LOCUS       62056_16850        6592 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(673..2322)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIVPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     promoter        complement(2347..2550)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2551..2854)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(2976..2994)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3006..3024)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3044..3060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3068..3084)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3092..3122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3137..3158)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3446..4034)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4208..5065)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5066..5170)
                     /label=AmpR promoter
     polyA_signal    complement(5258..5339)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5572..6168)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(6265..6581)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa
      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttaac tgtttaaacc
      481 tatgatatca cgcacgcgtt cgcattcaac tgcttttatt gacaatgtct tggaacaata
      541 agcaaacaat gctttttcat tcaaattcac tttccacatg tcaaaagacc tcaaggtaga
      601 aaaaaataaa ataaaaataa tctgagaatc catcttaata aaaaaaacac aataaaacgt
      661 ttttcactcg agcaatttgg actttccgcc cttcttggcc tttatgagga tctctctgat
      721 ttttcttgcg tcgagttttc cggtaagacc tttcggtact tcgtccacaa acacaactcc
      781 tccgcgcaac tttttcgcgg ttgttacttg actggcgacg taatccacga tctctttttc
      841 cgtcatcgtc tttccgtgct ccaaaacaac aacggcggcg ggaagttcac cggcgtcatc
      901 gtcgggaaga cctgccacgc ccgcgtcgaa gatgttgggg tgttgtaaca atatcgattc
      961 caattcagcg ggggccacct gatatccttt gtatttaatt aaagacttca agcggtcaac
     1021 tatgaagaag tgttcgtctt cgtcccagta agctatgtct ccagaatgta gccatccatc
     1081 cttgtcaatc aaggcgttgg tcgcttccgg attgtttaca taaccggaca taatcatagg
     1141 tcctctgaca cataattcgc ctctctgatt aacgcccagc gttttcccgg tatccagatc
     1201 cacaaccttc gcttcaaaaa atggaacaac tttaccgacc gcgcccggtt tatcatcccc
     1261 ctcgggtgta atcagaatag ctgatgtagt ctcagtgagc ccatatcctt gtcgtatccc
     1321 tggaagatgg aagcgttttg caaccgcttc cccgacttct ttcgaaagag gtgcgccccc
     1381 agaagcaatt tcgtgtaaat tagataaatc gtatttgtca atcagagtgc ttttggcgaa
     1441 gaatgaaaat agggttggta ctagcaacgc actttgaatt ttgtaatcct gaagggatcg
     1501 taaaaacagc tcttcttcaa atctatacat taagacgact cgaaatccac atatcaaata
     1561 tccgagtgta gtaaacattc caaaaccgtg atggaatggg acaacactta aaatcgcagt
     1621 atccggaacg atttgattgc caaaaatagg atctctggca tgcgagaatc tgacgcaggc
     1681 agttctatgc ggaagggcca cacccttagg taacccagta gatccagagg aattcattat
     1741 cagtgcaatt gttttgtcac gatcaaagga ctctggtaca aaatcgtatt cattaaaacc
     1801 gggaggtaga tgagatgtga cgaacgtgta catcgactga aatccctggt aatccgtttt
     1861 agaatccatg ataataattt tctggattat tggtaatttt ttttgcacgt tcaaaatttt
     1921 ttgcaacccc tttttggaaa caaacactac ggtaggctgc gaaatgttca tactgttgag
     1981 caattcacgt tcattataaa tgtcgttcgc gggcgcaact gcaactccga taaataacgc
     2041 gcccaacacc ggcataaaga attgaagaga gttttcactg catacgacga ttctgtgatt
     2101 tgtattcagc ccatatcgtt tcatagcttc tgccaaccga acggacattt cgaagtattc
     2161 cgcgtacgtg atgttcacct cgatatgtgc atctgtaaaa gcaattgttc caggaaccag
     2221 ggcgtatctc ttcatagcct tatgcagttg ctctccagcg gttccatcct ctagaggata
     2281 gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggatc cacggttcac
     2341 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     2401 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     2461 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     2521 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     2581 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     2641 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     2701 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     2761 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     2821 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     2881 taatgacccc gtaattgatt actattaata actaagatct ggtaccttga attcatgctt
     2941 ctcctccctt tagtgagggt aattctctct ctctccctat agtgagtcgt attaattcct
     3001 tctcttctat agtgtcacct aaatcgttgc aattcgtaat catgtcatag ctgtttcctg
     3061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     3121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     3181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     3241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3961 aaaaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5221 tgacattaac ctataaaaat aggcgtatca cgagattgca gtgaaaaaaa tgctttattt
     5281 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     5341 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     5401 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gagtcggtgg
     5461 gcctcggggg cgggtgcggg gtcggcgggg ccgccccggg tggcttcggt cggagccatg
     5521 gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg
     5581 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     5641 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     5701 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     5761 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     5821 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     5881 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     5941 ccagaccgcc accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     6001 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccgggtcgac
     6061 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggcccgggg
     6121 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg aaggtcgtct
     6181 ccttgtgagg ggtcaggggc gtgggtcagg ggatggtggc ggcaccggtc gtggcggccg
     6241 acctgcaggc atgcaagcta gctttttgca aaagcctagg cctccaaaaa agcctcctca
     6301 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     6361 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     6421 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     6481 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     6541 catacttctg cctgctgggg agcctgggga ctttccacac caatctttcg tc
//
