LOCUS       pMIB_V5-His_B.        3600 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector for expression of secreted, C-terminally 
            V5-6xHis-tagged proteins. For other reading frames, use pMIB/V5-His 
            A or pMIB/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMIB V5-His B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..548
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     sig_peptide     565..627
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    629..731
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             738..779
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             789..806
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    824..953
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1081..1669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1744..2035
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        2061..2108
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2127..2522
                     /label=BSD
                     /note="blasticidin S deaminase"
     CDS             2645..3502
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg
       61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt
      121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc
      181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac
      241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc
      301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt
      361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca
      421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc
      481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag
      541 catctgttcg aatttaaagc taccatgaaa ttcttagtca acgttgccct tgtttttatg
      601 gtcgtataca tttcttacat ctatgccggc atgctaagct tggtaccgag ctcggatcca
      661 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag
      721 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
      781 gtaccggtca tcatcaccat caccattgag tttatctgac taaatcttag tttgtattgt
      841 catgttttaa tacaatatgt tatgtttaaa tatgttttta ataaatttta taaaataatt
      901 tcaactttta ttgtaacaac attgtccatt tacacactcc tttcaagcgc gtgggatcga
      961 tgctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1501 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1741 catgcgaaac acgcacggcg cgcgcacgca gcttagcaca aacgcgtcgt tgcacgcgcc
     1801 caccgctaac cgcaggccaa tcggtcggcc ggcctcatat ccgctcacca gccgcgtcct
     1861 atcgggcgcg gcttccgcgc ccattttgaa taaataaacg ataacgccgt tggtggcgtg
     1921 aggcatgtaa aaggttacat cattatcttg ttcgccatcc ggttggtata aatagacgtt
     1981 catgttggtt tttgtttcag ttgcaagttg gctgcggcgc gcgcagcacc tttgccggga
     2041 tctgccgggc tgcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     2101 aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc
     2161 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     2221 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     2281 ctgggggacc ttgcgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     2341 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     2401 ggtgccgaca ggttcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     2461 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     2521 gctaagcact tcgtggccga ggagcaggac tgacacgtcc cgggagatct gcatgtctac
     2581 taaactcaca aattagagct tcaatttaat tatatcagtt attacccatt gaaaaaggaa
     2641 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2701 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2761 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2821 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2881 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2941 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3001 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3061 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3121 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3181 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3241 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3301 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3361 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3421 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3481 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3541 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
//
