LOCUS       40924_30745        9177 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF334, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Expression and Visualization of Red Fluorescent Protein (RFP) in 
            Neurospora crassa
  JOURNAL   Fungal Genet. Newsl. 52 (2006) In press
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2005) Institute of Molecular Biology, University 
            of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA
REFERENCE   3  (bases 1 to 9177)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9177)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Newsl. 52 (2006) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2005) Institute of Molecular Biology, University of Oregon, 
            355 Streisinger Hall, Eugene, OR 97403-1229, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9177
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             4985..5662
                     /codon_start=1
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     promoter        complement(6497..6515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(9176..9177,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac
     4021 taaagggaac aaaagctggg taccgggccc tagaaggagc agtccatctg cgtgaatcac
     4081 gagagaatca gctactttga atcgatggat gcagctacca gaagtcactc agttcgttca
     4141 aagccacatc actgggcact tccattggga caggcattga tcggacgaga ccgacttctg
     4201 gccgctttca acagccacat tatatccatg tcacggctac gcgtcggcct tcggtaacag
     4261 aaaagcacac agacagcgat tgtgacatgg attcgggcaa agcattggtg gtcgcaccag
     4321 gtcatcctga gtgtgcagtg gctgctattc agatttcatc taactgcggg agaggggttc
     4381 aaaggggcgt gacgtcacag acaacgggtg aaggacgaag attgcctcac ttctttgcta
     4441 gcaatttgcc tgcaaagaag gcgcacatga caagcaaaca actgggaaac cactattgaa
     4501 tacccacaat gcaaagctcg gaaggtacgt cttgattgca gtgtgtcgag tgtcaaaaaa
     4561 gaagcaagtg ttcatgcaag ccaaaattgg cacctcctcc acttctccga gtgccccacc
     4621 cgaacctcca ggcgagatgg ccggaacata ccatccgcgt tgggattatg acgtatctcc
     4681 ttcttcttca catgattcca tcccgttgtt gcttgtttgc gagctgtgac gggagatcgt
     4741 agatgccact tcgggccagg caggcagtgc aggcagccag gaacacaagc ttccaacttg
     4801 gtcatctcga ttgccgatta agggaaccaa atgcctatat aagactgtcc tcccacctcc
     4861 ccaataccat tcttttcttc ttccatcatc agccaacaaa gcaatcacat cttcactact
     4921 tcaaatcaac acaacactca aaccactttc acaacccctc acatcaacca aagaattcca
     4981 aaacatggtg gcctcctccg aggacgtcat caaagagttc atgcgcttca aggtgcgcat
     5041 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     5101 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     5161 ggacatcctg tccccccagt tccagtacgg ctccaaggcg tacgtgaagc accccgccga
     5221 catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     5281 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     5341 gatctacaag gtgaagttcc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     5401 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     5461 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     5521 gaccatctac atggccaaga agcccgtgca gctgcccggc tactactacg tggacaccaa
     5581 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     5641 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     5701 caccgcctcc tccgaggacg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     5761 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5821 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     5881 cctgtccccc cagttccagt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     5941 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6001 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     6061 caaggtgaag ttccgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     6121 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     6181 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     6241 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggaca ccaagctgga
     6301 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     6361 ccaccacctg ttcctgggta gtggaggtgg cgcgcctact agtgccggca cgcgtggccg
     6421 gccagctagt agatctgcgg ccgcggctcg agggtacctc tagagcggcc gccaccgcgg
     6481 tggagctcca attcgcccta tagtgagtcg tattaagggc gtggacggct aatggggtct
     6541 gaatgctaaa agacaccatt tcccacactc cctctgattt tttttatggt ttttttttat
     6601 cttcattttt cgccgtcttt tattcggttg tttgggaggt tcacatgcaa cgtgcaagac
     6661 tgcaagtttt ttgagatggg cgcttcatgc aatgcagcat cactgcaggg acattgtgac
     6721 atccacgttt cgatcgtctg tgcttccccc gttcatgtac gcatgatctg aaaaaaaaaa
     6781 aaaaaaaaaa ggttgatgta ataatgaaaa taaacaccca gggacgagca cctcatctgc
     6841 tggaataggg agaaaaactc aagggccgtt tgggtctcca cacttaccaa cagattcatg
     6901 tactgatctt cagcctgtac agaacagtac aatgtccaaa actcgttatc attactctat
     6961 gtgcccaaaa tgacccagat atacatcaac ccactacatc cttttgttct tgtttttttt
     7021 tttttttttt gctacttcga aaagaatttc cgtttagttg ctcttgctca tgtgctcaag
     7081 acggatgctg accgcccttc tgtgagcctc gagctcctca accttagcca actgcatgac
     7141 agcctggccg acgtttttga gaccctcggc agtcaagttg gagctggtaa tgtgcttgac
     7201 gaacgaggcg aaattgacgc cagagtactg cttgccaaag ccataggtag ctatgtgtct
     7261 tgacgttagc agaagaatct gtgaagcggg gctatttatg atatgtaact tacgcagcga
     7321 gtggttaaca ccagcagagt aatcgccaac ggactcagga gtccaggcgc caatgaagac
     7381 actaccggcg ttcatgacaa gatcgacagc cttctcggcc tccttgatct ggaggatcaa
     7441 gtgctcagga gcgtacttgt tgctgagctc catggcctcc tcgacggtct tgacctgcac
     7501 ggtgatcgag tgggcgatgg agccacggac aatctggacg cgaggaagct ccgtagcctg
     7561 acggtgaacc tcgtcctcaa tagcctgaag atgctcctcg tcgaggtcaa tagcgatcag
     7621 gatgacctga ctgtcaacgc cgtgctcagc ctgggacagg agatccgagg caacgaacgc
     7681 ggggttggcg tccttgtcag cgatgaccag cacctcggac gggccagcgg gcatgtcaat
     7741 accaacggca gcgttggtgt cgttgctgac gaacatcttg gcagcagtga cgaactggtt
     7801 accggggccg agaatcttgt cgaccttggt gatgctctcg gtgccgtagg ccatggcagc
     7861 tacggcctgg gcaccgccgg caagcacgat ggactcggcc ccaaccttgt gagcgacgtg
     7921 gacaatctcg ggagtgatgg ttccgtcggc gcggggagga gaggcgaaca caatcttgtt
     7981 gcagccggcg accatggcgg gaacacccag cataagggca gtgctgggga gaacggcggt
     8041 accgccgggg atgtagcagc cgacggcctc gatgggacga gagaaacggc tgcagacaac
     8101 accgggcatg gtctcgacct ggaggggctt ctcctccttc tgggcggcgt ggaacttgcg
     8161 gatgttctcg aaggacacgt cgatggcagc aatggtctcc tcagggagct gcataagctc
     8221 cttggggaag ggcgccttca ggacggggct agtaagagag gtagccttct cgaacttgtg
     8281 agtgtacgac agaacagcct tgtcgccgtt cttgcggacg tcctcgatga tggggacaat
     8341 gatcttgtag atggcatcgg acgacttttg cgcaggacgc ttgagagcag catcgagctc
     8401 ctcggtagag accttggagg cgtcgaaacg tctcatggtg atcttctcag gggtggtctc
     8461 ctgggcggcc tccttggtga caggggccga agtggcagcg agagcggacg ccgcaggctt
     8521 gatgccctcc ttctcagccc acttaccctt agcatctcca gtcctgcgct tgaccttcca
     8581 gctcttggcg tcaaggctcc tttcgatatc ggcaagagta acgccggcag caacggccct
     8641 ggtaagagca aagtagaaga gatcggcagc ctcaaaggcg atttcctggg gggtctgagc
     8701 ggtgcagagc tcctcagcct cctccatgat cttggcccgg actagcttct catcggagaa
     8761 gagacgggca gtgtaggagc cctcgggggc agactgtttc ctcgaaatca aagtctgctc
     8821 gagcttggga aggcctttga gctgaccaaa gcagccggac tgatcgaggt ggcagaaacg
     8881 acccttctgc ttcacgacaa acttgagagc atcgttatcg cagtcaagcg agatgcggac
     8941 gagctcctga gtgtctccgg aagtagcacc cttgtaccag agaccgcgct tccggctctg
     9001 atagacacca gtctgtgtcc tgagggcctc gttcacactc tcggcactgc tgtagaccag
     9061 acccagagca gtgtcgtgct catcaacgac aacggtgggg agaagaccat cgggacggtc
     9121 agacttccag acgctcgaga ggatggtgga gatggcaagc ttcagctgct cgagttc
//
