LOCUS       40924_31105        6124 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector pMLS7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Lefebre MD, Valvano MA.
  TITLE     Construction and evaluation of plasmid vectors optimized for 
            constitutive and regulated gene expression in Burkholderia cepacia 
            complex isolates
  JOURNAL   Appl. Environ. Microbiol. 68 (12), 5956-5964 (2002)
  PUBMED    12450816
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Lefebre MD, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2002) Microbiology and Immunology, University of 
            Western Ontario, Dental Sciences Building Rm 3000, London, ONT N6A 
            5C1, Canada
REFERENCE   3  (bases 1 to 6124)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2002"; volume: "68"; issue: "12"; 
            pages: "5956-5964"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2002) Microbiology and Immunology, University of Western 
            Ontario, Dental Sciences Building Rm 3000, London, ONT N6A 5C1, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(836..938)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(1088..2107)
                     /codon_start=1
                     /gene="mob"
                     /product="Mob"
                     /function="required for plasmid mobilization"
                     /label=mob
                     /protein_id="AAM63386.1"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEHW
                     AASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKWLA
                     DKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAAAVA
                     DLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLGISKR
                     VETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGP
                     LNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSKKPLLS"
     gene            complement(1088..2107)
                     /gene="mob"
                     /label=mob
     rep_origin      2331..3100
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3101..3760
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     CDS             complement(4218..4451)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(4778..4806)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5069..5446
                     /gene="rpsL"
                     /label=rpsL
                     /note="Small ribosomal subunit protein uS12 from 
                     Paraburkholderia xenovorans (strain LB400). Accession#: 
                     Q13TG5"
     misc_feature    5642..5697
                     /note="multiple cloning site; EcoRI to HindIII"
     terminator      5900..5986
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6078..6105
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac
       61 gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc
      121 cgcgctcggc ctctctggcg gccttctggc gctgaggtct gcctcgtgaa gaaggtgttg
      181 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
      241 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
      301 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
      361 ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca
      421 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
      481 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
      541 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
      601 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gcgagaaatc
      661 accatgagtg acgactgaat ccggtgagaa tggcaaaagc taaaaaggcc gtaatatcca
      721 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      781 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      841 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      901 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
      961 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     1021 cttccgtcac aggtatttat tcgaagacga aagggcctcg tgatacgcct atttttatag
     1081 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1141 cgcgcccgcg ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg
     1201 tcagcagctt ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat
     1261 tcaacggccc cagggcgtcc agaacgggct tcaggcgctc ccgaaggtct cgggccgtct
     1321 cttgggcttg atcggccttc ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag
     1381 gctcataccc ctgccgaacc gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa
     1441 cgcgctttga gattcccagc ttttcggcca atccctgcgg tgcataggcg cgtggctcga
     1501 ccgcttgcgg gctgatggtg acgtggccca ctggtggccg ctccagggcc tcgtagaacg
     1561 cctgaatgcg cgtgtgacgt gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg
     1621 ccacagcggc cgcaaacgtg gtctggtcgc gggtcatctg cgctttgttg ccgatgaact
     1681 ccttggccga cagcctgccg tcctgcgtca gcggcaccac gaacgcggtc atgtgcgggc
     1741 tggtttcgtc acggtggatg ctggccgtca cgatgcgatc cgccccgtac ttgtccgcca
     1801 gccacttgtg cgccttctcg aagaacgccg cctgctgttc ttggctggcc gacttccacc
     1861 attccgggct ggccgtcatg acgtactcga ccgccaacac agcgtccttg cgccgcttct
     1921 ctggcagcaa ctcgcgcagt cggcccatcg cttcatcggt gctgctggcc gcccagtgct
     1981 cgttctctgg cgtcctgctg gcgtcagcgt tgggcgtctc gcgctcgcgg taggcgtgct
     2041 tgagactggc cgccacgttg cccattttcg ccagcttctt gcatcgcatg atcgcgtatg
     2101 ccgccatgcc tgcccctccc ttttggtgtc caaccggctc gacgggggca gcgcaaggcg
     2161 gtgcctccgg cgggccactc aatgcttgag tatactcact agactttgct tcgcaaagtc
     2221 gtgaccgcct acggcggctg cggcgcccta cgggcttgct ctccgggctt cgccctgcgc
     2281 ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg atggccgcga gcggccaccg
     2341 gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga caagctgatg gacaggctgc
     2401 gcctgcccac gagcttgacc acagggattg cccaccggct acccagcctt cgaccacata
     2461 cccaccggct ccaactgcgc ggcctgcggc cttgccccat caattttttt aattttctct
     2521 ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg ccggttggac accaagtgga
     2581 aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc gcctggaacg
     2641 acccaagcct atgcgagtgg gggcagtcga aggcgaagcc cgcccgcctg ccccccgagc
     2701 ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc accttgggca aggccgaagg
     2761 ccgcgcagtc gatcaacaag ccccggaggg gccacttttt gccggagggg gagccgcgcc
     2821 gaaggcgtgg gggaaccccg caggggtgcc cttctttggg caccaaagaa ctagatatag
     2881 ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa ggggggtacg caacagctca
     2941 ttgcggcacc ccccgcaata gctcattgcg taggttaaag aaaatctgta attgactgcc
     3001 acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa gttgcagctg attgcgcatg
     3061 gtgccgcaac cgtgcggcac cctaccgcat ggagataagc atggccacgc agtccagaga
     3121 aatcggcatt caagccaaga acaagcccgg tcactgggtg caaacggaac gcaaagcgca
     3181 tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg gcaatgctgc tgcatcacct
     3241 cgtggcgcag atgggccacc agaacgccgt ggtggtcagc cagaagacac tttccaagct
     3301 catcggacgt tctttgcgga cggtccaata cgcagtcaag gacttggtgg ccgagcgctg
     3361 gatctccgtc gtgaagctca acggccccgg caccgtgtcg gcctacgtgg tcaatgaccg
     3421 cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg gtgttcagtg ccgccgtggt
     3481 ggttgatcac gacgaccagg acgaatcgct gttggggcat ggcgacctgc gccgcatccc
     3541 gaccctgtat ccgggcgagc agcaactacc gaccggcccc ggcgaggagc cgcccagcca
     3601 gcccggcatt ccgggcatgg aaccagacct gccagccttg accgaaacgg aggaatggga
     3661 acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg tgttttctgg acgatggcga
     3721 gccgttggag ccgccgacac gggtcacgct gccgcgccgg tagcacttgg gttgcgcagc
     3781 aacccgtaag tgcgctgttc cagactatcg gctgtagccg cctcgccgcc ctataccttg
     3841 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
     3901 ccggcggcac ctcgctaacg gattcaccgt ttttatcagg ctctgggagg cagaataaat
     3961 gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc ctcaggcatt
     4021 tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac
     4081 ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa
     4141 tttctgccat tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc
     4201 accaataaca tcgcttaggc cacacgttca agtgcagcca caggataaat ttgcactgag
     4261 cctgggtggg attcggactc gaccgcatag ccttcaggag tgagttttgt gcaataccaa
     4321 ccgacgactt gaccctgcca agcggcacca gatttcttgc gtacgcgatc ccctaagcca
     4381 aaggtggcac tcaggggaag cgcaaactgc cctgcaacgg gagcgttggc ttcatcgcta
     4441 ctttgaccca tgtcgaatcc ttcttgtgaa tctattatgg cgacaagcaa atcgagctct
     4501 gactgcctac cccacaacaa ctactagaaa gcaccagcac aacggctgcc taactttgtt
     4561 ttagggcgac taccctgctg cgtaacatcg ttgctgttcc ataacatcaa acatcgaccc
     4621 acggcgtaac gcgcttagct agcttggatg cccgaggcta gactgtacaa aaaaacagtc
     4681 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     4741 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
     4801 tgtctataca gctagccgaa ttgctggaag tcgtcgccgc cgactaagcg ctcagcgagc
     4861 gcctggccag gtcggcaggg gtacgaaaca tcgcaacctg ttgactccct tagtaatttc
     4921 ggaatattat gctaggttcc ggttcttgga atgcctgcgc gcgaagtcta ttcgtgcgcc
     4981 gactaccggt ccggaagttc gatgcgcaat cgtttctgct ttgcccggaa acagatgcgt
     5041 cgattttgtt caatttcagg aataaacaat gccaaccatc aatcaactgg ttcgcaaagg
     5101 ccgcgcgtcg gaaacgacga agagcaagag cccggctttg caggactgcc cccagcgtcg
     5161 cggcgtgtgc acccgtgtgt acaccacgac gccgaagaag cctaactcgg cactgcgtaa
     5221 ggttgccaag gttcgtctga cgaacggctt cgaagtgatt tcttacatcg gtggtgaagg
     5281 ccacaatctg caggaacact cggtcgtgct gattcgcggc ggtcgtgtca aggacttgcc
     5341 gggtgtgcgt taccacatgg ttcgtggctc gctggatacc cagggcgtca aggatcgtaa
     5401 gcaggctcgc tcgaagtacg gtgcgaagcg tgcaaaagct ggtaaataag ctggcaacca
     5461 attagccggt cagtagtttc aggtcgcctg caaaggcggt aatagcggtg gtgccggaat
     5521 cgccggtgct gtcgagtaag tggtcacccg accaggctgg taagtcgtta gggatgaatc
     5581 gggttagttg gtggccgcgg ggctaaaagt tagctccaac tgaaaagtta aaggaagaaa
     5641 cgaattcacc atggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
     5701 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     5761 ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     5821 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     5881 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     5941 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     6001 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     6061 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     6121 tctt
//
