LOCUS       62056_16865        7751 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1832..3481)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(3506..3709)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(3710..4013)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(4135..4153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4165..4183)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4203..4219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4227..4243)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4251..4281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4296..4317)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4605..5193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5367..6224)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6225..6329)
                     /label=AmpR promoter
     polyA_signal    complement(6417..6498)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6731..7327)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(7424..7740)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgta acctcagcaa ctaaaaataa aaacttcagt atataacttg
      541 ggaacaaaaa agtagtaaag ggaagcaatt caagatctgt tattggtatt tgaatacatt
      601 tattgtgaca gaatgctgtt ataaatattc ataagacaaa ggccatcttt ttatctagga
      661 attgtcaaag agaagattcc aaattggaag gatacatctt ttgtaaaatc tgccaccaat
      721 tcctgctttg agaataagca cctattgtaa aatttctaac attataaatg gtcacagcac
      781 atgccacttg atgcaatcca aactttgaaa tgtttgactt ctcagtgggc tgtccctctc
      841 cactgcaacc ccccttcctc cagcctcctg aaacatcgca ctagcctttg gtaagcaatt
      901 ccatatagat acgctggggg agcgtggagt atgaagcctg acatagcatc aggtacatca
      961 ggtacattta tttctaagtc taatagagaa cagtttttac tgcttaatag taagaagcac
     1021 tgagagtgat tttatcgagc attcttaact ctttcaactc cacctagata gactaggatc
     1081 tacagatgat gcagaaaaga ggttagggga gtactttcca atagtttatt gtattttctt
     1141 aaatatcctt tctggaattt tcagaaacaa aacataaaaa aattatatac tttattacaa
     1201 atggtaaact cagagtgctc caaatctctt atttacaaac aacactgggc aggataccca
     1261 aacaaacaaa caagaaataa cttacaaagg catgaagctg tttattgaca gtaatcagct
     1321 ttaatcaaat taaaaaatat atatatgtac atacacagtt aacgaaagca ggccagaaag
     1381 agttcatctg taggctcagc ctcgctctca catgttctta aggagtacta cagaagcaat
     1441 ctacagtctc tattgcagtt tgtaaccccc tccccctccc ccctttaata ctgaatgaga
     1501 tcgaatgtta ggtccatgca gttcttggtc aatgttaacg aaagtccaac gttccgttcg
     1561 cgcggggaca gcccgtccgc aaagcggggc agcccgcagg cggccgctcc ctggctccac
     1621 gccaagggag ggcgcgacca agtccttccc actcgtgcac actgggggcg ccgtcagacc
     1681 gaacccagtc caacttggat acccttggct ttagttctcg gacacttctt ttttctctcc
     1741 gtcgcaactt gtcaagttct caagtctgtc tctctgtgtt attttttttc ttcgttgccc
     1801 ctcagccccc gacggtctct cttcactcga gcaatttgga ctttccgccc ttcttggcct
     1861 ttatgaggat ctctctgatt tttcttgcgt cgagttttcc ggtaagacct ttcggtactt
     1921 cgtccacaaa cacaactcct ccgcgcaact ttttcgcggt tgttacttga ctggcgacgt
     1981 aatccacgat ctctttttcc gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg
     2041 gaagttcacc ggcgtcatcg tcgggaagac ctgccacgcc cgcgtcgaag atgttggggt
     2101 gttgtaacaa tatcgattcc aattcagcgg gggccacctg atatcctttg tatttaatta
     2161 aagacttcaa gcggtcaact atgaagaagt gttcgtcttc gtcccagtaa gctatgtctc
     2221 cagaatgtag ccatccatcc ttgtcaatca aggcgttggt cgcttccgga ttgtttacat
     2281 aaccggacat aatcataggt cctctgacac ataattcgcc tctctgatta acgcccagcg
     2341 ttttcccggt atccagatcc acaaccttcg cttcaaaaaa tggaacaact ttaccgaccg
     2401 cgcccggttt atcatccccc tcgggtgtaa tcagaatagc tgatgtagtc tcagtgagcc
     2461 catatccttg tcgtatccct ggaagatgga agcgttttgc aaccgcttcc ccgacttctt
     2521 tcgaaagagg tgcgccccca gaagcaattt cgtgtaaatt agataaatcg tatttgtcaa
     2581 tcagagtgct tttggcgaag aatgaaaata gggttggtac tagcaacgca ctttgaattt
     2641 tgtaatcctg aagggatcgt aaaaacagct cttcttcaaa tctatacatt aagacgactc
     2701 gaaatccaca tatcaaatat ccgagtgtag taaacattcc aaaaccgtga tggaatggga
     2761 caacacttaa aatcgcagta tccggaacga tttgattgcc aaaaatagga tctctggcat
     2821 gcgagaatct gacgcaggca gttctatgcg gaagggccac acccttaggt aacccagtag
     2881 atccagagga attcattatc agtgcaattg ttttgtcacg atcaaaggac tctggtacaa
     2941 aatcgtattc attaaaaccg ggaggtagat gagatgtgac gaacgtgtac atcgactgaa
     3001 atccctggta atccgtttta gaatccatga taataatttt ctggattatt ggtaattttt
     3061 tttgcacgtt caaaattttt tgcaacccct ttttggaaac aaacactacg gtaggctgcg
     3121 aaatgttcat actgttgagc aattcacgtt cattataaat gtcgttcgcg ggcgcaactg
     3181 caactccgat aaataacgcg cccaacaccg gcataaagaa ttgaagagag ttttcactgc
     3241 atacgacgat tctgtgattt gtattcagcc catatcgttt catagcttct gccaaccgaa
     3301 cggacatttc gaagtattcc gcgtacgtga tgttcacctc gatatgtgca tctgtaaaag
     3361 caattgttcc aggaaccagg gcgtatctct tcatagcctt atgcagttgc tctccagcgg
     3421 ttccatcctc tagaggatag aatggcgccg ggcctttctt tatgtttttg gcgtcttcca
     3481 tggtggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
     3541 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     3601 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     3661 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     3721 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     3781 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     3841 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     3901 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     3961 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     4021 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg
     4081 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata
     4141 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc
     4201 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5041 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5101 ggtagctctt gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     6361 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag
     6421 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     6481 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     6541 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     6601 gatcctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt
     6661 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg
     6721 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     6781 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     6841 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     6901 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     6961 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     7021 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     7081 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     7141 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     7201 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     7261 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     7321 cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg
     7381 gcaccggtcg tggcggccga cctgcaggca tgcaagctag ctttttgcaa aagcctaggc
     7441 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     7501 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     7561 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     7621 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
     7681 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     7741 aatctttcgt c
//
