LOCUS       Exported                3600 bp DNA     circular SYN 03-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMG36e
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3600)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3600)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             221..919
                     /codon_start=1
                     /label=repA
                     /note="repA"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDCYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             complement(1454..2188)
                     /codon_start=1
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase, 
                     also known as Erm methyltransferase, is an enzyme that 
                     confers resistance to erythromycin and other macrolide, 
                     lincosamide, and streptogramin B (MLSB) antibiotics. It 
                     works by methylating the adenine at position 2058 of the 
                     23S ribosomal RNA, which prevents erythromycin from binding
                     to the ribosome and inhibiting protein synthesis. "
                     /label=Emr
                     /note="Emr"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     promoter        2465..2643
                     /note="P32"
     repeat_region   3065..3195
                     /label=IRI repeat region
                     /note="A cluster of identical repeat sequences (IRI) that
                     likely functions in genome organization, structural 
                     maintenance, or regulatory processes through repeated DNA 
                     elements.; id: 4274850"
     CDS             complement(3204..3215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   3242..3264
                     /label=IRII Inverted Repeat Region
                     /note="A cluster of identical inverted repeat sequences
                     (IRII) that likely function in DNA replication initiation, 
                     recombination, or regulatory processes by forming stem-loop
                     structures.; id: 4274849"
     repeat_region   3348..3396
                     /label=IRIII Repeat Region
                     /note="A cluster of inverted repeat sequences (IRIII) that 
                     likely function in DNA replication initiation, 
                     recombination regulation, or structural organization of 
                     genetic elements.; id: 4274848"
     CDS             3349..3558
                     /codon_start=1
                     /label=repC
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIVVFMITKR"
ORIGIN
        1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
       61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggcgttag ctatagaaga
      121 atatgcaaga aaggaatcag aacaaaaaaa ataagcgaaa gctcgcgttt ttagaaggat
      181 acgagttttc gctacttgtt tttgataagg taattatatc atggctatta aaaatactaa
      241 agctagaaat tttggatttt tattatatcc tgactcaatt cctaatgatt ggaaagaaaa
      301 attagagagt ttgggcgtat ctatggctgt cagtccttta cacgatatgg acgaaaaaaa
      361 agataaagat acatggaata gtagtgatgt tatacgaaat ggaaagcact ataaaaaacc
      421 acactatcac gttatatata ttgcacgaaa tcctgtaaca atagaaagcg ttaggaacaa
      481 gattaagcga aaattgggga atagttcagt tgctcatgtt gagatacttg attatatcaa
      541 aggttcatat gaatatttga ctcatgaatc aaaggacgct attgctaaga ataaacatat
      601 atacgacaaa aaagatattt tgaacattaa tgattttgat attgactgct atataacact
      661 tgatgaaagc caaaaaagag aattgaagaa tttactttta gatatagtgg atgactataa
      721 tttggtaaat acaaaagatt taatggcttt tattcgcctt aggggagcgg agtttggaat
      781 tttaaatacg aatgatgtaa aagatattgt ttcaacaaac tctagcgcct ttagattatg
      841 gtttgagggc aattatcagt gtggatatag agcaagttat gcaaaggttc ttgatgctga
      901 aacgggggaa ataaaatgac aaacaaagaa aaagagttat ttgctgaaaa tgaggaatta
      961 aaaaaagaaa ttaaggactt aaaagagcgt attgaaagat acagagaaat ggaagttgaa
     1021 ttaagtacaa caatagattt attgagagga gggattattg aataaataaa agccccctga
     1081 cgaaagtcga agggggtttt tattttggtt tgatgttgcg attaatagca atacaattgc
     1141 aataaacaaa atgatcgacc ggacccctat ctagcgaact tttagaaaag atataaaaca
     1201 tcagagtatg gacagttgcg gatgtacttc agaaaagatt agatgtctaa aaagctagct
     1261 ttttagacat ctaaatctag gtactaaaac aattcatcca gtaaaatata atattttatt
     1321 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
     1381 ccgatcgacc cgattcacaa aaaataggca cacgaaaaac aagttaaggg atgcagttta
     1441 tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat aagaattgtt
     1501 caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg ttaaattgat
     1561 tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa taattatact
     1621 tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata agtgagctat
     1681 tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata gaaatatcaa
     1741 cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt ttagcaaacc
     1801 cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt ttgcgtatta
     1861 tatccgtact tatgttataa ggtatattac catatatttt ataggattgg tttttaggaa
     1921 atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca acaagtttat
     1981 tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa ttacacctct
     2041 ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata ttatcatgtt
     2101 catttaatct tatatttgtc attattttat ctatattatg ttttgaagta ataaagtttt
     2161 gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt atatgaattt
     2221 tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat gaactgtgct
     2281 gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta gtataattat
     2341 agcacggtcg atcttctata taaaagatat attatcttat cagtattgtc aatatattca
     2401 aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt taaatatggg
     2461 tcgatcgaat tcggtcctcg ggatatgata agattaatag ttttagctat taatcttttt
     2521 ttatttttat ttaagaatgg cttaataaag cggttacttt ggatttttgt gagcttggac
     2581 tagaaaaaaa cttcacaaaa tgctatacta ggtaggtaaa aaaatattcg gaggaatttt
     2641 gaaatggcaa tcgtttcagc agaaaaattc gtaattcgag ctcgcccggg gatcgatcct
     2701 ctagagtcga cctgcaggca tgcaagcttt gcaaagtctg aaaacgaagg tggcagctgc
     2761 cgttgaagcg gccaagacag ttggtaaagg cgacggtaca accggtacta gcgacaaagg
     2821 cggcggtcaa ggtaccccgg cgctacgata tttggagttg aggttcaaag tcaaatggta
     2881 ctgatgaccg gtaaaattta atattttgaa ccttgcttag gcagctgact tcacattgtt
     2941 gagatcagct gccttttgct tatagttcat tgagtagaaa cggttctgtt gcgaagtttg
     3001 aaaatcaaac aagctcgatt ttttattaaa acgtctcaaa atcgtttctg agacgtttta
     3061 gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg tagcgtaaaa
     3121 gcccttgagc gtagcgtggc tttgcaggaa gatgttgtct gttagattat gaaagccgat
     3181 gactgaatga aataataagc gcagcgccct tctatttcgg ttggaggagg ctcaagggag
     3241 tatgagggaa tgaaattccc tcatgggttt gattttaaaa attgcttgca attttgccga
     3301 gcggtagcgc tggaaaattt ttgaaaaaaa tttggaattt ggaaaaaaat ggggggaaag
     3361 gaagcgaatt ttgcttccgt actacgaccc cccattaagt gccgagtgcc aatttttgtg
     3421 ccaaaaacgc tctatcccaa ctggctcaag ggtttaaggg gtttttcaat cgccaacgaa
     3481 tcgccaacgt tttcgccaac gttttttata aatctatatt taagtagctt tattgttgtt
     3541 tttatgatta caaagcgata cactaacttt ataaaattat ttgattggag ttttttaaat
//
