatctaa cggcaacata gacgcgtcgg tgagctaaaa
     7081 agagtgtgaa agccaggtca ccatagcatt gtctctccca gattttttat ttgggaaata
     7141 atagaagaaa tagaaaaaaa taaaagagtg agaaaaatcg tagagctata tattcgcaca
     7201 tgtactcgtt tcgctttcct tagtgttagc tgctgccgct gttgtttctc ctccatttct
     7261 ctatctttct ctctcgctgc ttctcgaatc ttctgtatca tcttcttctt cttcaaggtg
     7321 agtctctaga tccgttcgct tgattttgct gctcgttagt cgttattgtt gattctctat
     7381 gccgatttcg ctagatctgt ttagcatgcg ttgtggtttt atgagaaaat ctttgttttg
     7441 ggggttgctt gttatgtgat tcgatccgtg cttgttggat cgatctgagt taattcttaa
     7501 ggtttatgtg ttagatctat ggagtttgag gattcttctc gcttctgtcg atctctcgct
     7561 gttatttttg tttttttcag tgaagtgaag ttgtttagtt cgaaatgact tcgtgtatgc
     7621 tcgattgatc tggttttaat cttcgatctg ttaggtgttg atgtttacaa gtgaattcta
     7681 gtgttttctc gttgagatct gtgaagtttg aacctagttt tctcaataat caacatatga
     7741 agcgatgttt gagtttcaat aaacgctgct aatcttcgaa actaagttgt gatctgattc
     7801 gtgtttactt catgagctta tccaattcat ttcggtttca ttttactttt tttttagtga
     7861 accatggcgc aagttagcag aatctgcaat ggtgtgcaga acccatctct tatctccaat
     7921 ctctcgaaat ccagtcaacg caaatctccc ttatcggttt ctctgaagac gcagcagcat
     7981 ccacgagctt atccgatttc gtcgtcgtgg ggattgaaga agagtgggat gacgttaatt
     8041 ggctctgagc ttcgtcctct taaggtcatg tcttctgttt ccacggcgtg catgcttcat
     8101 ggagcttcat ctaggccagc tactgccagg aagtctagcg ggctcagtgg caccgtgcgc
     8161 atccctggcg ataaaagtat ttcacacagg agcttcatgt tcggaggact tgctagtgga
     8221 gagacgagaa tcactggttt gcttgagggc gaagatgtta tcaacaccgg taaggcgatg
     8281 caagcaatgg gtgccagaat ccgaaaagag ggcgatacgt ggatcatcga cggtgttggt
     8341 aacggaggat tgctcgctcc cgaagcgcca cttgactttg ggaacgcagc tacggggtgc
     8401 cgtcttacta tgggactggt aggcgtgtat gactttgact ctaccttcat cggtgacgcg
     8461 agcctcacta agagaccaat gggacgagtg ctgaatcccc tgagggagat gggtgtccag
     8521 gtgaaatctg aggatggtga tcgtcttccg gttactctgc gaggccccaa gacccccacg
     8581 ccaatcacgt acagggttcc gatggcgtca gcacaggtca agtcagcggt actcctggcg
     8641 ggcctcaaca cacctggaat cacaaccgtg attgaaccca tcatgactag agaccacacg
     8701 gagaagatgt tgcagggttt cggcgctaat ctaacggtcg aaaccgacgc cgacggcgtg
     8761 aggacaatcc gcttggaggg cagaggtaaa ctgactggcc aagtcatcga tgtgcctgga
     8821 gatccctcgt ccacagcgtt tcccctcgta gctgcgttgc tcgtccctgg atctgatgtg
     8881 acgatcctga atgtcctcat gaatccaact agaaccggcc tcatcctcac attgcaggag
     8941 atgggtgctg acatcgaggt tatcaatcct aggttggcag gtggagagga tgtggccgat
     9001 ctgcgcgtgc gttctagtac actcaaaggc gtgaccgtcc ctgaggatcg cgctccatcc
     9061 atgatcgacg agtaccccat tctcgccgtt gctgctgcgt ttgccgaggg cgcaactgta
     9121 atgaacggcc ttgaggagtt gagggttaag gagagtgaca ggctgtccgc ggtggcgaat
     9181 ggcctgaagc taaacggcgt ggactgcgac gaaggtgaaa cgtcccttgt agtccgtggt
     9241 cgcccagacg ggaaggggtt ggggaatgct tcgggagctg ctgtggcgac gcaccttgat
     9301 catagaatcg ccatgtcatt tctggtgatg ggacttgtct ccgagaatcc ggtgaccgtt
     9361 gacgatgcta ccatgatcgc cacctccttt cctgagttca tggacctcat ggcaggcttg
     9421 ggggccaaga tcgagctgtc tgatactaag gccgcttgaa ttcccgatcg ttcaaacatt
     9481 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
     9541 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
     9601 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
     9661 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
     9721 ggatcccacg tgcggaccgc ctgcaggccg cgttatcaag ctaactgca
//
