LOCUS       40924_30710        8122 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pMF270, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Honda S, Selker EU.
  TITLE     Tools for fungal proteomics: multifunctional neurospora vectors for 
            gene replacement, protein expression and protein purification
  JOURNAL   Genetics 182 (1), 11-23 (2009)
  PUBMED    19171944
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Honda S, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Institute of Molecular Biology, University 
            of Oregon, Eugene, OR 97403, USA
REFERENCE   3  (bases 1 to 8122)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2009"; volume: "182"; issue: "1"; pages: "11-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Institute of Molecular Biology, University of Oregon, 
            Eugene, OR 97403, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5019..5108
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5141..5328
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5396..5412)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5442..5460)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(8121..8122,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatcccc gggttaatta acatctttta cccatacgat gttcctgact
     5041 atgcgggcta tccgtatgac gtcccggact atgcaggatc ctatccatat gacgttccag
     5101 attacgctgc tcagtgctga ggcgcgccac ttctaaataa gcgaatttct tatgatttat
     5161 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     5221 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     5281 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
     5341 ggataacagg gtaatataga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
     5401 ccgtcgacct cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
     5461 agggcgtgga cggctaatgg ggtctgaatg ctaaaagaca ccatttccca cactccctct
     5521 gatttttttt atggtttttt tttatcttca tttttcgccg tcttttattc ggttgtttgg
     5581 gaggttcaca tgcaacgtgc aagactgcaa gttttttgag atgggcgctt catgcaatgc
     5641 agcatcactg cagggacatt gtgacatcca cgtttcgatc gtctgtgctt cccccgttca
     5701 tgtacgcatg atctgaaaaa aaaaaaaaaa aaaaaggttg atgtaataat gaaaataaac
     5761 acccagggac gagcacctca tctgctggaa tagggagaaa aactcaaggg ccgtttgggt
     5821 ctccacactt accaacagat tcatgtactg atcttcagcc tgtacagaac agtacaatgt
     5881 ccaaaactcg ttatcattac tctatgtgcc caaaatgacc cagatataca tcaacccact
     5941 acatcctttt gttcttgttt tttttttttt tttttgctac ttcgaaaaga atttccgttt
     6001 agttgctctt gctcatgtgc tcaagacgga tgctgaccgc ccttctgtga gcctcgagct
     6061 cctcaacctt agccaactgc atgacagcct ggccgacgtt tttgagaccc tcggcagtca
     6121 agttggagct ggtaatgtgc ttgacgaacg aggcgaaatt gacgccagag tactgcttgc
     6181 caaagccata ggtagctatg tgtcttgacg ttagcagaag aatctgtgaa gcggggctat
     6241 ttatgatatg taacttacgc agcgagtggt taacaccagc agagtaatcg ccaacggact
     6301 caggagtcca ggcgccaatg aagacactac cggcgttcat gacaagatcg acagccttct
     6361 cggcctcctt gatctggagg atcaagtgct caggagcgta cttgttgctg agctccatgg
     6421 cctcctcgac ggtcttgacc tgcacggtga tcgagtgggc gatggagcca cggacaatct
     6481 ggacgcgagg aagctccgta gcctgacggt gaacctcgtc ctcaatagcc tgaagatgct
     6541 cctcgtcgag gtcaatagcg atcaggatga cctgactgtc aacgccgtgc tcagcctggg
     6601 acaggagatc cgaggcaacg aacgcggggt tggcgtcctt gtcagcgatg accagcacct
     6661 cggacgggcc agcgggcatg tcaataccaa cggcagcgtt ggtgtcgttg ctgacgaaca
     6721 tcttggcagc agtgacgaac tggttaccgg ggccgagaat cttgtcgacc ttggtgatgc
     6781 tctcggtgcc gtaggccatg gcagctacgg cctgggcacc gccggcaagc acgatggact
     6841 cggccccaac cttgtgagcg acgtggacaa tctcgggagt gatggttccg tcggcgcggg
     6901 gaggagaggc gaacacaatc ttgttgcagc cggcgaccat ggcgggaaca cccagcataa
     6961 gggcagtgct ggggagaacg gcggtaccgc cggggatgta gcagccgacg gcctcgatgg
     7021 gacgagagaa acggctgcag acaacaccgg gcatggtctc gacctggagg ggcttctcct
     7081 ccttctgggc ggcgtggaac ttgcggatgt tctcgaagga cacgtcgatg gcagcaatgg
     7141 tctcctcagg gagctgcata agctccttgg ggaagggcgc cttcaggacg gggctagtaa
     7201 gagaggtagc cttctcgaac ttgtgagtgt acgacagaac agccttgtcg ccgttcttgc
     7261 ggacgtcctc gatgatgggg acaatgatct tgtagatggc atcggacgac ttttgcgcag
     7321 gacgcttgag agcagcatcg agctcctcgg tagagacctt ggaggcgtcg aaacgtctca
     7381 tggtgatctt ctcaggggtg gtctcctggg cggcctcctt ggtgacaggg gccgaagtgg
     7441 cagcgagagc ggacgccgca ggcttgatgc cctccttctc agcccactta cccttagcat
     7501 ctccagtcct gcgcttgacc ttccagctct tggcgtcaag gctcctttcg atatcggcaa
     7561 gagtaacgcc ggcagcaacg gccctggtaa gagcaaagta gaagagatcg gcagcctcaa
     7621 aggcgatttc ctggggggtc tgagcggtgc agagctcctc agcctcctcc atgatcttgg
     7681 cccggactag cttctcatcg gagaagagac gggcagtgta ggagccctcg ggggcagact
     7741 gtttcctcga aatcaaagtc tgctcgagct tgggaaggcc tttgagctga ccaaagcagc
     7801 cggactgatc gaggtggcag aaacgaccct tctgcttcac gacaaacttg agagcatcgt
     7861 tatcgcagtc aagcgagatg cggacgagct cctgagtgtc tccggaagta gcacccttgt
     7921 accagagacc gcgcttccgg ctctgataga caccagtctg tgtcctgagg gcctcgttca
     7981 cactctcggc actgctgtag accagaccca gagcagtgtc gtgctcatca acgacaacgg
     8041 tggggagaag accatcggga cggtcagact tccagacgct cgagaggatg gtggagatgg
     8101 caagcttcag ctgctcgagt tc
//
