LOCUS       62056_16805        7278 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             180..578
                     /codon_start=1
                     /label=smURFP
                     /note="dimeric small ultra-red fluorescent protein derived
                     from a cyanobacterial phycobiliprotein (Rodriguez et al., 
                     2016)"
                     /translation="MAKTSEQRVNIATLLTENKKKIVDKASQDLWRRHPDLIAPGGIAF
                     SQRDRALCLRDYGWFLHLITFCLLAGDKGPIESIGLISIREMYNSLGVPVPAMMESIRC
                     LKEASLSLLDEEDANETAPYFDYIIKAMS"
     misc_feature    579..644
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1749..2291
                     /codon_start=1
                     /label=p65
                     /note="C-terminal portion of the p65 subunit of mouse
                     NF-kappa-B"
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     LTR             2396..2910
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(3072..3088)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3096..3126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3141..3162)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3450..4038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4212..5069)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5070..5174)
                     /label=AmpR promoter
     LTR             6002..6518
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"
     misc_feature    6582..6923
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             join(6990..7278,1..128)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
       61 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
      121 taggcgccgg aattagatct ctcgaggtta acgaattccg cccccccccc ctagccacca
      181 tggctaagac ttccgaacag agggtgaaca ttgctacact gctgacagaa aataagaaga
      241 aaatcgtgga taaggcttcc caggatctgt ggcggagaca cccagacctg atcgcaccag
      301 gaggaattgc tttctctcag agggaccgcg ctctgtgcct gcgagattac ggctggttcc
      361 tgcatctgat caccttttgt ctgctggccg gagataaggg ccccatcgag tctattgggc
      421 tgatcagtat tcgagaaatg tataactcac tgggagtgcc cgtccctgca atgatggaga
      481 gcattagatg cctgaaagaa gccagcctgt ccctgctgga cgaagaggac gccaacgaga
      541 ccgcacccta ctttgattac attattaagg ctatgagctc cggactcaga tctcgagctc
      601 aagcttcgaa ctctgcagtg gacggtaccg cgggcccggg atccatggac gatctgtttc
      661 ccctcatctt tccctcagag ccagcccagg cttctgggcc ttatgtggag atcatcgaac
      721 agccgaagca acggggcatg cgattccgct ataaatgcga ggggcgctca gcgggcagta
      781 ttcctggcga gagaagcaca gataccacca agacacaccc caccatcaag atcaatggct
      841 acacaggacc aggaacagtt cgaatctccc tggtcaccaa ggatccacct caccggcctc
      901 atccacatga acttgtgggg aaggactgcc gggatggcta ctatgaggct gacctctgcc
      961 cagaccgcag tatccatagc ttccagaacc tggggatcca gtgtgtgaag aagcgagacc
     1021 tggagcaagc cattagccag cgaatccaga ccaacaataa cccctttcac gttcctatag
     1081 aggagcagcg cggggactat gacttgaatg cagtgcgcct ctgcttccag gtgacagtgc
     1141 gggacccagc aggcaggccc ctcctcctga cccctgtcct ctcacatccg atttttgata
     1201 accgggcccc caacactgcc gagctcaaga tctgccgagt aaaccggaac tctgggagct
     1261 gcctcggtgg ggatgagatc ttcttgctgt gcgacaaggt gcagaaagaa gacattgagg
     1321 tgtatttcac gggaccaggc tgggaggcac gaggctcctt ttctcaagct gatgtgcatc
     1381 ggcaagtggc cattgtgttc cggactcctc cgtacgccga ccccagcctc caggctcctg
     1441 ttcgagtctc catgcagcta cggcggcctt ctgatcgcga gctcagtgag cccatggagt
     1501 tccagtactt gccagacaca gatgatcgcc accggattga agagaagcgc aaaaggacct
     1561 atgagacctt caagagtatc atgaagaaga gtcctttcaa tggaccaact gaaccccggc
     1621 ctccaacccg gcgtattgct gtgcctaccc gaaactcaac ttctgtcccc aagccagccc
     1681 cgcagcccta caccttccca gcatccctca gcaccatcaa ctttgatgag ttttccccca
     1741 tgctgttacc atcagggcag atctcaaacc aggccctggc cttggcaccg tcctctgccc
     1801 cagtccttgc ccagaccatg gtcccttcct cagccatggt acctctggct cagcccccag
     1861 ctcctgcccc agttctaacc ccgggtcctc cccagtccct gtctgcacct gttccaaaga
     1921 gcacccaggc tggggaaggc acgctgtcgg aagccctgct gcacctgcag tttgatgctg
     1981 atgaagactt gggggccttg cttggcaaca gcacagaccc aggagtgttc acagacctgg
     2041 catctgtgga caactcagag tttcagcagc tcctgaacca gggtgtgtcc atgtctcact
     2101 ccacagctga gcccatgctg atggagtacc ctgaagctat aactcgcctg gtgacagggt
     2161 cccagaggcc ccctgaccca gctcccacac ccctggggac ctcggggctt cccaatggtc
     2221 tctcaggaga tgaagacttc tcctccattg cggacatgga cttctctgct cttttgagtc
     2281 agatcagctc ctaatgaatt aattaagaat tatcaagctt atcgataccg tcgacctgca
     2341 gccaagctta tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     2401 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     2461 aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat
     2521 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     2581 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
     2641 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     2701 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     2761 ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat ccaataaacc
     2821 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     2881 attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt tggagaacaa
     2941 cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca ctgttttgaa
     3001 tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaaa gagctgggga
     3061 gaattgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3241 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     5221 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     5281 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5341 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5401 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     5461 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     5521 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     5581 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     5641 cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     5701 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     5761 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     5821 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta
     5881 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     5941 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     6001 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     6061 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     6121 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6181 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6241 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6301 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6361 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtattccca
     6421 ataaagcctc ttgctgtttg catccgaatc gtggactcgc tgatccttgg gagggtctcc
     6481 tcagattgat tgactgccca cctcgggggt ctttcatttg gaggttccac cgagatttgg
     6541 agacccctgc ccagggacca ccgacccccc cgccgggagg taagctggcc agcggtcgtt
     6601 tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat ctaatgtttg cgcctgcgtc
     6661 tgtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttct
     6721 gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt ttttgtggcc
     6781 cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg gttctggtag
     6841 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttggaacc
     6901 gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt ctctgtctga
     6961 ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga
     7021 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     7081 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     7141 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     7201 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     7261 acccccctcc ctgggtca
//
