LOCUS       40924_30740        8294 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF332, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Expression and Visualization of Red Fluorescent Protein (RFP) in 
            Neurospora crassa
  JOURNAL   Fungal Genet. Newsl. 52 (2006) In press
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2005) Institute of Molecular Biology, University 
            of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA
REFERENCE   3  (bases 1 to 8294)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Newsl. 52 (2006) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2005) Institute of Molecular Biology, University of Oregon, 
            355 Streisinger Hall, Eugene, OR 97403-1229, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4056..4072
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4181..4858
                     /codon_start=1
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     promoter        complement(5614..5632)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(8293..8294,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac
     4021 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     4081 gatatcgaat tcgttaacag atctgcggcc gcggctcgag ggcgcgccaa ctagtgccgg
     4141 cggtaccacg cgtggccggc ctgctagcgg tagtggaggt atggtggcct cctccgagga
     4201 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
     4261 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     4321 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca
     4381 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc
     4441 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     4501 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agttccgcgg
     4561 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     4621 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     4681 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     4741 cgtgcagctg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     4801 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg
     4861 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacgtcat
     4921 caaagagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc acgagttcga
     4981 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     5041 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg
     5101 ctccaaggcg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc
     5161 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     5221 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagttcc gcggcaccaa
     5281 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
     5341 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
     5401 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
     5461 gctgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
     5521 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tgtagtctag
     5581 agcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat taagggcgtg
     5641 gacggctaat ggggtctgaa tgctaaaaga caccatttcc cacactccct ctgatttttt
     5701 ttatggtttt tttttatctt catttttcgc cgtcttttat tcggttgttt gggaggttca
     5761 catgcaacgt gcaagactgc aagttttttg agatgggcgc ttcatgcaat gcagcatcac
     5821 tgcagggaca ttgtgacatc cacgtttcga tcgtctgtgc ttcccccgtt catgtacgca
     5881 tgatctgaaa aaaaaaaaaa aaaaaaaggt tgatgtaata atgaaaataa acacccaggg
     5941 acgagcacct catctgctgg aatagggaga aaaactcaag ggccgtttgg gtctccacac
     6001 ttaccaacag attcatgtac tgatcttcag cctgtacaga acagtacaat gtccaaaact
     6061 cgttatcatt actctatgtg cccaaaatga cccagatata catcaaccca ctacatcctt
     6121 ttgttcttgt tttttttttt tttttttgct acttcgaaaa gaatttccgt ttagttgctc
     6181 ttgctcatgt gctcaagacg gatgctgacc gcccttctgt gagcctcgag ctcctcaacc
     6241 ttagccaact gcatgacagc ctggccgacg tttttgagac cctcggcagt caagttggag
     6301 ctggtaatgt gcttgacgaa cgaggcgaaa ttgacgccag agtactgctt gccaaagcca
     6361 taggtagcta tgtgtcttga cgttagcaga agaatctgtg aagcggggct atttatgata
     6421 tgtaacttac gcagcgagtg gttaacacca gcagagtaat cgccaacgga ctcaggagtc
     6481 caggcgccaa tgaagacact accggcgttc atgacaagat cgacagcctt ctcggcctcc
     6541 ttgatctgga ggatcaagtg ctcaggagcg tacttgttgc tgagctccat ggcctcctcg
     6601 acggtcttga cctgcacggt gatcgagtgg gcgatggagc cacggacaat ctggacgcga
     6661 ggaagctccg tagcctgacg gtgaacctcg tcctcaatag cctgaagatg ctcctcgtcg
     6721 aggtcaatag cgatcaggat gacctgactg tcaacgccgt gctcagcctg ggacaggaga
     6781 tccgaggcaa cgaacgcggg gttggcgtcc ttgtcagcga tgaccagcac ctcggacggg
     6841 ccagcgggca tgtcaatacc aacggcagcg ttggtgtcgt tgctgacgaa catcttggca
     6901 gcagtgacga actggttacc ggggccgaga atcttgtcga ccttggtgat gctctcggtg
     6961 ccgtaggcca tggcagctac ggcctgggca ccgccggcaa gcacgatgga ctcggcccca
     7021 accttgtgag cgacgtggac aatctcggga gtgatggttc cgtcggcgcg gggaggagag
     7081 gcgaacacaa tcttgttgca gccggcgacc atggcgggaa cacccagcat aagggcagtg
     7141 ctggggagaa cggcggtacc gccggggatg tagcagccga cggcctcgat gggacgagag
     7201 aaacggctgc agacaacacc gggcatggtc tcgacctgga ggggcttctc ctccttctgg
     7261 gcggcgtgga acttgcggat gttctcgaag gacacgtcga tggcagcaat ggtctcctca
     7321 gggagctgca taagctcctt ggggaagggc gccttcagga cggggctagt aagagaggta
     7381 gccttctcga acttgtgagt gtacgacaga acagccttgt cgccgttctt gcggacgtcc
     7441 tcgatgatgg ggacaatgat cttgtagatg gcatcggacg acttttgcgc aggacgcttg
     7501 agagcagcat cgagctcctc ggtagagacc ttggaggcgt cgaaacgtct catggtgatc
     7561 ttctcagggg tggtctcctg ggcggcctcc ttggtgacag gggccgaagt ggcagcgaga
     7621 gcggacgccg caggcttgat gccctccttc tcagcccact tacccttagc atctccagtc
     7681 ctgcgcttga ccttccagct cttggcgtca aggctccttt cgatatcggc aagagtaacg
     7741 ccggcagcaa cggccctggt aagagcaaag tagaagagat cggcagcctc aaaggcgatt
     7801 tcctgggggg tctgagcggt gcagagctcc tcagcctcct ccatgatctt ggcccggact
     7861 agcttctcat cggagaagag acgggcagtg taggagccct cgggggcaga ctgtttcctc
     7921 gaaatcaaag tctgctcgag cttgggaagg cctttgagct gaccaaagca gccggactga
     7981 tcgaggtggc agaaacgacc cttctgcttc acgacaaact tgagagcatc gttatcgcag
     8041 tcaagcgaga tgcggacgag ctcctgagtg tctccggaag tagcaccctt gtaccagaga
     8101 ccgcgcttcc ggctctgata gacaccagtc tgtgtcctga gggcctcgtt cacactctcg
     8161 gcactgctgt agaccagacc cagagcagtg tcgtgctcat caacgacaac ggtggggaga
     8221 agaccatcgg gacggtcaga cttccagacg ctcgagagga tggtggagat ggcaagcttc
     8281 agctgctcga gttc
//
