LOCUS       40924_30720        8606 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pMF276, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8606)
  AUTHORS   Honda S, Selker EU.
  TITLE     Tools for fungal proteomics: multifunctional neurospora vectors for 
            gene replacement, protein expression and protein purification
  JOURNAL   Genetics 182 (1), 11-23 (2009)
  PUBMED    19171944
REFERENCE   2  (bases 1 to 8606)
  AUTHORS   Honda S, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Institute of Molecular Biology, University 
            of Oregon, Eugene, OR 97403, USA
REFERENCE   3  (bases 1 to 8606)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8606)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2009"; volume: "182"; issue: "1"; pages: "11-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Institute of Molecular Biology, University of Oregon, 
            Eugene, OR 97403, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5016..5045
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      5568..5755
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5823..5839)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5869..5887)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(8605..8606,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatcccc gggttaatta acggtgaaca aaagctaatc tccgaggaag
     5041 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc gacggtgaac
     5101 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
     5161 tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa
     5221 agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga
     5281 acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt
     5341 tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg
     5401 gtgaacaaaa attaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga
     5461 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
     5521 aacaaaaatt aatcaatcac tagtgaattc gcgccacttc taaataagcg aatttcttat
     5581 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     5641 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     5701 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
     5761 ccgctaggga taacagggta atatagatcc cccgggctgc aggaattcga tatcaagctt
     5821 atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     5881 gttaattagg gcgtggacgg ctaatggggt ctgaatgcta aaagacacca tttcccacac
     5941 tccctctgat tttttttatg gttttttttt atcttcattt ttcgccgtct tttattcggt
     6001 tgtttgggag gttcacatgc aacgtgcaag actgcaagtt ttttgagatg ggcgcttcat
     6061 gcaatgcagc atcactgcag ggacattgtg acatccacgt ttcgatcgtc tgtgcttccc
     6121 ccgttcatgt acgcatgatc tgaaaaaaaa aaaaaaaaaa aaggttgatg taataatgaa
     6181 aataaacacc cagggacgag cacctcatct gctggaatag ggagaaaaac tcaagggccg
     6241 tttgggtctc cacacttacc aacagattca tgtactgatc ttcagcctgt acagaacagt
     6301 acaatgtcca aaactcgtta tcattactct atgtgcccaa aatgacccag atatacatca
     6361 acccactaca tccttttgtt cttgtttttt tttttttttt ttgctacttc gacatcaacc
     6421 cactacatcc ttttgttctt gttttttttt tttttttttg ctacttcgaa aagaatttcc
     6481 gtttagttgc tcttgctcat gtgctcaaga cggatgctga ccgcccttct gtgagcctcg
     6541 agctcctcaa ccttagccaa ctgcatgaca gcctggccga cgtttttgag accctcggca
     6601 gtcaagttgg agctggtaat gtgcttgacg aacgaggcga aattgacgcc agagtactgc
     6661 ttgccaaagc cataggtagc tatgtgtctt gacgttagca gaagaatctg tgaagcgggg
     6721 ctatttatga tatgtaactt acgcagcgag tggttaacac cagcagagta atcgccaacg
     6781 gactcaggag tccaggcgcc aatgaagaca ctaccggcgt tcatgacaag atcgacagcc
     6841 ttctcggcct ccttgatctg gaggatcaag tgctcaggag cgtacttgtt gctgagctcc
     6901 atggcctcct cgacggtctt gacctgcacg gtgatcgagt gggcgatgga gccacggaca
     6961 atctggacgc gaggaagctc cgtagcctga cggtgaacct cgtcctcaat agcctgaaga
     7021 tgctcctcgt cgaggtcaat agcgatcagg atgacctgac tgtcaacgcc gtgctcagcc
     7081 tgggacagga gatccgaggc aacgaacgcg gggttggcgt ccttgtcagc gatgaccagc
     7141 acctcggacg ggccagcggg catgtcaata ccaacggcag cgttggtgtc gttgctgacg
     7201 aacatcttgg cagcagtgac gaactggtta ccggggccga gaatcttgtc gaccttggtg
     7261 atgctctcgg tgccgtaggc catggcagct acggcctggg caccgccggc aagcacgatg
     7321 gactcggccc caaccttgtg agcgacgtgg acaatctcgg gagtgatggt tccgtcggcg
     7381 cggggaggag aggcgaacac aatcttgttg cagccggcga ccatggcggg aacacccagc
     7441 ataagggcag tgctggggag aacggcggta ccgccgggga tgtagcagcc gacggcctcg
     7501 atgggacgag agaaacggct gcagacaaca ccgggcatgg tctcgacctg gaggggcttc
     7561 tcctccttct gggcggcgtg gaacttgcgg atgttctcga aggacacgtc gatggcagca
     7621 atggtctcct cagggagctg cataagctcc ttggggaagg gcgccttcag gacggggcta
     7681 gtaagagagg tagccttctc gaacttgtga gtgtacgaca gaacagcctt gtcgccgttc
     7741 ttgcggacgt cctcgatgat ggggacaatg atcttgtaga tggcatcgga cgacttttgc
     7801 gcaggacgct tgagagcagc atcgagctcc tcggtagaga ccttggaggc gtcgaaacgt
     7861 ctcatggtga tcttctcagg ggtggtctcc tgggcggcct ccttggtgac aggggccgaa
     7921 gtggcagcga gagcggacgc cgcaggcttg atgccctcct tctcagccca cttaccctta
     7981 gcatctccag tcctgcgctt gaccttccag ctcttggcgt caaggctcct ttcgatatcg
     8041 gcaagagtaa cgccggcagc aacggccctg gtaagagcaa agtagaagag atcggcagcc
     8101 tcaaaggcga tttcctgggg ggtctgagcg gtgcagagct cctcagcctc ctccatgatc
     8161 ttggcccgga ctagcttctc atcggagaag agacgggcag tgtaggagcc ctcgggggca
     8221 gactgtttcc tcgaaatcaa agtctgctcg agcttgggaa ggcctttgag ctgaccaaag
     8281 cagccggact gatcgaggtg gcagaaacga cccttctgct tcacgacaaa cttgagagca
     8341 tcgttatcgc agtcaagcga gatgcggacg agctcctgag tgtctccgga agtagcaccc
     8401 ttgtaccaga gaccgcgctt ccggctctga tagacaccag tctgtgtcct gagggcctcg
     8461 ttcacactct cggcactgct gtagaccaga cccagagcag tgtcgtgctc atcaacgaca
     8521 acggtgggga gaagaccatc gggacggtca gacttccaga cgctcgagag gatggtggag
     8581 atggcaagct tcagctgctc gagttc
//
