LOCUS       40924_30735        8291 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF331, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8291)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Expression and Visualization of Red Fluorescent Protein (RFP) in 
            Neurospora crassa
  JOURNAL   Fungal Genet. Newsl. 52 (2006) In press
REFERENCE   2  (bases 1 to 8291)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2005) Institute of Molecular Biology, University 
            of Oregon, 355 Streisinger Hall, Eugene, OR 97403-1229, USA
REFERENCE   3  (bases 1 to 8291)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Newsl. 52 (2006) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2005) Institute of Molecular Biology, University of Oregon, 
            355 Streisinger Hall, Eugene, OR 97403-1229, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4056..4072
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4099..4776
                     /codon_start=1
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     promoter        complement(5611..5629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(8290..8291,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac
     4021 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     4081 gatatcgaat tccaaaacat ggtggcctcc tccgaggacg tcatcaaaga gttcatgcgc
     4141 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     4201 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     4261 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggcgtacgtg
     4321 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg
     4381 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     4441 caggacggca cgctgatcta caaggtgaag ttccgcggca ccaacttccc ccccgacggc
     4501 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     4561 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     4621 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcagctgcc cggctactac
     4681 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4741 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc
     4801 agcggcagct ccggcaccgc ctcctccgag gacgtcatca aagagttcat gcgcttcaag
     4861 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4921 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     4981 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggcgta cgtgaagcac
     5041 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     5101 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     5161 ggcacgctga tctacaaggt gaagttccgc ggcaccaact tcccccccga cggccccgta
     5221 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     5281 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     5341 gagttcaaga ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg
     5401 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     5461 cgctccgagg gccgccacca cctgttcctg ggtagtggag gtggcgcgcc tactagtgcc
     5521 ggcacgcgtg gccggccagc tagtagatct gcggccgcgg ctcgagggta cctctagagc
     5581 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattaa gggcgtggac
     5641 ggctaatggg gtctgaatgc taaaagacac catttcccac actccctctg atttttttta
     5701 tggttttttt ttatcttcat ttttcgccgt cttttattcg gttgtttggg aggttcacat
     5761 gcaacgtgca agactgcaag ttttttgaga tgggcgcttc atgcaatgca gcatcactgc
     5821 agggacattg tgacatccac gtttcgatcg tctgtgcttc ccccgttcat gtacgcatga
     5881 tctgaaaaaa aaaaaaaaaa aaaaggttga tgtaataatg aaaataaaca cccagggacg
     5941 agcacctcat ctgctggaat agggagaaaa actcaagggc cgtttgggtc tccacactta
     6001 ccaacagatt catgtactga tcttcagcct gtacagaaca gtacaatgtc caaaactcgt
     6061 tatcattact ctatgtgccc aaaatgaccc agatatacat caacccacta catccttttg
     6121 ttcttgtttt tttttttttt ttttgctact tcgaaaagaa tttccgttta gttgctcttg
     6181 ctcatgtgct caagacggat gctgaccgcc cttctgtgag cctcgagctc ctcaacctta
     6241 gccaactgca tgacagcctg gccgacgttt ttgagaccct cggcagtcaa gttggagctg
     6301 gtaatgtgct tgacgaacga ggcgaaattg acgccagagt actgcttgcc aaagccatag
     6361 gtagctatgt gtcttgacgt tagcagaaga atctgtgaag cggggctatt tatgatatgt
     6421 aacttacgca gcgagtggtt aacaccagca gagtaatcgc caacggactc aggagtccag
     6481 gcgccaatga agacactacc ggcgttcatg acaagatcga cagccttctc ggcctccttg
     6541 atctggagga tcaagtgctc aggagcgtac ttgttgctga gctccatggc ctcctcgacg
     6601 gtcttgacct gcacggtgat cgagtgggcg atggagccac ggacaatctg gacgcgagga
     6661 agctccgtag cctgacggtg aacctcgtcc tcaatagcct gaagatgctc ctcgtcgagg
     6721 tcaatagcga tcaggatgac ctgactgtca acgccgtgct cagcctggga caggagatcc
     6781 gaggcaacga acgcggggtt ggcgtccttg tcagcgatga ccagcacctc ggacgggcca
     6841 gcgggcatgt caataccaac ggcagcgttg gtgtcgttgc tgacgaacat cttggcagca
     6901 gtgacgaact ggttaccggg gccgagaatc ttgtcgacct tggtgatgct ctcggtgccg
     6961 taggccatgg cagctacggc ctgggcaccg ccggcaagca cgatggactc ggccccaacc
     7021 ttgtgagcga cgtggacaat ctcgggagtg atggttccgt cggcgcgggg aggagaggcg
     7081 aacacaatct tgttgcagcc ggcgaccatg gcgggaacac ccagcataag ggcagtgctg
     7141 gggagaacgg cggtaccgcc ggggatgtag cagccgacgg cctcgatggg acgagagaaa
     7201 cggctgcaga caacaccggg catggtctcg acctggaggg gcttctcctc cttctgggcg
     7261 gcgtggaact tgcggatgtt ctcgaaggac acgtcgatgg cagcaatggt ctcctcaggg
     7321 agctgcataa gctccttggg gaagggcgcc ttcaggacgg ggctagtaag agaggtagcc
     7381 ttctcgaact tgtgagtgta cgacagaaca gccttgtcgc cgttcttgcg gacgtcctcg
     7441 atgatgggga caatgatctt gtagatggca tcggacgact tttgcgcagg acgcttgaga
     7501 gcagcatcga gctcctcggt agagaccttg gaggcgtcga aacgtctcat ggtgatcttc
     7561 tcaggggtgg tctcctgggc ggcctccttg gtgacagggg ccgaagtggc agcgagagcg
     7621 gacgccgcag gcttgatgcc ctccttctca gcccacttac ccttagcatc tccagtcctg
     7681 cgcttgacct tccagctctt ggcgtcaagg ctcctttcga tatcggcaag agtaacgccg
     7741 gcagcaacgg ccctggtaag agcaaagtag aagagatcgg cagcctcaaa ggcgatttcc
     7801 tggggggtct gagcggtgca gagctcctca gcctcctcca tgatcttggc ccggactagc
     7861 ttctcatcgg agaagagacg ggcagtgtag gagccctcgg gggcagactg tttcctcgaa
     7921 atcaaagtct gctcgagctt gggaaggcct ttgagctgac caaagcagcc ggactgatcg
     7981 aggtggcaga aacgaccctt ctgcttcacg acaaacttga gagcatcgtt atcgcagtca
     8041 agcgagatgc ggacgagctc ctgagtgtct ccggaagtag cacccttgta ccagagaccg
     8101 cgcttccggc tctgatagac accagtctgt gtcctgaggg cctcgttcac actctcggca
     8161 ctgctgtaga ccagacccag agcagtgtcg tgctcatcaa cgacaacggt ggggagaaga
     8221 ccatcgggac ggtcagactt ccagacgctc gagaggatgg tggagatggc aagcttcagc
     8281 tgctcgagtt c
//
