LOCUS       40924_31135        4266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pMM25, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Moscariello MM, Sutherland BM.
  TITLE     Transferring linear DNA fragments in the yeast Saccharomyces 
            cerevisiae: A new in vivo eukaryotic system that reveals deleterious
            combinations of DNA cluster damage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Moscariello MM, Sutherland BM.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2009) Biology, Brookhaven National Laboratory, 50 
            Bell Avenue, Upton, NY 11973, USA
REFERENCE   3  (bases 1 to 4266)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2009) Biology, Brookhaven National Laboratory, 50 Bell 
            Avenue, Upton, NY 11973, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            713..1659
                     /gene="ADE2"
                     /label=ADE2
     regulatory      713..885
                     /gene="ADE2"
                     /regulatory_class="promoter"
     CDS             886..1659
                     /codon_start=1
                     /gene="ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /label=ADE2
                     /note="catalyzes a step in 'de novo' purine nucleotide 
                     biosynthetic pathway"
                     /protein_id="ACX71835.1"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMF"
     promoter        1812..1999
                     /label=HIS3 promoter
     CDS             2000..2018
                     /codon_start=1
                     /gene="HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /label=HIS3
                     /note="catalyzes the sixth step in histidine biosynthesis"
                     /protein_id="ACX71836.1"
                     /translation="MRQSRK"
     primer_bind     complement(2025..2041)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2078..2096)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2117..2133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2141..2157)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2165..2195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2210..2231)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2519..3107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3281..4138)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4139..4243)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccgggta
      721 atactaagtg attgactctt gctgaccttt tattaagaac taaatggaca atattatgga
      781 gcatttcatg tataaattgg tgcgtaaaat cgttggatct ctcttctaag tacatcctac
      841 tataacaatc aagaaaaaca agaaaatcgg acaaaacaat caagtatgga ttctagaaca
      901 gttggtatat taggaggggg acaattggga cgtatgattg ttgaggcagc aaacaggctc
      961 aacattaaga cggtaatact agatgctgaa aattctcctg ccaaacaaat aagcaactcc
     1021 aatgaccacg ttaatggctc cttttccaat cctcttgata tcgaaaaact agctgaaaaa
     1081 tgtgatgtgc taacgattga gattgagcat gttgatgttc ctacactaaa gaatcttcaa
     1141 gtaaaacatc ccaaattaaa aatttaccct tctccagaaa caatcagatt gatacaagac
     1201 aaatatattc aaaaagagca tttaatcaaa aatggtatag cagttaccca aagtgttcct
     1261 gtggaacaag ccagtgagac gtccctattg aatgttggaa gagatttggg ttttccattc
     1321 gtcttgaagt cgaggacttt ggcatacgat ggaagaggta acttcgttgt aaagaataag
     1381 gaaatgattc cggaagcttt ggaagtactg aaggatcgtc ctttgtacgc cgaaaaatgg
     1441 gcaccattta ctaaagaatt agcagtcatg attgtgagat ctgttaacgg tttagtgttt
     1501 tcttacccaa ttgtagagac tatccacaag gacaatattt gtgacttatg ttatgcgcct
     1561 gctagagttc cggactccgt tcaacttaag gcgaagttgt tggcagaaaa tgcaatcaaa
     1621 tcttttcccg gttgtggtat atttggtgtg gaaatgttca gattgtactg agagtgcacc
     1681 ataattccgt tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca
     1741 cggcattagt cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact
     1801 cttggcctcc tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt
     1861 tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga
     1921 attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc
     1981 aagataaacg aaggcaaaga tgcgacagag cagaaagccc gggggatcca ctagttctag
     2041 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     2101 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2161 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2221 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2281 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2341 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2401 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2461 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2521 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2581 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2641 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2701 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2761 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2821 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2881 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2941 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3001 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3061 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3241 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3301 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3361 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3421 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3481 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3541 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3601 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3661 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3721 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3781 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3841 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3901 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3961 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4021 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4081 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4141 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4201 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4261 tgccac
//
