LOCUS       V015307                 7813 bp    DNA     circular SYN 12-JUN-2019
DEFINITION  Exported.
ACCESSION   V015307
VERSION     V015307
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7813)
  AUTHORS   Wang R, Ghumra A, Fairclough L, Alcocer M.
  TITLE     Nut lipids are involved in the intrinsic allergenicity of nut
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7813)
  AUTHORS   Wang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2019) School of Biosciences, University of
            Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE   3  (bases 1 to 7813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2019) School of Biosciences, University of Nottingham, Babs
            Building, Sutton Bonington LE12 5RD, UK"
FEATURES             Location/Qualifiers
     source          1..7813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             1363..1374
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             complement(1470..1820)
                     /gene="TRGV4"
                     /label="T cell receptor gamma variable 4"
                     /note="T cell receptor gamma variable 4 from Homo sapiens.
                     Accession#: A0A0C4DH28"
     promoter        complement(1975..2178)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2179..2482)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3327..3785
                     /gene="TRDC"
                     /label="T cell receptor delta constant"
                     /note="T cell receptor delta constant from Homo sapiens.
                     Accession#: B7Z8K6"
     polyA_signal    3826..4050
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4096..4524
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4538..4868
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4916..4963
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4982..5353
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5486..5619
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5656..5672)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5680..5696)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5704..5734)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5749..5770)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6058..6646)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6820..7677)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7678..7782)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct
      781 taataaaccc aatgcctttt tcttattaat gcccaacttt gtgacccaag aaaggttggg
      841 gttagatttt cttttccatt tgcaggaaga aaacccgttt cttttaaggc agacagcagg
      901 tggatatggc caaaataggc ccccctcttg aggagccgga ggaggtacgt gtaatatgca
      961 gaggtgtttg tgagctgcag cattagtgta tcatttgcat cttttgacca attgtctttg
     1021 gggtccattg tgatgacatc atttgcatct tttgaataac tgtctttggg gtccactgtg
     1081 gtgacatctg tctttattgg aggaaagata atttcttgat caattccgtt tttattattc
     1141 tcatgtctga cgatacatct gtgttctttg tccagtgact cttctggcac cgttaaccag
     1201 ctaaatttca tgtatgtgtc gttagtcttc atggtgttcc cctcctggga gcccagaatc
     1261 gtgttgctct tcttttcttg ccaatgtatc ttaataatat ctgggaaaaa tttctcaaga
     1321 agacaaaggt atgttccagc cttctggagt tttgtttcag caattgaagg aagaaaaata
     1381 gtgggcttgg gggaaacatc tgcatcaagt tgtttatctg tgacaacaag tgttgttcca
     1441 ctgccaaaga gtttcttata atacttctca tcccaggtgg cacagtaata gactccagag
     1501 tcattttcaa taagatttcg cagtatcatt ctcaagttct tccttgtgct tccataagta
     1561 tcatacttcc ctgggctgat tcctgattcc aacacaacgc tggaggtgta ggagtcatag
     1621 tacagaagac gctgtggggc cttcccctcc tggtgtagat accagtggat gtagccggta
     1681 cttccttcag caagatcaca agtgatttca gcagatgacc cagtctgcct gatgactgac
     1741 ttcgttctcc cttccaagtt ggaagatttc tgactggcag gagacaggaa agctagaagc
     1801 accgctagag cccactgcat gccttcctct ctgaggcctt ggaaggaaag ggaccagatg
     1861 aagaggagca gaagatcctc ttaaaactca ggccccactc aacaaggaag tgatccctag
     1921 cacagaagac agtaggaggg aggatcccta gcggatctga cggttcacta aaccagctct
     1981 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     2041 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     2101 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     2161 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     2221 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     2281 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     2341 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     2401 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     2461 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     2521 aattgattac tattaataac tactcgagta tgtcgaggtg gcgtgtacgg tgggaggcct
     2581 atataagcag agctcgttta gtgaaccgtc agatcgcctg gaggtaccac cggtcatatg
     2641 ccgcgggagc ccagatcctg tgtgtgtgtg tgtgtgcttg tgcacatctg ttctctgatt
     2701 ggctgactgt acttcctgag agtccctgtt taatgttaca agaaacagct gatgcagagg
     2761 atctcctccc tcatccatct ctctctcttc tgggcaggta aggcagaccc cagaacttgg
     2821 gccaagcaag actcagcagc aaagaaacat caaggctcac aggacctcgt actcatggac
     2881 tgtgctcctt actcaatgac ctcagccccc agccctcact gagcccctct cttgtgtctt
     2941 ttctctcagg agtcatgtca gccattgagt tggtgcctga acaccaaaca gtgcctgtgt
     3001 caataggggt ccctgccacc ctcaggtgct ccatgaaagg agaagcgatc ggtaactatt
     3061 atatcaactg gtacaggaag acccaaggta acacaatgac tttcatatac cgagaaaagg
     3121 acatctatgg ccctggtttc aaagacaatt tccaaggtga cattgatatt gcaaagaacc
     3181 tggctgtact taagatactt gcaccatcag agagagatga agggtcttac tactgtgcct
     3241 gtgaccccgt accgggggat acaacacaca caagcgacga taaactcatc tttggaaaag
     3301 gaacccgtgt gactgtggaa ccaagaagtc agcctcatac caaaccatcc gtttttgtca
     3361 tgaaaaatgg aacaaatgtc gcttgtctgg tgaaggaatt ttaccccaag gatataagaa
     3421 taaatctcgt gtcatccaag aagataacag agtttgatcc tgctattgtc atctctcccg
     3481 gtgggaagta caatgctgtc aagcttggta aatatgaaga ttcaaattca gtgacatgtt
     3541 cagttcaaca cgacaataaa actgtgcact ccactgactt tgaagtgaag acagattcta
     3601 cagatcacgt aaaaccaaag gaaactgaaa acacaaagca accttcaaag agctgccata
     3661 aacccaaagc catagttcat accgagaagg tgaacatgat gtccctcaca gtgcttgggc
     3721 tacgaatgct gtttgcaaag actgttgccg tcaattttct cttgactgcc aagttatttt
     3781 tcttgtaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     3841 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3901 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3961 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4021 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4081 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4141 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4201 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt
     4261 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4321 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4381 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4441 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga
     4501 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4561 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4621 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4681 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4741 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4801 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4861 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4921 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     4981 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     5041 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     5101 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     5161 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     5221 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     5281 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     5341 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     5401 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5461 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5581 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5641 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5701 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5761 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5821 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5881 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5941 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6001 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6061 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6121 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6181 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6241 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6301 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6361 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6421 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6481 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6541 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6601 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6661 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6721 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6781 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6901 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7141 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7441 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7681 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7741 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7801 gccacctgac gtc
//
