LOCUS       62056_17070        5570 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1736..1765
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1784..1807
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     primer_bind     complement(1827..1843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1854..2476
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     rep_origin      complement(2625..3213)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(3542..3589)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(3824..4615)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4650..5007)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(5009..5113)
                     /label=AmpR promoter
     rep_origin      5140..5570
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgtcga gcccgggcat
     1021 cgacggcgac cccaagcctc catgcttgcc tcgaaacggt ctggtgaagc tgccgggcca
     1081 gcccaacggc ctgggtgcgg ccagcatcac caagggcacg ccagccacca agaaccgccc
     1141 ctgccagcca ccacccccac ccaccctccc accacccagc ctggctgctc cactgtcccg
     1201 ggctgccctg gctgggggcc cgtgcacccc ggcaggtgga ccagcctcag ccttggcacc
     1261 tgggcaccca gcggagcggc cgccgctggc cacggacgag aagatcctca atgggctctt
     1321 ctggtatttc tcggcctgcg agaagtgtgt gctggcccag gtgtgcaagg cctggcggcg
     1381 cgtgctgtac cagcccaagt tctgggcagg cctcacgccg gtgctgcatg ccaaggagct
     1441 ctacaacgtg ctgcctggtg gcgagaagga gttcgtgaac ctgcagggtt ttgccgccag
     1501 aggcttcgag ggcttctgcc tggttggcgt ctccgacctg gacatctgtg agttcattga
     1561 caactatgcg ctctccaaga agggtgtcaa agccatgagc ctcaagcgct ccaccatcac
     1621 ggacgcaggc ctcgaggtta tgcttgaaca gatgcagggc gtggtgcgtc tggagctgtc
     1681 gggctgcaac gacttcaccg aggccgggct gtggtccagc ctgagcgcgc gcctcgagca
     1741 gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca aggatgacga
     1801 cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc
     1861 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     1921 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     1981 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     2041 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     2101 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     2161 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     2221 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     2281 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     2341 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     2401 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     2461 agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca ctcaaaggcg
     2521 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2581 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2641 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2701 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2761 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2821 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2881 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2941 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3001 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3061 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3121 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3181 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3241 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3301 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3361 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     3421 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     3481 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     3541 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     3601 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     3661 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     3721 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     3781 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     3841 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     3901 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     3961 gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4021 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4081 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4141 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     4201 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     4261 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     4321 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     4381 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     4441 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     4501 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     4561 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     4621 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     4681 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     4741 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     4801 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     4861 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     4921 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     4981 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     5041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
