LOCUS       pMIB_V5-His_C.        3592 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            A or pMIB/V5-His B.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His C.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     sig_peptide     565..627
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    629..721
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             730..771
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             781..798
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    816..945
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1736..2027
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2053..2100
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2119..2514
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2637..3494
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg
       61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt
      121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
      181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
      241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
      301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
      361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
      421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
      481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
      541 catctgttcg aatttaaagc taccatgaaa ttcttagtca acgttgccct tgtttttatg
      601 gtcgtataca tttcttacat ctatgccggc atgctaagct tggtaccgag ctcggatcca
      661 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgaggtcac
      721 ccattcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
      781 catcatcacc atcaccattg agtttatctg actaaatctt agtttgtatt gtcatgtttt
      841 aatacaatat gttatgttta aatatgtttt taataaattt tataaaataa tttcaacttt
      901 tattgtaaca acattgtcca tttacacact cctttcaagc gcgtgggatc gatgctcact
      961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcgaa
     1741 acacgcacgg cgcgcgcacg cagcttagca caaacgcgtc gttgcacgcg cccaccgcta
     1801 accgcaggcc aatcggtcgg ccggcctcat atccgctcac cagccgcgtc ctatcgggcg
     1861 cggcttccgc gcccattttg aataaataaa cgataacgcc gttggtggcg tgaggcatgt
     1921 aaaaggttac atcattatct tgttcgccat ccggttggta taaatagacg ttcatgttgg
     1981 tttttgtttc agttgcaagt tggctgcggc gcgcgcagca cctttgccgg gatctgccgg
     2041 gctgcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     2101 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     2161 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     2221 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     2281 ccttgcgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     2341 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     2401 caggttcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     2461 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     2521 cttcgtggcc gaggagcagg actgacacgt cccgggagat ctgcatgtct actaaactca
     2581 caaattagag cttcaattta attatatcag ttattaccca ttgaaaaagg aagagtatga
     2641 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2701 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2761 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2821 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2881 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2941 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3001 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3061 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3121 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3181 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3241 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3301 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3361 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3421 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3481 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3541 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ct
//
