LOCUS       V004711                 8267 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004711
VERSION     V004711
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
   PUBMED   15208639
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Kuo M-H., Xu X-J., Ng S-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 8267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages:
            "888-892"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309
            BCH Building, East Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             932..952
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             977..1894
                     /gene="KIN28"
                     /label="Serine/threonine-protein kinase KIN28"
                     /note="Serine/threonine-protein kinase KIN28 from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P06242"
     CDS             1928..1954
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1958..1984
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1991..2017
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      2456..2643
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3475..4637)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5151..5252)
                     /label="TRP1 promoter"
     promoter        5358..5462
                     /label="AmpR promoter"
     CDS             5463..6320
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6494..7082
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgaactat ctattcgatg atgaagatac
      901 cccaccaaac ccaaaaaaag agatcgaatt ggagaatttg tattttcagg gcgaattcca
      961 gctgaccacc atggcaatga aagtgaatat ggagtacaca aaggaaaaga aagttggtga
     1021 gggtacttat gcggttgttt acttgggttg tcaacactct actggaagaa agattgctat
     1081 taaggagatc aaaacatccg aatttaaaga tggtttagat atgtcagcta tccgtgaagt
     1141 taagtacctc caagaaatgc agcatccgaa cgtcatagaa ctaatagaca tatttatggc
     1201 ttatgataat ttaaatctcg ttctggagtt cctaccaact gatctagagg tggtaataaa
     1261 agacaaatca atactgttta caccagcaga tattaaggca tggatgctta tgactttgag
     1321 gggcgtgtat cattgccaca gaaatttcat tttgcacagg gatctgaaac caaacaattt
     1381 attattttca cctgatggcc agataaaagt agcagatttc ggtctagcaa gggcgatacc
     1441 ggccccacat gagatactga caagtaacgt cgtaacaaga tggtatagag cgccagaatt
     1501 gttgtttgga gctaaacatt acacatcggc tattgatatc tggtcagtag gcgttatatt
     1561 cgcggaatta atgctaagga taccttattt accaggacag aatgatgtcg atcaaatgga
     1621 agtaacgttc agggccttag ggacacctac agatagagat tggcccgaag tttcttcctt
     1681 tatgacgtat aacaagttac aaatatatcc gcccccttca agagatgaat tgaggaaaag
     1741 gttcattgct gctagcgaat acgccttaga ttttatgtgt ggaatgctaa cgatgaaccc
     1801 acaaaagagg tggaccgctg ttcagtgttt agaaagtgat tatttcaaag aattaccacc
     1861 accaagtgac ccgtcttcaa taaaaatacg taacgtcatg gcaattcccg gtggcggccg
     1921 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     1981 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcggcc gctctagcta
     2041 gaactagtgg atcccccgat accgtcgacc tgcagagatc tatgaatcgt agatactgaa
     2101 aaaccccgca agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata
     2161 catcgttttg ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa
     2221 cctctgatct atagaatttt ttaaatgact agaattaatg cccatctttt ttttggacct
     2281 aaattcttca tgaaaatata ttacgagggc ttattcagaa gctttggact tcttcgccag
     2341 aggtttggtc aagtctccaa tcaaggttgt cggcttgtct accttgccag aaatttacga
     2401 aaagatggaa aagggtcaaa tcgttggtag atacgttgtt gacacttcta aataagcgaa
     2461 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     2521 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     2581 gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac
     2641 cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc acccaattgt agatatgcta
     2701 actccagcaa tgagttgatg aatctcggtg tgtattttat gtcctcagag gacaacacct
     2761 gttgtaatcg ttcttccaca cggatcctgg cgtaatagcg aagaggcccg caccgatcgc
     2821 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     2881 acgcatctgt gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc
     2941 ttgaatttat tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag
     3001 tggttgacat gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat
     3061 tttacaatca aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc
     3121 ttataaaaaa gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt
     3181 atctatgctg tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc
     3241 cgggtaaata catataatac acaaatctgg cttaataaag tctataatat atctcataaa
     3301 gaagtgctaa attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat
     3361 atcgactttg taaaagttca ctttagcata catatattac acgagccaga aattgtaact
     3421 tttgcctaaa atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat
     3481 caactttttt aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata
     3541 atttatttat tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt
     3601 tgtccttgga aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta
     3661 aactctttac agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg
     3721 ttttatcgtc acagttttac agtaaataag tatcacctct tagagttcga tgataagctg
     3781 tcaaacatga gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt
     3841 ggaccgaacg tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc
     3901 aacgctaatt atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg
     3961 acgacttgag gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt
     4021 ttgatattac tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat
     4081 aagtgtgata aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat
     4141 acggagagag gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga
     4201 ccgagaaaag gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt
     4261 caacaagttt gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt
     4321 tcctgtacaa tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt
     4381 acagatttta tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac
     4441 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa
     4501 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca
     4561 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct
     4621 aacataaaat gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc
     4681 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga
     4741 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta
     4801 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg
     4861 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga
     4921 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt
     4981 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata
     5041 cccagcaagt cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg
     5101 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct
     5161 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct
     5221 tggccctctc cttttctttt ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga
     5281 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5341 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5521 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5581 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5641 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5701 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5761 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5821 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5881 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5941 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6001 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6061 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6121 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6181 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6241 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6301 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6601 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7201 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7261 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagga
     7321 tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag
     7381 acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt
     7441 tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc
     7501 cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt
     7561 gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga
     7621 atgccggttg gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc
     7681 gaaagaacct gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga
     7741 gacgcgagtt tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg
     7801 cataaccgct agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag
     7861 acaggtacat acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg
     7921 tgtgcacttt attatgttac aatatggaag ggaactttac acttctccta tgcacatata
     7981 ttaattaaag tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga
     8041 tttttttcta aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat
     8101 ggacttcctc ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg
     8161 gtctccctaa catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga
     8221 cataatgggc taaacaagac tacaccaatt acactgcctc attgatg
//
