LOCUS       62056_16855        6613 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(694..2343)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(2368..2571)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2572..2875)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(2997..3015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3027..3045)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3065..3081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3089..3105)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3113..3143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3158..3179)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4229..5086)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5087..5191)
                     /label=AmpR promoter
     polyA_signal    complement(5279..5360)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5593..6189)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(6286..6602)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgtt cgcattcaac ttaaatgctt ttattgacaa tgtcttggaa
      541 caataagcaa acaatgctta aatttttcat tcaaattcac tttccacatg tcaaaagacc
      601 tcaaggtaga aaaaaataaa ataaaaatat aaatatctga gaatccatct taataaataa
      661 attaaaaaca caataaaacg tttttcactc gagcaatttg gactttccgc ccttcttggc
      721 ctttatgagg atctctctga tttttcttgc gtcgagtttt ccggtaagac ctttcggtac
      781 ttcgtccaca aacacaactc ctccgcgcaa ctttttcgcg gttgttactt gactggcgac
      841 gtaatccacg atctcttttt ccgtcatcgt ctttccgtgc tccaaaacaa caacggcggc
      901 gggaagttca ccggcgtcat cgtcgggaag acctgccacg cccgcgtcga agatgttggg
      961 gtgttgtaac aatatcgatt ccaattcagc gggggccacc tgatatcctt tgtatttaat
     1021 taaagacttc aagcggtcaa ctatgaagaa gtgttcgtct tcgtcccagt aagctatgtc
     1081 tccagaatgt agccatccat ccttgtcaat caaggcgttg gtcgcttccg gattgtttac
     1141 ataaccggac ataatcatag gtcctctgac acataattcg cctctctgat taacgcccag
     1201 cgttttcccg gtatccagat ccacaacctt cgcttcaaaa aatggaacaa ctttaccgac
     1261 cgcgcccggt ttatcatccc cctcgggtgt aatcagaata gctgatgtag tctcagtgag
     1321 cccatatcct tgtcgtatcc ctggaagatg gaagcgtttt gcaaccgctt ccccgacttc
     1381 tttcgaaaga ggtgcgcccc cagaagcaat ttcgtgtaaa ttagataaat cgtatttgtc
     1441 aatcagagtg cttttggcga agaatgaaaa tagggttggt actagcaacg cactttgaat
     1501 tttgtaatcc tgaagggatc gtaaaaacag ctcttcttca aatctataca ttaagacgac
     1561 tcgaaatcca catatcaaat atccgagtgt agtaaacatt ccaaaaccgt gatggaatgg
     1621 gacaacactt aaaatcgcag tatccggaac gatttgattg ccaaaaatag gatctctggc
     1681 atgcgagaat ctgacgcagg cagttctatg cggaagggcc acacccttag gtaacccagt
     1741 agatccagag gaattcatta tcagtgcaat tgttttgtca cgatcaaagg actctggtac
     1801 aaaatcgtat tcattaaaac cgggaggtag atgagatgtg acgaacgtgt acatcgactg
     1861 aaatccctgg taatccgttt tagaatccat gataataatt ttctggatta ttggtaattt
     1921 tttttgcacg ttcaaaattt tttgcaaccc ctttttggaa acaaacacta cggtaggctg
     1981 cgaaatgttc atactgttga gcaattcacg ttcattataa atgtcgttcg cgggcgcaac
     2041 tgcaactccg ataaataacg cgcccaacac cggcataaag aattgaagag agttttcact
     2101 gcatacgacg attctgtgat ttgtattcag cccatatcgt ttcatagctt ctgccaaccg
     2161 aacggacatt tcgaagtatt ccgcgtacgt gatgttcacc tcgatatgtg catctgtaaa
     2221 agcaattgtt ccaggaacca gggcgtatct cttcatagcc ttatgcagtt gctctccagc
     2281 ggttccatcc tctagaggat agaatggcgc cgggcctttc tttatgtttt tggcgtcttc
     2341 catggtggat ccacggttca ctaaaccagc tctgcttata tagacctccc accgtacacg
     2401 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     2461 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     2521 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     2581 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     2641 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     2701 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2761 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2821 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2881 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactaagatc
     2941 tggtaccttg aattcatgct tctcctccct ttagtgaggg taattctctc tctctcccta
     3001 tagtgagtcg tattaattcc ttctcttcta tagtgtcacc taaatcgttg caattcgtaa
     3061 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3301 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3361 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3421 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3481 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3541 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3601 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3661 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3721 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3781 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3841 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3901 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3961 ttggtagctc ttgatccggc aaaaaaacca ccgctggtag cggtggtttt tttgtttgca
     4021 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4081 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4141 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4201 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4261 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4321 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4381 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4441 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4501 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4561 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4621 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4681 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4741 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4801 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4861 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4921 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4981 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5041 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5101 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5161 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5221 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgagattgc
     5281 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5341 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5401 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     5461 atgatcctct agagtcggtg ggcctcgggg gcgggtgcgg ggtcggcggg gccgccccgg
     5521 gtggcttcgg tcggagccat ggggtcgtgc gctcctttcg gtcgggcgct gcgggtcgtg
     5581 gggcgggcgt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac
     5641 ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc
     5701 ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac
     5761 gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa
     5821 ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc
     5881 cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc
     5941 ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac
     6001 cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc
     6061 gatgtggcgg tccgggtcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc
     6121 gagggtgcgt acggcccggg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta
     6181 ctcggtcatg gaaggtcgtc tccttgtgag gggtcagggg cgtgggtcag gggatggtgg
     6241 cggcaccggt cgtggcggcc gacctgcagg catgcaagct agctttttgc aaaagcctag
     6301 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     6361 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     6421 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
     6481 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
     6541 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6601 ccaatctttc gtc
//
